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Showing: 161 - 180 of 383 results

  1. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized... and results were consistent with copy number variation (CNV) analysis of the U2OS cells.   Figure...

  2. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for...

  3. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...typically have a wide range of input quantities. This variation is problematic for conventional NGS library prep...range (1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and...

  4. Antibody Production

    Type
    Collection
    ...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...

  5. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...right for me? Here’s a list of some important variables to consider when choosing an inducible promoter...cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic ...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  7. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ..., large collections of biological independent variables, are becoming increasingly popular tools with ...your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...

  9. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...condition that can apply enough pressure to select for variants of interest, though. A selection can take the ...

  12. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...

  15. Antibody Plasmid Collection

    Type
    Collection
    ... in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector system for isolation...

  16. mTOR Pathway

    Type
    Collection
    ...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream...

  17. RNA Extraction Without A Kit

    Type
    Blog Post
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

  19. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...

  20. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...
Showing: 161 - 180 of 383 results