...benefit from some Addgene support? Please get in touch, as we aim to support a couple of events a year...across Europe get introduced to the cultures and opportunities available to them at university. At a Freshers... into university life. The fairs are also an opportunity for the societies to promote their upcoming events...biological societies that Addgene has had the opportunity to work with at Freshers Fairs in the past year...undergraduates and PhD students so that they can help support each other and provide advice. The society organizes...involved with. The society provides talks and career support for a range of different scientific disciplines... in its first year. The society facilitates opportunities to learn about Synthetic Biology and exciting...

...et al. Cell. 2015. PubMed PMID: 26627737 pORTMAGE: A portable method for bacterial genome engineering ...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...their enhanced TANGO arrestin recruitment assay incorporating 314 codon-optimized GPCR sequences for human...MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand DNA (ssDNA) oligonucleotides...so-called “genomically recoded organism” (3) but its portability to other bacterial strains remains seriously ...bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses the λ Red recombinase enzymes, as well...can be used in a broad range of strains. Thus, pORTMAGE simultaneously allows genome editing and mutant...

...plasmids, are export-controlled materials in the USA, where Addgene is located.  The export license, if ...requestors. We also offer export license applications for VSV and SARS-COV-1 plasmids. Export control license ...Addgene is excited to announce we now offer an export license application for rabies materials! Some ...license process    An export control license, once approved, allows Addgene to ship plasmids or viral vectors...the applicable research project when purchasing export-controlled materials. Addgene will then apply for...process.   Due to recent regulation changes, an export control license for rabies materials may be required...required for others. You will be notified if an export license is not required for your country after ...

...You need the support of your institute to do this one. Universities will usually only support tenure track...Further Evidence (RFE). Letters of support are the only opportunity that you are given to present your...and so on. Candidates should look broadly at opportunities to develop skills related to their areas of ...must have both substantial merit and national importance; (2) The foreign national must be well positioned...evaluating an NIW, USCIS will also focus on the importance of the individual’s work in their field and the...working under in the lab or has won, awards, media reports, and membership in groups related to the foreign...This criterion is best proven through letters of support from experts in the field. You must also be able...

...Fluorecent Proteins in Nano Lanterns Reporter gene assays A reporter gene, such as luciferase, usually serves... is also the most commonly used bioluminescent reporter. This beetle emits a yellow-green light with a...several studies utilized luciferase as a genetic reporter in plant and mammalian cells. Luciferase assays...available to choose from) is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In...transcription within cells, where detection of the reporter protein or its enzymatic activity is measured....expression can be determined by detection of the reporter in a specific assay, which ideally would have .... Production of photons by this bioluminescent reporter occurs slower than fluorescent-based methods, ...

...STEAMid. Internships open doors to a web of opportunities that the classroom cannot offer. Furthermore... not deter any student from applying to the opportunities of their dream.  To help students focus on the...below:  1. Eligibility check Scrutinize every opportunity prior to starting an application to assess your...unless you are applying for a Federal Government opportunity, which tends to be more detailed. Beyond the ...draft a personal statement, which is the perfect opportunity to paint a comprehensive picture of your academic...statement will pay dividends. This is the only opportunity where you get to impress the judges on your ability...your recommenders deliver a superb and detailed portrayal of yourself. Be mindful that January and February...

...molecular brightness (Table 1).  NIR reporters and biosensors NIR reporters can tell us about cellular events..., D. & Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions...FPs) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically. Fortunately...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in... a cell and depends on the efficiency of BV incorporation by an apoprotein and protein expression level...higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1). In general...Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their small size (17...

...it is incorporated. We were surprised to see how rapidly the efficiency of mutation incorporation falls...contains the intended sequence changes to be incorporated into the edited genome. In mammalian cells such...these two strategies allows you to specifically incorporate homo- or heterozygous mutations. 1. Increasing...therefore strongly encourage any researcher to incorporate CRISPR/Cas-blocking mutations at both targeted... our frustration) in many cases we only saw incorporation of the blocking mutation. Despite the fact that... away from the cut site were not efficiently incorporated during HDR. We decided to systematically characterize...only about 30 bp, it is no longer feasible to incorporate mutations without screening thousands of clones...

...expression, sometimes incorporating chemically inducible approaches to provide temporal control, this new ...random variation, or noise, in reporter expression. To do so, one places reporter expression under the control...expression of a reporter protein, it is possible to change the amount of noise in reporter gene expression...can deliver these components together using electroporation. In these bulk cell experiments, Clift et al...no stress or damage upon TRIM-Away, and the electroporated cells re-adhered to the culture plate within...deliver the target antibody. TRIM-Away reagents and reporters are available from Addgene - we hope they will...lab has deposited plasmids encoding fluorescent reporters and other proteins controlled by GAL4 and ZIF268...

...emphasizes that reporting the sex of cells and tissues—when that information is reliable—is important to ensuring...Universities need to incorporate these methods into their curricula. Corporations need to integrate these...Governments, universities, and, increasingly, corporations in the US and Western Europe have taken three...into research. Why are sex and gender analysis important? Doing research wrong costs lives and money. According...positive, the analysis did find 263,000 more osteoporotic fractures. The overall economic savings to the... goal of gendered innovations It is crucially important to get the research right from the beginning. ...unique to male stem cells. Nor did they detect important differences in function between male and female...

