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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 125 results for: lenti crispr

Showing: 81 - 90 of 125 results

  1. Plan Your Experiment

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    Collection
    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to ...difficult cell types like primary cells. For CRISPR delivery, lentiviral and adeno-associated virus (AAV) vectors... cargo (in this case, CRISPR components) integrates into the genome. Lentiviral vectors are a good option...

  2. CRISPR Pooled gRNA Libraries

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require...Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman OhAinle 3rd 8 15,416 Human CRISPR lncRNA ...18,343 Human UBDUB CRISPR Knockout Library 171531 Knockout Human Li 3rd ~6 9,274 MYC-CRISPR Library 173195... (e.g. primary cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to...

  3. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    Blog Post
    ...Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled screening using sgRNAiBAR. Image from Zhu...'s lab at Peking University has developed a new CRISPR screening method that makes use of guide RNAs with...false-positive rate discovery rate, conventional CRISPR screens require a low multiplicity of infection...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation...

  4. Addgene: The First Twenty Years

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    Blog Post
    ...And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs....org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online course...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing...

  5. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post
    ... to. When it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred...1990s and quickly rose to popularity. Compared to CRISPR, RNAi methods are often quicker and the repositories...vast and difficult to beat in terms of size. Like CRISPR, there is a variety of small RNA types to choose..., it was the go-to choice for many years before CRISPR burst on to the scene. shRNAs reliably knock down...in Plant Biology: New Tools for an Old Favorite CRISPR 101: Targeting RNA with Cas13a (C2c2) Plasmids ...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs...

  6. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post
    ...Resource Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus...to help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols... What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to ...their options for tools such as empty backbones, lentiviral packaging systems, and luciferase expression....a Liquid Bacteria Culture Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty...Addgene blog Twenty Years of Addgene Sharing: CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...

  7. CRISPR Plasmids and Resources

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    Collection
    ...Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and the basics...planning your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've...analysis, discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's... are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout...ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post
    ...development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering...contrast to using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering... et al. "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643...Development of a novel mouse glioma model using lentiviral vectors." Nature medicine 15.1 (2009): 110-116...

  9. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ....cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and transcriptional activation screening...Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction Additional... individually overexpressed and available for lentiviral packaging and delivery to target cells as a pooled... Fig. 1: Barcoded TF library was packaged in lentiviral particles and used to transduce human embryonic...

  10. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown...proteins to monitor the localization of these genes, CRISPR to disrupt their function, and C. elegans overexpression...
Showing: 81 - 90 of 125 results