We narrowed to 537 results for: val

...using HR was found to block the acquisition and retrieval of contextual fear memories [5].  In a modified...other regions of the brain may be involved in retrieval of remote contextual memories. This study is an...real-time inhibition), and behavioral (remote memory retrieval) specificity. Optogenetics can be used to more...2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote memories. Cell. 2011;147...

...condensed into a statistically evaluated set of values.  However, to evaluate the interpretation of the experiment...a peer-reviewed repository for “scientifically valuable” datasets. It likely won’t be long before other...

...Biotin is a cofactor that in its active form is covalently attached to the active site of key metabolic ...in cells and is the process by which biotin is covalently bonded to a specific lysine at the active site...the bond between them is one of the strongest noncovalent protein-ligand interactions found in nature. ...in live mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534...

... in fusion proteins. Since GFP is such a well-validated, all-round good performing probe, it is the first...different acid concentrations. Overviews of the pKa values of different fluorescent protein are available ...remain fluorescent under acidic conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1...Another peptide-based strategy uses an enzyme to covalently label the peptide with a fluorophore. Both systems...

...help of the DNA tuner program) (Latynski and Valentovich 2014). We routinely clone the purified amplicons...gentamicin-resistance (amplified from TP997). We validated these vectors, which we call “pTOX” in a broad...turn subtly blue or magenta. This is particularly valuable when first optimizing conjugation in strains that...PMID: 25800749. PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer program for the construction...

...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the challenges... in DMD patient myoblasts. They observed a 13% removal rate of exon 51, which resulted in appropriately...Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research ...

...work begin — validation, validation, validation! (psst… don't forget to submit your validation data to our...the mechanism of entry if known, and, as always, validate the capsid for your specific application and animal...Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O., Yao, S., Mortrud...

...using an antibody validated for your protein and application of interest or validating an antibody yourself...antibodies. Whenever possible, use validated antibodies or validate them yourself, so you don’t spend ...yourself. But even with a validated antibody, it can still take time for you to get it to work well. Dilutions...

...Epigenetics began as a correlative field in which covalent modifications to DNA or histones, the proteins...downstream of Tet1 to restore the DNA after cytosine removal. Recently, Albert Cheng’s lab developed Casilio-ME...lab has deposited pX330a dCas9-LSD1 for targeted removal of H3K4me1/2 and H3K9me2 histone modifications....Addgene.org Check out Our CRISPR Guide Pages Find Validated gRNAs to Target Your Genomic Locus of Interest...

...combined with the HiBiT tag to provide complementary validation of protein expression and localization. HiBiT-tagged...which lead to cystic fibrosis, one of the most prevalent inherited diseases. The authors investigated the...-dependent response to EGF (Promega, 2025). The value of HiBiT in these experiments was the ability to...References Hall, M. P., Unch, J., Binkowski, B. F., Valley, M. P., Butler, B. L., Wood, M. G., Otto, P., Zimmerman...

...modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only piggyBac™ transposase...stimulate homologous recombination and Footprint-Free™ removal of the selection cassette by excision-only piggyBac...site is left behind in the genome following donor removal, which alters the endogenous DNA sequence and has...nuclease thereby preventing re-cutting. Transposase removal of the cassette later restores the target sequence...

...better the reproducibility of experiments and the validity of conclusions. Antibody engineering also benefits...the next round of mutant library generation. To evaluate improved properties across large mutant libraries...common lab reagent, so maintaining high-quality, validated antibodies with verifiable data is critical, especially...developed by IPI are extensively characterized and validated, with the application data from IPI and other ...

....twitter.com/drNBUdlQco — The Tarling-Vallim Lab (@TarlingVallim) December 19, 2020 In third place, the...shared by Hannah Drury (@druryhannah) The Tarling-Vallim Lab’s first place-winning entry doubled as their...

... the attainment of a bachelor's degree or its equivalent as a minimum (8 U.S. Code § 1184 - Admission ...must possess at least a bachelor's degree or its equivalent and state licensure, if required to practice ...beneficial to the United States For the purposes of evaluating an NIW, USCIS will also focus on the importance...field EB-1Bs historically have a high rate of approvals with USCIS due to the fact that the requirements...

...This time: E. coli genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base ...E. coli genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed...single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex via the...

...signaling pathways controlling properties like growth, survival, and differentiation.” https://www.addgene.org...frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For each of these...screen for] morphological features, migration, or survival in different contexts.” It’s also possible you...

...organizations that share our community-oriented values. Addgenies like the nonprofit company culture as...gain access to new materials and information. Core values drive decisions at Addgene If you’re ever curious...making process at Addgene, just look to our core values. These accurately reflect our culture and outlook...

...have become a popular model organism because their larval stage lends itself well to studies of neuroscience...startled by acoustic, visual or tactile stimuli, larval zebrafish react by performing a distinct C-bend...in a transparent animal: circuit function in the larval zebrafish." Current opinion in neurobiology 23.1...

...binding affinity (Janda et al., 2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions....2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects on Variable...articles/10.3389/fmicb.2016.00022 Torres, M., & Casadevall, A. (2008). The immunoglobulin constant region...

...Once drug candidates are isolated, they can be validated for specificity to their target, or their direct...effectiveness prior to patient treatment. Target validation When a target is known and a drug is developed... to measure the specificity of that target and validate the hit compound. The standard is to test the ...