...gene. Spatio-temporal expression of optogenetic reporters: Since most optogenetic reporters are delivered...invert the reporter and GeneR fragment. Then the second recombination event would excise the reporter from ...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ...Cre-dependent reporter expression, the potential for background expression of the reporter will be greatly...shown in Figure 2, the cassette should contain a reporter DNA sequence (such as GFP) in the sense orientation...the two pairs of target sites. By flanking the reporter with one loxP site and one lox511 site in the ...would ensure that the reporter remains silent until a cell or tissue-specific Cre is provided. Since the FLEx...

...sequence are important considerations for designing your gRNAs. For indels, it's not so important what location...nom. Me love gRNAs! Comic by Maya Kostman.   Important considerations before you start an experiment ...tool among them. While this seems obvious, it is important to remember that the same is true when designing...location in the gene you target, but it is important that your gRNA sequence is designed to be highly active...CRISPRi, these considerations are of roughly equal importance (target should be near the TSS but you can worry...from). Finally, for HDR, location is much more important because you have to target within ~30 nt of your...gRNA with an optimized sequence is of primary importance (more on this below). Created with BioRender.com...

...along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to ...sometimes advantageous, particularly when only a portion of the plasmid is packaged for viral delivery, ...expression levels between two or more genes is important. How do multicistronic vectors work? Scientists...single transcript. The two strategies most widely incorporated into plasmids for research purposes are described... using IRES elements. Further, scientists have reported lower expression of the downstream cistron due...eukaryotic cells, and, although some scientists report close to 100% cleavage with some of these peptides...that already has the multicistronic element and reporter cloned in. In these plasmids you would simply ...

... people usually report? Addgene and other viral vector manufacturing facilities report the physical titer... characteristics give us information on three important factors. What can AAV titers tell you? Physical...cell transduction assays. Wild-type AAV2 has been reported to have a near-perfect physical-to-infectious ... However, for recombinant AAV2, the same study reported a physical-to-infectious particle ratio of 50:...(“full”) (Zeltner et al., 2010), while another reported that only 20% of recombinant AAV2 particles were...vector preparation protocol, and we are pleased to report that over 95% of viral capsids in representative...dosing purposes in preclinical studies, it is important to understand what these values mean and how they...

...of the independent variable you are testing. Importantly, they demonstrate the functionality of your experimental... experimental system and help identify opportunities for troubleshooting or optimization within your experiment...non-independent variables.  To illustrate the importance of control plasmids, we will take a step beyond...may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either... well of your cells. Regardless, the amount of reporter protein activity correlates to both the amount...living, viable cells. This result presents an opportunity for troubleshooting and optimization of the transfection... transfection reagents and process. It is important to note that optimal experimental conditions, including...

...milestone. Viral service supporters We would like to thank the companies who supported this project with funding...and be ready to provide extensive logistics support for export and shipping on dry ice. The Business and...seed money was used to build a completely self-supporting service so that Addgene is not dependent on grants... in accelerating the Viral Service. With their support we are able to provide faster access to a larger...number of viral preps. Visit our Sponsors and Supporters Page Thank you to all of our generous, sharing...Addgene repository. Without these scientists and the support of their technology transfer offices, Addgene would...Jonathan Ting’s lab from the Allen Institute have supported the AAV initiative from the beginning and have...

...Antibody Data Hub. Data reports  Each antibody submission is used to create a data report, which contains experimental...that information in the report as well.    Figure 3: An antibody data report, showing the results of ...methods section for the data report shown in Figure 3.   At the end of the report, you’ll find a results section... the data report shown in Figure 3. The antibody is rated "Pass". Curating the data reports Just like... page of the Addgene Data Hub.   Antibody data reports can be searched and sorted by gene/target, name...information is then formatted and published as a report with a DOI for citation. If you’re considering ...

...Career Development; a Stakeholder Workshop Report.” The report enabled the development of priorities for...programs are still doing an inadequate job of supporting science trainee transition into diverse, successful...amplified by systemic biases which, in the absence of support and structure, prevent science from attracting ...Development Hub Workshop Attendees and their 2021 report. Image from pd|hub.   To begin to tackle these... of Massachusetts Medical School. It has been supported by a Steering Committee and working groups with...the findings were captured in this just released report “Enhancing Dissemination of Evidence-Based Models...Institute of General Medical Sciences (NIGMS) will support the development and implementation of collections...

... and logistic support to increase the chances of success, but also nurture a supportive and protective...Instead, talent should be realized through equal opportunities for all regardless of origin. It is therefore...first-hand vision of the challenges and career opportunities that truly exist outside their immediate frontiers...mentee, where (s)he feels truly understood and supported, and can more fully identify with and be inspired...the beginning, I registered at IMP to have an opportunity to improve my networking with mentors who are...how to write cover letters, and has suggested important things to emphasize in order to be selected in...“IMP showed me a wide range of professional opportunities that are outside of our typical boundaries, ...

...can be gained by understanding the location and transport of the protein within a living cell. Analyzing...Analyzing differences in protein localization and transport between healthy and diseased states can also provide...endoplasmid reticulum, modified in the golgi and then transported in vesicles via the secretory pathway to their...the end of their life cycle, proteins may be transported through the vesicles to the lysosome where they...Once you've created your fusion protein, it's important to validate its function relative to the untagged... of an organelle or involved in intracellular transport pathways, it is possible to map the internal organization...used markers for major mammalian organelles and transport pathways. It is best, though not always possible...