Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 972 results for: Abo;

Showing: 241 - 270 of 972 results

  1. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...You never know when you will meet a potential collaborator Some of these people you meet will review your...know personally Scientists in your field will know about grants and projects that might help your work Tenure... your card, they have to give you theirs! Think about how you want to introduce yourself and what you ...those you would like to try and meet. Don’t worry about contacting someone much more senior than you – meeting...questions you are pondering and be ready to talk about them. People love to help especially when solving...“Is this seat taken? Can I join you?” Next talk about the food and then move on to science. More great...conference meal etiquette are here. Don’t only talk about science and work – casual socializing is how people...

  2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ... related blog posts Learn about our cancer collection pages Read more about the tumor microenvironment...the tumor. How do microenvironment enzymes and metabolic reprograming influence tumors development? Being... vice versa, let’s take a look at cancer cell metabolism. Cancer cells promote oxidative stress leading...of cells affect tumor progression, enzymes and metabolism reprogramming aren’t the whole story. It’s essential...particularly interested in science journalism and writing about biomedical issues. Follow her on Twitter @BrbaraPinho6...resources page  Find plasmids for your research Learn about Addgene's viral service ...

  3. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...spectrum is located in a range of wavelengths of about 380–700 nm. Photons of lower wavelengths are higher... 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were to use a laser that excites at...optimal excitation happens at higher wavelengths of about 750 nm. Source: https://www.bdbiosciences.com/en-us... we start building your first panel, we'll talk about one last principle of color emission. As you can...our blog post on color compensation to learn more about them. Alright, let's build your first panel! Designing...Conclusion You made it! In this post, you learned about the principles behind fluorophores and their colors...this article again. In the beginning, we talked about the relationship between a photon's wavelength and...

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...potential beneficiaries. Collaborative therapy For the most efficient outcomes, collaborative therapies must be... of the tolerance-producing antigens enunciated above. Finally, signals released from the microenvironment...are in their clinical trial phase. In all of the above therapeutics, techniques like genome engineering...are beginning to see the fruits of many years of labor with up and coming immunotherapy treatments. As ...clinic, physicians and researchers are learning much about how treatment regimens can be improved and how the...ahead of the disease in patients and developing collaborative treatments will go a long way in combating this...Additional Resources on the Addgene Blog Learn About Our Cancer Collection Pages Plasmid Grant to Aid...

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...intended brain region and/or cell type (I’ll talk more about this in detail later). But what are the different...of your opsin, but keep you eyes open for posts about the next steps in the coming weeks. Expression of...Addgene. See the Deisseroth lab homepage for details about types of constructs available, instructions for ...1, 2, 4]. And, of course, the best way to learn about the many different opsin variants and how they can...point.  In brief, some standard opsins (see table above) include Channelrhodopsin2 (Chr2), a blue-light ...construct. Stereotaxic surgery  As mentioned above, AAV opsin constructs are injected directly into...

  6. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...and gives you a sense of what people are excited about. Following scientists in fields outside your main...research organizations, and universities often tweet about publications in the fields of research they support...journals: They publish papers, so naturally they tweet about papers too. You can follow journals related to your...field of research or larger journals that publish about a broad range of topics.  Twitter paper bots: Twitter...number of retweets, likes, and comments on a tweet about a paper can help you gauge how “hot” a paper is....papers that the scientific community is talking about and jumping into the discussion! Semantic Scholar...and search for papers later. Pro tip Learn more about authors by exploring author pages (for example, ...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids...always emphasized the need for open science and collaboration. The field already has comprehensive reference...planned for August 15th, 2019. For more information about the aims and approach of CeNGEN, check out their...elegans. This database provides detailed information about C. elegans 3’UTRs structures, alternative polyadenylation...addition to the wealth of open resources described above, there were many plasmid tools presented at the ...by the worm community. For additional resources about C. elegans and the plasmids we have at Addgene check...

  8. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...heard about it, I got excited from a computer scientist perspective. But, the more I thought about it, ...maintains a list of active spaces near you.   Learning about C. elegans with the OpenWorm Project One such space... an example of one of the problems we work collaboratively to solve, we want to focus our research on ...mutant known as CX51 that exhibits motor defects above 25 degrees. Computer scientists and biologists work... work together to compare the predictions about the effects this mutation has on locomotion in our computer...optimizing the health of domesticated cats and learning about feline microbiomes. One of the aspects of this project...

  9. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...larval stage, Drosophila will pupate, which takes about one week from the initial deposition of the fertilized...there are many resources to help you learn more about fly biology and fly-based experimental systems. ...resources will direct you to the latest information about gene function, while others offer fly stocks and...and other reagents. If you’re ever confused about the best way to study YFG in flies, there is likely an...absolutely essential. You can look up information about your fly gene using the search box in the upper ...In the next post, you’ll learn the fundamentals about how many of these resources work. Many thanks to...Site Specific Recombination in Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila...

