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We narrowed to 916 results for: tro

Showing: 141 - 160 of 916 results

  1. Fluorescent Protein Guide

    Type
    Collection
    ...Plasmids | Guide Use light to detect, measure, and control molecular signals, cells, or groups of cells with...

  4. All Antibodies

    Type
    Collection
    ...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

  5. Plasmid Collections

    Type
    Collection
    ...Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated Topics ...

  6. Chemogenetics Plasmids

    Type
    Collection
    ...specific small molecules, allowing a scientist to control signaling within cells expressing these engineered...

  8. Antibody Plasmid Collection

    Type
    Collection
    ...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...enzymes and related tools are available: PE2 - introduction of five mutations in RT enzyme PE3 - PE2 plus...

  10. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...vectors to express your gene of interest under the control of a variety of promoters. Professor Oka and the...

  11. TALEN Guide

    Type
    Collection
    ...new technology, and we hope to continue to have a strong scientific partnership with both the labs that ...

  12. Arf GTPase Family

    Type
    Collection
    ...traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid droplets, the nucleus...

  13. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Yannick Doyon's lab deposited a plasmid which introduces an N- or C- terminal affinity tag (3xFLAG-2xSTREP...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...specificity, or retrograde transport in neurons. (b) Genetic cargo can be customized to control transgene expression... Capsid Published Tropism Model Organism Mouse Strain-Specific Tropism?  Reference AAV-...and astrocytes Mice No (LY6C1)   Hanlon et al., 2019 AAV-BI-28 CNS: neurons, astrocytes, oligodendrocytes...-Specific Tropism?" column. For a complete list of mice strains with LY6A-dependent tropism, see S3 table...derived from AAV9 using iTransduce, shows tropism toward astrocytes and neurons, with greatly increased efficacy... appears to show a broader tropism with a potential bias toward astrocytes (Chuapoco et al., 2023). Additionally...systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates. Nature Communications...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...developed a new AAV variant, rAAV2-retro, that migrates retrogradely at higher rates than any other AAV... AAV2-retro vectors with a variety of transgene/promoter combinations. Check out our retrograde AAV blog...requires both antero- and retrograde labelling, synaptic restriction as well as controlled monosynaptic and polysynaptic...Selective retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its retrograde axonal...bodies to the terminals of their axons; while retrograde tracing outlines neurons in the opposite direction...axons to their cell bodies. Anterograde and retrograde tracing take advantage of existing transport ...myosin, and enzymes for transmitter synthesis. Retrograde transport is used for transport of endocytosed...

  16. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... What kind of control? On, off, tunable, and/or inducible? How long do you need control? Will temporary...temporary control (a few days) be enough, or do you want a permanent control mechanism? Where is the control...transcription instead of introducing a DNA double strand break. Transient introduction of CRISPRi or inducible...vector! On/off control all the time If your experimental system requires tunable control - or maybe you...price of tunable control These systems can be easily implemented for exogenously introduced proteins, as ...prior to introduction of the construct into cells. However, if you would like tunable control of an endogenously...forever? You don’t need to resort to the force to control protein expression in mammalian cells: easy to ...

  17. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus Retroviruses...type of gamma-retrovirus is Murine Leukemia virus (MLV). Naturally isolated gamma-retroviruses have been ...Lentivirus is a type of retrovirus and shares many common features with gamma-retrovirus including genome architecture... nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus are so closely related...expression (Robinson, 1982). To add insult to injury, retroviruses generally have moderate to high immunogenicity...immune response in the host. So why use a gamma-retrovirus in the lab? Well, they are essentially evolutionarily... types with a high efficiency. However, gamma-retrovirus infection is restricted to dividing cells only...

  18. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...A common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain...PSD95-mcherry) under the control of Cre recombinase. After in-utero electroporation, this plasmid mix results...tracking for instance) can be controlled with FLP-FRT. For further temporal control, Tamoxifen inducible Cre...system, but it only gives you a single level of control without modification - either Cre is there or it...for site specific (and often cell type specific) control of DNA recombination and gene expression can be...addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed. Read...FLP and Cre enable additional levels of genetic control. What is FLP FRT and how does it work? FLP recombinase...

  19. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...Include proper controls To ensure accurate data analysis, include a complete set of controls. Ideally, every...will have a negative control that does not express the target and a positive control that does. The negative...of experiments easier and faster to perform. Introduction to flow cytometry Flow cytometry allows users... (3): An optics system A fluidics system An electronics system  Cells are first resuspended in a pressurized...fluorophore or fluorochrome-stained protein will emit a stronger photon than a cell expressing low levels of the... further the shift of a cell from the negative control, the higher the level of expression. A routine ...triton-x, saponin, or tween. Take care when using strong detergents like triton-x and tween as extended ...

  20. Important Considerations When Using AAVs

    Type
    Blog Post
    ...larger amount of vector using a serotype with strong CNS tropism (4). If axonal transport is not desirable...receptor physiology and the effects of metabotropic and ionotropic influences on overall cell function. ... thanks to our guest blogger Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student at ...post was contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University...known is that different serotypes have different tropisms but, as you can see, AAVs of each serotype can... asking questions!  Table 1: AAV Serotypes and Tropism Experiment  Detection Method CNS AAV1, AAV2...>AAV8>AAV6>AAV5>AAV1>AAV2 This preferential tropism for different brain regions is largely due the ...
Showing: 141 - 160 of 916 results