We narrowed to 339 results for: ADA
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TypeBlog Post...reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of concept...Gaudelli et al., 2017). GO is thus a flexible and adaptable tool to identify and enrich for base-editing events...
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Bioinformatics at Addgene
TypeBlog Post...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source... -
Transferable Skills Guide: Creativity
TypeBlog Post...your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford conducted... with the group (because they will fight more adamantly for their solution), selecting a devil’s advocate... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...support entry of required metadata, with the option of adding additional metadata in the repository after... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...While selfish in nature, these systems have been adapted by researchers for many uses when plasmid retention... PMID: 8650219. PubMed Central PMCID: PMC39188. Kamada, Katsuhiko, Fumio Hanaoka, and Stephen K. Burley... -
Hot Plasmids - November 2023
TypeBlog Post...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...such that prior formats are no longer supported/readable. Is the author or their host institution expected...Research Institute at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine kinases... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray, 2010. These episomes...Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, S., Thornhill, S. I., Parsley, K. L., Staal, F.... -
Hot Plasmids: Summer 2024
TypeBlog Post...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...twist since it relies on the sequential nicking, degradation, and PCR-generation of a mutant version of the...Natarajan, Chandrasekhar, et al. "Epistasis among adaptive mutations in deer mouse hemoglobin." Science 340.6138... -
Hot Plasmids: Spring 2025
TypeBlog Post...Rising τ, and Decay τ of the three sensors. Image adapted from Aggarwal et al. 2025 under a CC-BY-NC-ND 4.0...displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et al. 2024 bioRxiv... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...straight-forward, concise explanation of how to adapt our engineered devices, recombinase-based state ...not expressed in each state. To help researchers adapt GRSMs for their research, we provide MATLAB-based... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...you are working with RNAs, which are prone to degradation by the omnipresent RNase. You may want to declare...doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD: A Novel... -
Finding Your Perfect Job After University
TypeBlog Post... culture, and also needed to be resourceful and adapt. By the end of my three months, I fully understood...companies which have more bureaucracy and cannot be as adaptive. I was also surprised to learn that I enjoy ... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post... editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017, the Korswagen lab...doi.org/10.17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013)... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...Lavis Lab (Grimm et al., 2017). These dyes were adapted into ligands for HaloTag® and are currently offered...doi.org/10.1128/spectrum.03690-23 Mahmoudian, J., Hadavi, R., Jeddi-Tehrani, M., Mahmoudi, A. R., Bayat,... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...functions more similarly to CRISPR/Cas9 systems adapted for eukaryotes in that Cas9-mediated cleavage induces...addition of a uracil DNA glycosylase inhibitor and degradation tag on the fusion protein to limit the activity... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post... the balance of biogenesis and mitochondrial degradation in vitro and in vivo. Merged green and red ...long life-cycle of a protein from expression to degradation. Chemical approaches may require expensive peptides... -
New Coomassie Protocol Video
TypeBlog Post...Antibodies." Hot tip! This protocol can easily be adapted for other types of proteins. If this video ... -
CRISPR 101 eBook...en español
TypeBlog Post...está disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos...