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We narrowed to 138 results for: THY-2

Showing: 81 - 90 of 138 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... pCRISPomyces-2 contains Cas9 and a gRNA cassette. The simpler system of pCRISPomyces-2 displays a higher...and a subsequent curing protocol that requires only 2-3 hours incubation. E. coli and T. citrea scarless...four major components including (1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting ...to dCas9 and efficiently introduce cytosine-to-thymine substitutions in target sequences (Banno et al....

  2. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...C-terminus of human codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to ...material for the bioproduction of therapeutic methylxanthine drugs like theophylline. To facilitate future...enables the degradation of caffeine (1,3,7-trimethylxanthine) and its conversion into xanthine. The majority...constitutive promoter (BBa_J23100) and expresses N-demethylases and other required enzymes, including a glutathione...rescued by the xanthine produced via complete demethylation of caffeine. For example, the Barrick lab was...

  3. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...PMID: 24336571. PubMed Central PMCID: PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... that all look exactly alike.      Figure 2: Reagent gradient. Here, the lanes are lightly marked...guide. The gel is then removed from the membrane (2) and the membrane is cut so each lane is its own piece...in the bands, you may want to consult Tables 1 and 2 in our Technical Design of a Western Blot post to ...the antibody’s dynamic range. Specifically, the 1:2, 1:1, and No dilution samples all have approximately...conditions are 4 °C overnight or room temperature for 1–2 hours, there is some evidence that antibodies may ...incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect...

  5. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...diffusion test or Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results...." London: Wellcome Trust & HM Government (2016). 2. CDC Information Pages on Antibiotics 3. WHO Antimicrobial...flu) Overuse of antibiotics in livestock farming Lengthy courses of treatment with antibiotics These scenarios...

  6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct...nucleotide sequences. To counter this, methyltransferases add methyl groups to bases at specific nucleotide...more stable than the methyltransferase. When a plasmid is lost, the methyltransferase is degraded and/or...composed of restriction endonucleases and methyltransferases. Restriction endonucleases cleave DNA at ...the cell grows resulting in the appearance of unmethylated sites in the chromosome. The more stable endonuclease...

  7. Let There Be LITE Plasmids

    Type
    Blog Post
    ...cryptochrome 2 are the chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally...

  8. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first...operates as an obligate aerobe. It’s also a methylotropic yeast, meaning it can utilize methanol as its...peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii is an excellent model...

  9. CRISPR in the Clinic

    Type
    Blog Post
    ... Cure for HIV/AIDS. Int J Mol Sci. 2023 Jan 13;24(2):1563. doi: 10.3390/ijms24021563 Resources on Addgene.org.... Finally, allogenic therapies involve editing healthy donor-derived cells and delivering them to the ... have been modified ex vivo, previously edited healthy donor cells can be delivered immediately, saving...clinical trial for SCD in 2022 as well. Hereditary transthyretin amyloidosis (hATTR) hATTR is caused by a variety...

  10. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...access to the results of federally-funded research. 2)      In June, the White House honored 13 “Champions... a few minutes to reflect on some of the most noteworthy developments in open science this year. Here ...
Showing: 81 - 90 of 138 results