We narrowed to 630 results for: proc

...often, it is also a bottleneck in the experimental process. The majority of current cloning techniques involve...bacterial recombination pathway to allow any cloning procedure to be performed using a simple two-step, 2 hr ...be immediately adopted by any laboratory. All procedures are performed using the same three step protocol... greater homology, so performing more complex procedures can be aided by extending homologous sequences...routinely screen just two colonies for simple cloning procedures. Use high fidelity DNA polymerase This will minimize...

...plants doesn’t always have to be a time-consuming process. While developing transgenic hairy root lines in... longer, a transient plant leaf transformation process could save the plant biologist some time… months...This method is commonly used in C. roseus, but processes specific to photosynthetic active tissues cannot... In the laboratory we can utilize this natural process by replacing the genes on the T-DNA region with...other aspects of the transient transformation procedure (Mortensen et al., 2019). Our method might provide...

...Addgene’s MoClo guide for all the basics of the process. Also refer to Figure 1 for a visual overview! ...Figure 1: General overview of the MoClo assembly process to produce a multigene construct. Figure exemplifies...choices you make during the early stages of the process. Some questions to start with are: Is your sequence...different levels of your MoClo assembly. Know your process The MoClo system allows for directional assembly...request the sites to be domesticated through the process, and request your sequence with the appropriate...

...to cancer, premature aging, and other harmful processes. The most dangerous type of DNA lesion results...the more accurate mechanism for DSB repair. The process is generally error-free provided the DNA template...intermediates can then resolve to complete the DNA repair process Homology directed repair pathways HDR mechanisms...displaced from the template and returned to the processed end of the non-invading strand at the other DSB...resulting ssDNA. A single HJ forms during this process and resolves by cleavage of the crossed strand....

...primary antibodies is a time consuming and expensive process. Consider the vast number of possible primary antibodies...Performing an immunofluorescence experiment The process to perform IF can be broadly divided into the several...High-level overview of an indirect immunofluorescence procedure with key considerations about each step. Image...specific 3-dimensional epitopes. Different sample processing steps can alter epitopes in different ways, so...secondary antibody you use, so as long as the sample processing steps used in a non-fluorescent IHC/ICC assay...

...without the need for time-consuming purification processes, and are powerful, due to their ability to integrate...three separate enzymes that are proteolytically processed from the larger protein: PR (protease), RT (reverse...delivered to cells, the viral life cycle initially proceeds as usual. Reverse transcription of the transgene...Figure 2: Schematic representation of integration process and episomal forms of non-integrating transgenes...affecting the other steps of transduction.  The process for producing your own IDLVs is nearly identical...

... mutagenesis vastly facilitates the subsequent process of screening for succesfully mutated clones. Forward... introduce undesired mutations through the PCR process, you need a high-fidelity polymerase. There are...fragment length polymorphism (RFLP) analysis. In this process, if your mutation introduces an additional restriction...DNA duplex stability from the base sequence." Proceedings of the National Academy of Sciences 83.11 (1986...Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase for site-directed...

...doing an experiment wrong. However, even simple procedures like pouring a plate or running a gel have intricacies... retain searchable, thorough notes on how lab procedures were actually performed, potentially curtailing... sense on many different levels - it’s hard to process large amounts of information and, from a SciComm...how and why papers change throughout the review process. This will hopefully push reviewers towards more...experimental design as part of the publication process (Nosek et al 2018). In this ideal situation, a ...

...generation easi-er! Generating mice before CRISPR The process of generating knock-in mice pre-CRISPR was very...would be unsuccessful. CRISPR made the overall process of mouse model generation simpler, as described...knockout alleles or point mutations, the 8-10 month process of conventional gene targeting is lowered to about...embryo to create chimeric mice. This laborious process takes ~8-10 months. With CRISPR (B), you can inject...than i-GONAD and is a more technically difficult process. Overall, the main drawback of i-GONAD is that ...

...specific subset of cells in a population in a procedure termed fluorescence activated cell sorting or ...forces the cells to line up in single file in a process termed hydrodynamic focusing. This allows each ...factors including sample preparation, staining procedures, and controls. To prevent instrument clogs, ensure...control with cells that went through the staining procedure minus the antibody. An unstained control allows... to aid with compensation. Compensation is the process of correcting spectral overlap that occurs between...

...this blog post, we'll discuss the principles and process of designing a flow panel.  Principles of excitation...electrons within atoms to a higher energy state. This process is called excitation. After a short period, the...spectrum compared to APC. This happens due to a process called Fluorescence Resonance Energy Transfer (...constantly happening in cell culture, the transduction process itself also leads to some cell death. As a result...be absorbed and emitted by matter, and how this process creates visible colors. Next, you learned about...

...the Red2Green processing facility, where it’s sanitized through an EPA-approved process and no longer ...focused on streaming some lab waste for recycling- a process that’s integrated into waste management in our ...we consider the environmental impact of those processes? Who even regulates them? You may be surprised...

...capability with the endogenous tRNA-processing system.” Proceedings of the National Academy of Sciences...short answer is no. Unless you use a system for processing a continuous multi-gRNA transcript, each gRNA... Note: depending on which method you use, the procedure will vary slightly.   Two Golden Gate options...and somatic genome engineering in Drosophila.” Proceedings of the National Academy of Sciences USA 111(29...

... planning, visualizing, and documenting their procedures. Preventable errors in the design of cloning ...of software with these qualities is an ongoing process that involves iterative refinements in response... algorithm and thereby speed up the annotation process. Many of the best ideas come from engaging with...use SnapGene during the plasmid quality control process Check out full plasmid sequencing at Addgene Additional...

... Hot Plasmids Podcast!  MarathonRT: A highly processive reverse transcriptase Article contributed by ...several important shortcomings including low processivity (ie. the probability of the polymerase continuing...heterogeneous RNA samples. Furthermore, such high processivity enzymes and the structural understanding thereof...to existing technologies based on polymerase processivity. Please find the MarathonRT plasmid here! Zhao...

...Direct reprogramming describes the process where differentiated cells are turned into a cell type of ...medicine, direct reprogramming is an inefficient process, with the majority of cells failing to develop ...expression of transcription factors Foxa1 and Hnf4α, a process that like other reprogramming protocols is inefficient...CellTag could be used to study other biological processes where cells are undergoing identity changes, such...

...sample and target type. To start the validation process, one must first confirm that a candidate antibody...approved antibody clones The antibody validation process represents a high level of effort and time, which.... We find it useful to start with a screening procedure in easily genetically modified cell types (top... C. (2023). Scaling of an antibody validation procedure enables quantification of antibody performance...Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene...

...systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA guides required...scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived from...repeat regions contain sequences required for processing pre-crRNA into mature crRNA and tracrRNA binding... Overview of native CRISPR arrays and their processing for cleaving foreign DNA. A) CRISPR arrays as...

...came to MIT to interview, part of the interview process was several short talks by current faculty members... it was just this complete black box. The RNAi process had just been discovered, the mechanism was barely...what confers specificity in the miRNA regulatory process. This early research is still relevant to his work...determining what genes are involved in biological process X, you can do with CRISPR technology...” and “with...

...is unlikely to be caught during the peer review process, which often focuses more attention on the results...noise There is, of course, simple noise in the process of sampling and inference-making. Even perfect ...exist or are preserved as part of the publishing process and become lost if not archived. Remove bias with...with preregistration Preregistration is the process of creating a time-stamped research plan in a public...