...diversity to the CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post... a splash in the CRISPR field. We feel that the culture of sharing within the CRISPR community is an essential...Addgene Blog Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing ...Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check Out Our CRISPR Guide Pages...This post was updated on Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly...several unique properties that further expand the CRISPR toolbox. We'll cover how Cas13a was identified,...Cas13a. The origins of Cas13a: An RNA cleaving CRISPR nuclease Cas13a was originally identified in 2015...

...additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause obvious germline...directions and obstacles to clinical CRISPR editing Given the success of the CRISPR-mediated exon skipping approach...To bring DMD CRISPR therapy closer to the clinic, much work is still needed. First, CRISPR delivery must... to be treated clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the speed...Resources on Addgene.org Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated...Having seen CRISPR’s success in basic research, researchers are eager to apply it in a clinical setting...setting. CRISPR is often used for animal germline modification, to repair or add in disease-causing mutations...

...Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide View Other CRISPR Blog Posts...more about Cpf1 and check out our CRISPR guide for a refresher on CRISPR/Cas9.  Figure 1: Cpf1 is...over Cas9 Type II CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest...discovery of this type V CRISPR system proved we had a lot more to learn about CRISPR biology. Later work,...called Cas12a. In 2015, Zetsche et al. added to the CRISPR toolbox with their characterization of two Cpf1...is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Figure from Zetsche et al., 2015. ... How was Cpf1 found and tested? Class 2 CRISPR systems, including the type II Cas9-based system, require...

....02364-19 More CRISPR Blog Posts from Addgene: CRISPR 101: Homology Directed Repair CRISPR Protocol for... UNC at Chapel Hill. One advantage to using the CRISPR/Cas system for genome engineering is the fact that...NHEJ is consequently the principal means by which CRISPR/Cas9-introduced breaks are repaired. The following...variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of hundreds of base pairs... Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell...S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted Nonhomologous End-Joining Strategy for...Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale...

... more information on CRISPR/Cas, check out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your...blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...them into the CRISPR sequence. Separate Cas proteins then express and process the CRISPR loci to generate...the challenge - Evolution of anti-CRISPR genes in phages The CRISPR/Cas adaptive immune system seems like...identified that neutralize CRISPR/Cas. Earlier this year, expression of 1 of 5 anti-CRISPR genes were found to...translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between and ...wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712 (2007). CRISPRdb. Date...

...on Your CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled ...highly active and reduce off targets. For CRISPRa and CRISPRi, these considerations are of roughly equal...wrench: Gene activation and inhibition by CRISPRa and CRISPRi Finally, for modulating gene expression at... at the level of transcription – CRISPRa (activation) and CRISPRi (inhibition) technologies – a nuclease-dead...not quite as broad as for knockout via CRISPR cutting. For CRISPRa, it is most-efficacious to target a ~...How to Conduct Genome-Wide CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org... Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to...

...blog post: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems.  Listen to the anti-CRISPR podcast segment... combined CRISPR and optogenetic techniques to develop a variety of photoactivatable CRISPR tools. These...optogenetic manipulation of CRISPR-based systems in animal models. Use Anti-CRISPRs to Turn Off Genome Editing.... Stop light! Photoactive anti-CRISPRs halt Cas9 activity Anti-CRISPR proteins are a highly diverse group...photoactivatable CRISPR strategies Publication System nickname Photoactivatable moiety Cas9 variatn CRISPR protein...Light-Dependent Anti-CRISPR Bubeck et al., 2018 CASANOVA LOV2 from A. Sativa AcrIIA4 anti-CRISPR protein, co-... D (2018) Engineered anti-CRISPR proteins for optogenetic control of CRISPR–Cas9. Nat Methods 15:924–927...

...Addgene.org Visit our CRISPR plasmids and resources page Read our CRISPR guide Find CRISPR plasmids for mammalian...for My CRISPR Experiment? The three years flew by, but since then, scientists have adapted CRISPR nucleases... Read our CRISPR 101 blog posts Find more information about Cas proteins Download the CRISPR 101 eBook...previous blog post The PAM Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity of ...the Cas9 to use in your experiments   The CRISPR drawer. So many options! Comic by Maya Kostman....to Cas9 While Cas9 remains the most widely used CRISPR nuclease, recent work has shown that alternative...to Cas9. As scientists continue to characterize CRISPR nucleases from a variety of sources, it is likely...

... Check out our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources... Guide for Analyzing CRISPR Editing Results with ICE on Synthego’s blog. CRISPR-based genome engineering...Inference of CRISPR Edits) to solve this problem. This tool was initially created to support the CRISPR analysis...successful CRISPR experiments. Jessica is a sci-fi enthusiast, and when she is not guiding CRISPR researchers...Addgene Blog Browse our CRISPR featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility...methods. Still, generating reliable results from CRISPR edit data requires the help of robust software ...to produce quantitative, NGS-quality analysis of CRISPR editing - enabling a ~100-fold reduction in cost...

...how you can make CRISPR mouse model generation easi-er! Generating mice before CRISPR The process of generating... process takes ~8-10 months. With CRISPR (B), you can inject CRISPR components directly into a zygote ...crRNA and tracrRNA) for maximum Easi-CRISPR efficiency. Easi-CRISPR is compatible with standard microinjection...Addgene.org Check out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating Cre-dependent...conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the power of ssDNA donor...