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We narrowed to 391 results for: ARIA

Showing: 161 - 180 of 391 results
  1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism...
  2. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...
  3. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant...
  4. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...
  5. TALEN Plasmids and Kits

    Type
    Collection
    ...GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena Torres-Padilla These plasmids were designed...
  6. Control AAV Preps

    Type
    Collection
    ...Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None...
  7. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for...
  8. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...typically have a wide range of input quantities. This variation is problematic for conventional NGS library prep...range (1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and...
  9. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized... and results were consistent with copy number variation (CNV) analysis of the U2OS cells.   Figure...
  10. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...Japanese firefly (Luciola cruciata), and more. These variant proteins offer different kinetics, substrate requirements...measurements of luminescence from live cells. Luciferase variants with incompatible substrates can also allow multiplexing...
  11. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...
  12. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...
  13. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ..., large collections of biological independent variables, are becoming increasingly popular tools with ...your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...
  14. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...
  15. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...right for me? Here’s a list of some important variables to consider when choosing an inducible promoter...cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic ...
Showing: 161 - 180 of 391 results