  10. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ... elevator pitch slightly if you know a bit more about the other person. If you have a poster or talk at...seemed confused, bored, or particularly excited about? If you tried tweaking your pitch throughout the...conference If there’s a cool new method that you learned about at the talk, bring it to your next lab meeting. ...questions. If you or your lab identifies a potential collaborator from the meeting, reach out to them, pointing...their talk at the meeting and why you think a collaboration would be beneficial. The poster session Before...conference. Networking All of the things we've mentioned above could be considered networking, but here are some...you remember who they are and what you’ve talked about. After the conference Follow up with those that ...

  11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...Foundation's Linda Kahl explains the Addgene collaboration this way: "Addgene is one of the leaders in ... our core values." Addgene spoke with Endy more about the BIOFAB and BIL gates plasmid kits, the Biobricks...reference to George Boole who in 1854 was thinking about logic and analyzing human language and observed ... freely available? Endy: When we start thinking about languages, humans have two types of languages: one...does; it's free to use and that tends to be true about most successful human-to-human languages. Who owns...signals. Let's say I wanted to detect every primary metabolite in a cell. The only way to do that at scale right... what can you use biology to make? If you think about the BIOFAB collection and really dig into its engineering...

  12. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...share. I spend a lot of time telling scientists about Addgene’s repository, and explaining how to deposit..., lab managers, and grad students. We’ve talked about their hot-off-the-presses papers, the awesome new... learned along the way that a connected and collaborative community makes not just for a better day at...technologies and breakthrough research. Insights about how to navigate through all stages of your career...cloning and plasmid-based technologies. Commentary about scientific sharing and the open-access movement....So join the conversation! Do you have a question about culturing iPS cells? Confused by which system to...

  13. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...have been written about CRISPR alone, and Zimmer himself has written entire books about optogenetics. Defining... been thinking about a long time. For the scientists who like to learn everything about one specific topic...wisely makes no such attempt. He is transparent about his own biases--many of which will be shared by ... a massive, expensive project that required collaboration from scientists around the world. The paper ...As a scientist who enjoys learning a little bit about many different things (part of the reason Addgene...

  14. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular...this type of primer does not provide information about the orientation of the insert. Orientation-specific...Orientation-specific primers: If you need information about insert orientation, then you might consider designing orientation-specific...insert Backbone-Specific Provides information about the size of the insert and if it's in the plasmid...plasmid dependent Doesn't provide information about the orientation of the insert Orientation-Specific... before pipetting them onto the gel. The figure above summarizes generalized expected results for the ...

  15. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...noticed Holly Bik live-tweeting about topics I needed to know more about. I caught up with her outside ...me for coffee and spent an hour with me talking about the tools I could use, and some experimental hurdles...other folks at the meeting. I didn’t know anything about these people at the time, but I met some very accomplished... my mind because we’d had discussions in my lab about whether or not to keep DNA sequencing singletons...singletons out, and there is healthy debate online about this (our lab keeps singletons). I felt like anyone...scientific conferences Read our ECR Toolbox blog post about using social media as a scientist ...

  16. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...common cancers like lung (about 224,000 diagnosed this year) or breast cancer (about 246,000 diagnosed this...Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different avenues that... asbestos exposure to the time of diagnosis) is about 35 years, but it usually ranges from 20-50 years... an orphan cancer; it is rare disease with only about 3,000 new mesothelioma patients diagnosed every ...from mesothelioma several years ago – recently collaborated with Senator Barbara Boxer to introduce the ...Additional Resources on the Addgene Blog Find out More About Cancer and Addgene's Cancer Collection Pages Learn...

  17. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ... the world. As an organization that directly collaborates with NIH-funded researchers and utilizes grants...our own initiatives, Addgene is deeply concerned about these developments. These abrupt changes threaten...valuable when budgets are constrained. This collaborative approach eliminates redundant work, saving time...partnerships: We're seeking and expanding our collaborations with industry and public and private partners...challenges. By strengthening our commitment to collaboration and resource sharing, we can navigate this difficult...collective response. Addgene stands ready to collaborate with partners across the research ecosystem to...

  18. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...Institute on Aging in a commentary about the new work published in Cell Metabolism. But, they note, there is plenty...diseases.” I don’t know about you, but I think I could use a good run right about now. And, if you are looking...be important to identify the key proteins and metabolites that are dynamically secreted by muscle during... Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced depression...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...but be sure to let us know if you'd like to write about your plasmids in a future blog post. No time to ...pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long Liu at Oxford University, we are...you plan to use GMAP for your experiments, think about depositing your new plasmids with Addgene to help...and context-dependent human fitness genes, the laboratory of Jason Moffat at the University of Toronto ...effectiveness of their library design, Moffat laboratory members conducted multiple screens in different...This work has been published in Cell. The Moffat laboratory maintains a website for the TKO library that ...selection markers, allowing for use with both laboratory and prototrophic strains. The vectors were based...

