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Showing: 161 - 180 of 382 results

  1. TALEN Guide

    Type
    Collection
    ...from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that...

  4. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant...

  5. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

  7. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized... and results were consistent with copy number variation (CNV) analysis of the U2OS cells.   Figure...

  8. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...typically have a wide range of input quantities. This variation is problematic for conventional NGS library prep...range (1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and...

  9. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for...

  10. Control AAV Preps

    Type
    Collection
    ...Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None...

  11. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...right for me? Here’s a list of some important variables to consider when choosing an inducible promoter...cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic ...

  13. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...

  15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  16. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ..., large collections of biological independent variables, are becoming increasingly popular tools with ...your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...

  18. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...condition that can apply enough pressure to select for variants of interest, though. A selection can take the ...
Showing: 161 - 180 of 382 results