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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 551 results for: ADA

Showing: 161 - 180 of 551 results

  2. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016. Until recently...

  3. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...longevity in eukaryotes and promote transcript degradation in prokaryotes.  Prokaryotic termination   Prokaryotic...important for stability of the mRNA, protection from degradation, and is integral to the nuclear export and translation...

  4. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...References and Resources References Braun, C. J., Adames, A. C., Saur, D., & Rad, R. (2022). Tutorial: design...Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J., & Kampmann...

  5. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic tools, they’re optimistic...optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing science...

  6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...for using CRISPRi in many types of bacteria: lab-adapted, environmental isolated, and disease-associated...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...the kinetic response time from signal input to readable output, which generally exceeds that of the VSD...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... co-purify with your plasmid DNA and lead to degradation of your prep. Many plasmid prep kits recommend...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...

  10. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...blog posts and additional resources into one downloadable PDF that you can save on your desktop. The Plasmids...

  11. Typing CRISPR Systems

    Type
    Blog Post
    ... all CRISPR types; the fast evolution of these adaptive bacterial and archaeal immune systems has led ...suspected that they don’t carry out the same types of adaptive immune functions that other CRISPR types do, due...resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2, which are critical for ...pyogenes) belonging in II-A. Cas9 was the first to be adapted for use in mammalian systems, with the landmark... become the most engineered Cas enzyme, with adaptations for increased fidelity, activation/repression...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...protocols, visit https://clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com...New World Monkeys and rodents. Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a...doi.org/10.1038/nn.4593 Chen, X., Ravindra Kumar, S., Adams, C. D., Yang, D., Wang, T., Wolfe, D. A., Arokiaraj...Zheng, Q., Beddow, T., Zhao, B., Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg,...Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai, H. (2018). Intravenous administration...Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw...Widrick, J. J., Messemer, K. A., Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz...

  14. An Introduction to Adenovirus

    Type
    Blog Post
    ...cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes..., J. S., Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., Rizzo, K., Bertumen, J. B., Morales...

  15. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated..., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C. M., Leib, R. D., Cirolia, G., Thomas, D., Stamnes...

  16. DNA Purification Without a Kit

    Type
    Blog Post
    ...or TE. The Bishop Lab’s glass milk protocol is adapted from the 1979 Patterson Protocol. When published...100 bp to 48 kb with high yields and without degradation during the extraction procedure. Additionally...

  17. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...resistance to both carb and amp, due to their common degradation mechanism. How to choose between the two? Carb...high pH environments and is less susceptible to degradation in agar plates. This prevents satellite colonies...

  18. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...product, or chromosome. One of the main causes of degradation during plasmid DNA extraction and purification...protein endonuclease I encoded by the gene endA. Nowadays, the most common E. coli strains used for plasmid... extra steps like heat inactivation, protein degradation, and/or the use of chelating agents that can ...store your sample without having substantial degradation and which experiments you can use your plasmid...

  19. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...relevant metadata and the screen results. Screenshots updated 2024. Importantly, metadata for each...gene hits for each screen are also captured as metadata.   Screen results are reported as the original... diversify, the BioGRID ORCS resource will be adaptable to a wide variety of screen formats and contexts...
Showing: 161 - 180 of 551 results