  20. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ...her better! How did you first hear about Addgene?  I heard about Addgene from Joanna Brownstein, an Addgene...Additional resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...money for childhood cancer through the sale of art. About two years ago, I had a big role in organizing an...making, reading, sailing, and gardening. Learn more about Julie Cicalese  Ms. Cicalese served as the Chief...

  21. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...variety of short- and long-form written content about timely scientific topics, as well as archived seminar...members gave up a beautiful summer Saturday to learn about science, but they didn’t seem to mind! Attendees...consistently mentioned how pleased they were to learn about these exciting scientific topics at a level accessible...other attendees and getting the chance to hear more about what life is like as a student researcher. The future... with the community? Addgene would love to hear about your efforts to engage the public - let us know ...know in the comments section! More information about SITN: DayCon event website (includes archived content...

  22. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ... Learn about research applications of antibodies in Introduction to Antibodies Learn more about fluorescence...Stay tuned for a future blog post to learn more about these techniques!  Direct vs. indirect immunofluorescence...different approaches to be familiar with when talking about IF, direct and indirect. For direct IF, researchers...immunofluorescence procedure with key considerations about each step. Image created with BioRender.com ...of protocol you will need to use. (To learn more about antibody validation, this commentary by Uhlen, et...Fluorescence Microscopy Techniques is Best for Me? Read about another common antibody application in The Basics...

  23. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... Production Specialist, I’m constantly thinking about ways to improve our productions to deliver the best...four months after I started with Addgene, I was about to have a check-in with my boss Jennifer. I had ... would be a couple months. Jennifer and I spent about an hour that morning scrambling. Everything we had...someone to be comfortable on camera while thinking about things like lighting, camera angle, and shot composition... allowing people to sit without having to worry about getting in close to mic them up. Monitor: I then...first video in our Intro to the Lab Bench series about PPE! We made this with a combination of in-lab filming... Video production, just like science, is always about iteration and improvement. You have an idea, you...

  24. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...Something that I struggle with as I’ve been thinking about my career options is being an overthinker. Being...Stepping into this new virtual world, I was worried about how my ideas would be translated through Zoom calls...Series on Instagram. I interviewed fellow Addgenies about their role in the company. One of the things I wanted...Specialist. Both of these Addgenies really made me think about my future. They made me rethink having a structured...not only in terms of its employees but in general about anyone who steps foot through their door.  In my...just an intern but I was an Addgenie. I thought about all that could go wrong with this internship but...

  25. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...shown in photo), has been speaking out and writing about issues in our science training infrastructure for...some years.  He spoke eloquently and vehemently about the need for change.  Dr. Bourne strongly believes...fact that the PhD and Postdoc advisors only know about academia and alternate training needs to be provided...have to be in academia to make change.  He talked about his own unique way of funding basic research at ...at NEB and got some applause when he talked about how he boycotts publishing in all but open access journals...postdoctoral program.  His strongest comments were about the astounding lack of reproducibility being recognized...

  26. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...be used in people until we learn more about safety and more about the how to use genome edited stem cells...because Jennifer did her graduate work one floor above mine in the lab of Dr. Jack Szostak, who went on...ceremony honored Charpentier and Doudna, who talked about their research leading up to the discovery of the...Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity” driven project. Both...importance of the strong, cross-disciplinary collaboration they shared to the work they did together. They...

  27. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ... outreach and community engagement. Information about contacting Addgene can be found on our Contact page...Europe office located in London and operated in collaboration with LGC Standards. And now you’ll be able to...ll be poised and ready to answer your questions about Addgene, plasmids, and depositing (all while our...carrying out my studies, and getting them from collaborators was extremely helpful, but with hindsight I ... and Australia, viewing science as a global collaboration. This is why she is excited to be working at...This love of interacting with people and talking about science makes her the perfect Addgenie and she can...

  28. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... Delivery Methods Learn about Mouse Work on Exercise and Depression Read About Brainbow Resources on ...success is not always guaranteed. To learn more about this process check out our Mouse Modeling blog posts...by CRISPR. The sequence started clean and then, about 100 bps into the run, it got “dirty”, with multiple...makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease...by Southern blot. From the efficiency discussed above extends 4 major advantages of CRISPR mouse editing...targeting. It also costs less. With CRISPR, it may cost about $5,000 to generate founder mice, while with conventional...

  29. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ... post if you debating about an academic or non-academic career Learn more about alternative science careers... written for selfish reasons: I wanted to learn about careers in science communication. When I started...Communication Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest...option. So if you’re interested in learning more about science communication careers, you’re in the right...careers here Resources on Addgene.org Learn more about careers at Addgene ...

  30. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...Additional Resources on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene...the American Cancer Society, or maybe you think about a local food pantry or community arts organization...community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is strength ...accelerate research with easier sharing and collaboration. Joanne and all the Addgenies: Adam, Amanda ...Connie Cepko Resources on Addgene.org Learn More About Our Mission Meet the Addgene Leadership Team Get...
Showing: 241 - 270 of 972 results