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We narrowed to 580 results for: vit;

Showing: 141 - 160 of 580 results

  1. Ras Pathway

    Type
    Collection
    ...construction of RAS pathway gene expression vectors for in vitro and in vivo research. The RAS Initiative - at the...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... understand their activity and visualize the effects of alterations to this activity. Learn more about...protein-protein interactions, visualizing promoter activity, and much more. Read on to learn more about GFP...additional co-factors, substrates, or enzymatic activity – it only requires the presence of oxygen during...almost completely required to maintain fluorescent activity; very little truncation is tolerated, however,...

  3. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... RNA targeting system, Cas13, the "collateral" activity of the enzyme can lead to off targets and result...protein, and a guide RNA. The enzyme has dual activities: processing pre-CRISPR RNA into mature CRISPR...:spacer duplex without detectable non-specific activity. Its major advantage is the significantly reduced...thousands new integrases with efficient integration activity in human cells. Moreover, they optimized the specific...engineering, enabling even more efficient integration activity. They now had a system that could integrate genes...

  4. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...transcriptome” studies, in which, for example, the activity of all genes in response to an experimental treatment...engages existing gene regulatory mechanisms to block activity and does not work easily in zebrafish.) Morpholinos... stages and reconstructed the patterns of gene activity and cell divisions that coordinate development...neuroscience investigations. For example, neuronal activity can be tracked in whole brains of swimming, actively...Zebrafish are also amenable to manipulation of brain activity: optogenetic approaches can be used to determine...

  5. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...system, they were able to monitor lacZ activity over 48 hours in vitro without antibiotic selection. In a ...processes and an antitoxin that suppresses the toxin’s activity. The key to these systems is that the toxin is...death. Conversely, CcdA binds to and blocks the activity of CcdB. Applications of ccdB in plasmid cloning...systems consist of two enzymes that have opposing activities, it is not surprising that they also play toxin-antitoxin...

  6. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...few minutes, I just need to split some cells.” Inevitably, this task would take longer than I thought, ... myself flexibility by never placing too many activities too close to one another. Monitoring task progress...) and, at the outset of a task, I outline the activities that are needed to accomplish these goals. Practically... of each day to remind myself of the specific activities that I need to work on. Throughout the day, I...during the times you’ve blocked out for other activities. This is also part of why I do much of my writing...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...workplace is inevitable, but having who can help resolve these conflicts is not inevitable. When you know...operations and keeping our customers happy, but inevitably there will be gray areas that either a) overlap...meet. Most people can’t maintain that level of positivity past the age of five. But it does mean that even...competing, or accommodating, if you find yourself gravitating most frequently towards these approaches, you...

  8. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...for with in vitro display methods such as phage display (Bradbury et al., 2011). In vitro selection avoids...sequences are expressed as scFvs and screened with in vitro assays to select for strong binders. Then the sequences...2011) Beyond natural antibodies: the power of in vitro display technologies. Nat Biotechnol 29:245–254 ...of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced...

  9. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...phage display assays, ubiquitination activity assays, toxin activity, small molecule binding, growth rate...discover unexpected epistatic changes in protein activity. See Natarajan et al for an example of this. ...Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis Protocols, pg. 103-109. PubMed PMID:.... Stephany, and Stanley Fields. “Measuring the Activity of Protein Variants on a Large Scale Using Deep...

  10. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...other proteins, it may still block the function or activity of the tagged protein because of its size. This...There are several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration is the most...brightness, photostability, maturation time, and acid sensitivity of various fluorescent proteins (Botman et al...Determining other aspects of functionality (catalytic activity or interactions) can be challenging. If a fusion...fusion proteins: random insertion of GFP with an in vitro transposition reaction." BMC neuroscience 3.1 (2002...

  11. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...A major limitation of APEX was its low sensitivity. Sensitivity could be increased with higher expression...aggregation. The Ting lab hypothesized that the low sensitivity was due to suboptimal folding or stability. Thus...identified a mutant with significantly increased sensitivity that did not affect the function or efficiency... BirA* molecule that can reassemble to restore activity when fused to two interacting proteins (Schopp...

  12. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...displays enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from... response kinetics and increased brightness, sensitivity, and photostability under two-photon illumination...signaling receptors, but the options for measuring activity of specific RTKs are limited. In a new preprint...plasmids here!   Torres Cabán CC, et al. Tuning the Sensitivity of Genetically Encoded Fluorescent Potassium ...LiLac has improved response size and reduced sensitivity to pH and calcium compared to previous lactate...

  13. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ...the Mushroom Kingdom. Kravitz Lab 2019 Holiday Door is up and running! @LexKravitz pic.twitter.com/J5bjHQo565...TannevanderWal) December 18, 2019   Holiday door The Kravitz Lab went all out to deck their door. What a fun... December 3, 2019 Hope you’ve enjoyed the festivities in the last month!  ...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...concentrations are low, pADH1 displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ...enabling it to bind the promoter and repress pADH1 activity. Researchers have engineered pADH1 variants that...“middle” ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase of yeast...is active. In this way, you can interrogate the activity of uncharacterized promoters. Similarly, placing...

  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...this post: Glutamate indicators with increased sensitivity and tailored deactivation rates Addgene's own...interneurons Glutamate indicators with increased sensitivity and tailored deactivation rates By Mike LacyGlutamate...significantly improved brightness, kinetics, and sensitivity. iGluSnFR4f is well-suited for imaging rapid ... (2025). Glutamate indicators with increased sensitivity and tailored deactivation rates. bioRxiv, 2025.03.20.643984...). Reprogramming site-specific retrotransposon activity to new DNA sites. Nature. doi: https://doi.org...

  16. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...further rounds of RNAi. This process, known as “transitivity,” calls into question any claims about specificity...specificity of a given RNAi technique. While transitivity enhances the potency of RNAi, it can also increase...transgene, followed by a fluorescence readout of RNAi activity. As a plant researcher, the traditional hpRNA ...Mueller WF, Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer... W (2019) Biological plasticity rescues target activity in CRISPR knock outs. Nat Methods 16:1087–1093...

  18. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...agility as a nonprofit resource can prove to be vital during this period of constrained funding. Addgene... even more valuable as labs look to maximize productivity and efficiency with fewer resources. Fostering...community has demonstrated remarkable resilience and creativity in the face of funding challenges. By strengthening...benefit of everyone. We Want to Hear from You We invite researchers to share how these funding changes ...

  19. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming...tracrRNA system is much more potent than the in vitro-transcribed single guide RNA (IVT sgRNA) approach...Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus synthetic cr:tracrRNA...efficiently and serves as a good control for both the activity of CRISPR reagents and ICC antibody specificity...of the crRNA (aim for 40-60% GC content) crRNA activity Local chromatin structure (although we could successfully...

  20. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...enzyme that has been mutated to block its catalytic activity and instead become trapped in a covalent bond ...specificity for their own ligands with minimal cross-reactivity. Other strategies include using engineered ligases...2). If you’re working with purified proteins in vitro, you can even use the same tag for multiple proteins... actin filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...Vivo Covalent TMP-Tag Based on Proximity-Induced Reactivity. ACS Chemical Biology, 4(7), 547–556. https:/...
Showing: 141 - 160 of 580 results