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We narrowed to 396 results for: promoter

Showing: 141 - 160 of 396 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...to measure time. These plasmids with different promoters driving expression of Cas9 were used in this study...ready-to-use AAV vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with...

  3. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...Addgene are perfect for expressing GFP from a given promoter, and the technique can be adapted to other transgenes...observed will be dependent on both the endogenous promoter and the 3’ UTR of the locus of interest, since...

  5. Call for Guest Bloggers

    Type
    Blog Post
    ...and Resumes. An Expanded Network - Addgene will promote your blog on a variety of social media outlets ...

  6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    .... MazF expression is under control of the AOX1 promoter whose expression is induced by methanol. Once ...luciferase-expressing plasmids to examine in vivo-induced promoters in the Vibrio cholerae vaccine strain CVD 103-...

  8. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...release of the stress hormone norepinephrine to promote vigilance. A recent study investigated the injection...Tracy L. Bale. "Dorsal raphe neuroinflammation promotes dramatic behavioral stress dysregulation." Journal...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    ...hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids include a mRFP reporter... nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots. Find the...

  11. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...a chimeric 5’ LTR containing a Tat-independent promoter. Although safer, this system may be more cumbersome... vectors integrate into the genome, they could promote oncogenesis by altering local gene expression. ...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...contains a reporter gene cloned downstream of a promoter containing the target sequence of the DBD. The...as the “prey”) in close enough proximity to the promoter of the reporter gene to activate expression (Figure...

  14. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...types, as CAG is a ubiquitous and constitutive promoter. Use this for doxycycline inducible (tet-off) ...

  17. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...10878/ . Description Vector Element U6 Human U6 promoter drives RNA Polymerase III transcription for generation...transduced cells. hPGK Human phosphoglycerate kinase promoter drives expression of puromycin. Puro R Puromycin...

  18. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...(which you can filter by serotype, species, or promoter) can save you significant time and money. For ...

  19. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... pages, we added curation fields for purpose, promoters, expression types, and more, all of which help...How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to Verify Your Plasmid ...

  20. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... vector is inserted downstream of a functional promoter (gene traps). The resultant gene targeting system...pAAV-TK-Acceptor has a conventional heterologous promoter-driven TK-neoR gene. To modify these tagging vectors...pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances...
Showing: 141 - 160 of 396 results