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We narrowed to 535 results for: val

Showing: 141 - 160 of 535 results

  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...preparations, we have used quantitative PCR to evaluate the physical titer of our preps. We often repeat...1012 genome copies/mL and both results would be valid. This is why the AAV reference is critical. It allows...digital PCR as a tool for titration and integrity evaluation of recombinant adeno-associated viral vectors...tuberculosis-specific DNA by droplet digital PCR for vaccine evaluation in challenged monkeys and TB diagnosis." Emerging...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...penetrate tissue. Because antibodies are large and bivalent, primary and secondary antibodies cannot be incubated...antibodies. Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes ...incubation can be skipped by pre-binding to 1° Monovalent Monoclonal Only anti- rabbit and -mouse versions...Maleimide-labeling occurs when a maleimide group forms a covalent bond with a sulfhydryl group (–SH), such as that...

  3. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...distribution at Addgene are DH5α, NEB stable, ccdb survival, and Pir1. The majority of plasmids contain either...tracking prevents us Addgenies from wasting hours of valuable time searching for samples and keeps us from discarding...efficiency through automation.       More Resource Avaliable at Addgene Get help with plasmid transformation... molecular biology reference pages! Resources Avaliable on the Addgene Blog Read more Inside Addgene Posts...

  4. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...removes a variable from the lengthy process of validating and using an AAV in a specific experimental setup...three generations are regularly requested and a valuable part of our viral vector service. But why are ...selection process to standardize our feedback and evaluation of viral vectors. This process builds on our ...the data when deciding on which viral vectors to validate in their own systems. Browsing the Data Hub before...

  5. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... Antibiotic resistance is defined as microbial survival despite exposure to antibiotics designed to kill...included) are very skilled at the delicate art of survival. Existing virtually ubiquitously in the environment...resources. This competition drives microbes to develop survival mechanisms. Some microbes even make their own ...thus increasing the initial microbe’s chances of survival. This was the whole basis of how penicillin was...

  6. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...everyone to use at ice.synthego.com. We rigorously evaluated the effectiveness of the ICE tool by analyzing...to the observed data using linear regression. R2 Value - When the ICE linear regression is computed during... also computed and reported.  The higher the R2 value, the more confident you can be in the ICE score.... our CRISPR knockout guide design tool, guide validation tool, and guaranteed editing with the CRISPR ...

  7. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...using HR was found to block the acquisition and retrieval of contextual fear memories [5].  In a modified...other regions of the brain may be involved in retrieval of remote contextual memories. This study is an...real-time inhibition), and behavioral (remote memory retrieval) specificity. Optogenetics can be used to more...2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote memories. Cell. 2011;147...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...help of the DNA tuner program) (Latynski and Valentovich 2014). We routinely clone the purified amplicons...gentamicin-resistance (amplified from TP997). We validated these vectors, which we call “pTOX” in a broad...turn subtly blue or magenta. This is particularly valuable when first optimizing conjugation in strains that...PMID: 25800749. PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer program for the construction...

  10. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... in fusion proteins. Since GFP is such a well-validated, all-round good performing probe, it is the first...different acid concentrations. Overviews of the pKa values of different fluorescent protein are available ...remain fluorescent under acidic conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1...Another peptide-based strategy uses an enzyme to covalently label the peptide with a fluorophore. Both systems...

  11. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Biotin is a cofactor that in its active form is covalently attached to the active site of key metabolic ...in cells and is the process by which biotin is covalently bonded to a specific lysine at the active site...the bond between them is one of the strongest noncovalent protein-ligand interactions found in nature. ...in live mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534...

  12. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the challenges... in DMD patient myoblasts. They observed a 13% removal rate of exon 51, which resulted in appropriately...Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research ...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...work begin — validation, validation, validation! (psst… don't forget to submit your validation data to our...the mechanism of entry if known, and, as always, validate the capsid for your specific application and animal...Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O., Yao, S., Mortrud...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...using an antibody validated for your protein and application of interest or validating an antibody yourself...antibodies. Whenever possible, use validated antibodies or validate them yourself, so you don’t spend ...yourself. But even with a validated antibody, it can still take time for you to get it to work well. Dilutions...

  15. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Epigenetics began as a correlative field in which covalent modifications to DNA or histones, the proteins...downstream of Tet1 to restore the DNA after cytosine removal. Recently, Albert Cheng’s lab developed Casilio-ME...lab has deposited pX330a dCas9-LSD1 for targeted removal of H3K4me1/2 and H3K9me2 histone modifications....Addgene.org Check out Our CRISPR Guide Pages Find Validated gRNAs to Target Your Genomic Locus of Interest...

  16. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only piggyBac™ transposase...stimulate homologous recombination and Footprint-Free™ removal of the selection cassette by excision-only piggyBac...site is left behind in the genome following donor removal, which alters the endogenous DNA sequence and has...nuclease thereby preventing re-cutting. Transposase removal of the cassette later restores the target sequence...

  17. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ....twitter.com/drNBUdlQco — The Tarling-Vallim Lab (@TarlingVallim) December 19, 2020 In third place, the...shared by Hannah Drury (@druryhannah) The Tarling-Vallim Lab’s first place-winning entry doubled as their...

  18. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ... the attainment of a bachelor's degree or its equivalent as a minimum (8 U.S. Code § 1184 - Admission ...must possess at least a bachelor's degree or its equivalent and state licensure, if required to practice ...beneficial to the United States For the purposes of evaluating an NIW, USCIS will also focus on the importance...field EB-1Bs historically have a high rate of approvals with USCIS due to the fact that the requirements...

  19. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...This time: E. coli genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base ...E. coli genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed...single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex via the...

  20. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...signaling pathways controlling properties like growth, survival, and differentiation.” https://www.addgene.org...frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For each of these...screen for] morphological features, migration, or survival in different contexts.” It’s also possible you...
Showing: 141 - 160 of 535 results