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We narrowed to 398 results for: promoter

Showing: 141 - 160 of 398 results
  1. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...resources and information about organizations promoting reproducibility in this booklet (which was also...line we may see further adoption of policies that promote reproducible publishing practices. One example ... also hopefully see a shift toward hiring and promotion practices that focus on reproducibility and ensuring... Including descriptions of many organizations promoting reproducibility List of Tools for Reproducibility...
  2. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of...channel, such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining...anion channel, such as the glycine receptor, will promote hyperpolarization. PSAM ligands The advantage of...
  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...to measure time. These plasmids with different promoters driving expression of Cas9 were used in this study...ready-to-use AAV vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with...
  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ... cells where common eukaryotic promoters (such as CMV or EF1A promoters found in many CRISPR plasmids)...transfection or viral transduction     Appropriate promoters must be chosen for both Cas9 and gRNA expression...
  5. SunTag and Fluorescent Imaging

    Type
    Blog Post
    .... More transcription factor binding sites in a promoter lead to higher transcriptional activation. Multiple...
  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ...hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids include a mRFP reporter... nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots. Find the...
  7. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    .... MazF expression is under control of the AOX1 promoter whose expression is induced by methanol. Once ...luciferase-expressing plasmids to examine in vivo-induced promoters in the Vibrio cholerae vaccine strain CVD 103-...
  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...a chimeric 5’ LTR containing a Tat-independent promoter. Although safer, this system may be more cumbersome... vectors integrate into the genome, they could promote oncogenesis by altering local gene expression. ...
  9. RUBY-Red Siliques

    Type
    Blog Post
    ...of these three enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator...
  10. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...extreme self-starters and especially good at self-promoting—No one is going to tell you to pick up the pace...diversity and acceptance can be an issue in getting promoted (especially race and gender) Interpersonal issues...
  11. Lentivirus Production

    Type
    Protocol
    .... Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...
Showing: 141 - 160 of 398 results