We narrowed to 217 results for: Apple fluorescent protein

...required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic acid targets.  While...stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus ...Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and... to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting... cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR applications where limited... this method for in vivo applications. This technique utilizes Cas9 proteins harboring receptor ligands...delivery of, at minimum, two components: the Cas9 protein and a guide RNA (gRNA) targeting your genomic site...

...Proximity Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications in Cells and Tissues...the antibody sequence or that rely on strong protein-protein interactions with other peptides, like an Fc-binding...non-canonical amino acids or peptide tags. 5) Strong protein-protein interactions with other peptides. Created with...detect your antibodies using techniques like fluorescence microscopy, flow cytometry, or spectrophotometry...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...

...plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya...Scientists can also use electroporation, the application of an electrical charge to cells, to increase...

...interest is expressed together with a marker (Fluorescent protein or selection marker) allowing for selection...delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector background Until ... psi signal, the genes encoding the structural proteins could not be integrated into the new viral particles...plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the stability and broadens... one with the exception that the HIV accessory proteins that are not essential for the production of the...dividing and non-dividing cells No immunogenic proteins generated Stable integration into host genome...have been increasingly popular in fundamental and applied research since their first use in the early 90’...

... CRISPR Protein Tagging CRISPR Plasmids - Protein Tagging Browse CRISPR Plasmids...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and ...134.7 KB Mendenhall and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid...on endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to ...2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...

...AAV, work has focused on modifying the outside protein shell, or capsid, of the virus so that it only ...optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV...promoter to drive expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only...reproducibly labeled retinal cells can be found here.  Applications for synthetic promoter AAVs Besides restricting...synthetic promoters have a few other potential applications: Using promoter combinations for targeted expression... promoters expressed in. The lab tested this application using one promoter to drive expression of Cre-mCherry... rates of labeling are ideal of gene therapy applications, promoters that produce sparse labeling would...

... are trying to remove from your sample is protein. Proteins absorb light to varying degrees across the...specific to proteins and why researchers traditionally use the A260/280 ratio as an indicator of protein contamination...downstream experiments. The usual suspects, such as proteins, phenol, or guanidine salts, exhibit their own...how different contaminants impact these ratios.  Protein contaminants Whether you are purifying plasmid ... The A260/230 ratio can be a good indicator of protein contamination, but a number of other potential ...A260/280 ratio does not drop significantly until protein levels exceed 70% of the sample content (Loughrey...A260/280 ratios could still have quite a bit of protein present.  Purification reagent contaminants The...

...genes through several different applications such as fluorescent protein fusion studies and the generation...suppressors, and their protein products. Proto-oncogenes consist of a group of genes/proteins that, when expressed...refers to 3 proteins (H-Ras, K-Ras, and N-Ras) encoded by a family of proto-oncogenes. Ras proteins are special...not ideal environments for many cell types. From proteins to pathways - Ras as a model When mutations do...different cancers and identifying the roles of key proteins involved, with the goal of being able to discriminately... of Ras mutations in a variety of cancers, Ras proteins have stumped the pharmaceutical industry for years...lack of “druggable” domains. In other words, Ras proteins naturally lack areas that are accessible, high-affinity...

...vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully). This next...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...deletion from the N terminus of the wild-type TALE protein (previously shown to preserve the DNA-binding ability...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning...length human LSD1 at the carboxy-terminus of the protein. 49043 EMM67 49044 EMM71T Golden Gate TALEN 2.0...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...

...present that vary in cell type, size, and marker proteins. But even if you are analyzing a homogenous cell...laser impacts the magnitude of FSC, SSC, and all fluorescent stains. The cytometer's software will allow you... my analysis. For this measurement, I used a fluorescent dye that can only enter dead cells, while living...interested in measuring a population based on a fluorescent stain, you might be wondering which second measurement...voltage describes an electric potential that can be applied to the photomultiplier inside the cytometer to ...

...Biotin protein ligase (BPL) is responsible for this highly specific post-translational protein modification...the lab are protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes...terminus of the protein. BirA has been shown to effectively biotinylate AviTag expressing proteins in bacteria...fusion proteins Learn how biotin is used in Split-BioID: An improved method for studying protein-protein...about in vivo biotinylation of bacterial fusion proteins Biotin is an essential vitamin for life Although... general process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically...entire organism on the biotin carboxyl carrier protein (BCCP). Discovery of the biotin-avidin interaction...

...interacting with proteins such as transcription factors and chromatin modifiers. These proteins recognize and... a minimal promoter to drive expression of a fluorescent gene and packaged into viral vectors that can... To evaluate the resulting expression of the fluorescent reporter, the brain tissue was extracted, sectioned...when discussing raising children, it can also be applied to research. Sharing knowledge and tools is the...

...combinations of protein tags (His, Avi, and GFP) as well as protease sites to facilitate protein purification...the system Easing the Protein Purification Process with pCri Cell-free protein expression kit Cell-free.... The Cell-Free Protein Expression Kit has been used to produced recombinant proteins for in-vitro assays...binding proteins in as little as 3 days. Abil et al., 2014 show that you can fuse custom PUF proteins to a...generate PUF proteins with a wide variety of functions via different fusions. Some applications of PUF protein...Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression Kit FREQ Seq PUF Assembly Kit Below...of interest into both sequencing (pInitial) and protein production (a variety of pExpression vectors) plasmids...

...zebrafish behavioral studies Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series...blood cells or neurons can be fluorescently labeled, allowing researchers to observe changes to these tissues...DNA into the genome is useful for a variety of applications. For example, specific tissues such as blood...pathways control this process. Additional zebrafish applications and conclusions Zebrafish are also popular outside...question, and zebrafish are not ideal for every application. Disadvantages include their duplicated (i.e....—have proven useful in a variety of research applications. Lineage tracing studies highlight how these...Contemporary zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments." Methods...

...of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell... vague and "what proteins regulate the Z pathway?" is an open-ended list of proteins. Instead, consider...consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it easier for the search.... Given this large sum, one might expect that applicants would work for months to hone a chalk talk. Yet...of common questions, check out this eBook about applying for faculty positions. You need to practice with...

...introduce unnatural head groups for applications like fluorescent labeling via click chemistry.    Figure...photoswitchable elements into many other types of proteins. Here, we’ll highlight a few recently-deposited...license. A naturally occurring microtubule-severing protein, Katanin is of course named for the samurai sword...optogenetic module to activate or recruit your protein of interest, maybe you want to inhibit it. Restricting...development and a knockout is not feasible or when the protein of interest supports a variety of processes in ...CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest and express the other separately, then...generate CRY2-CIB1 oligomers that trap the fusion protein away from its usual location, preventing it from...

...Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous... stemloop 2 The MS2-P65-HSF1 activation helper protein Full references are below. Pooled genome-wide human...This plasmid enables Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition...plasmid facilitates Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

... be used as a proxy measure for differences in protein expression. (If you're just getting started, check...fluorogenic primers Low Single self-quenching fluorescent molecule connected to a primer  No Yes ...normalize for that. Passive reference dyes are inert fluorescent dyes that are not dependent on amplification ... It is possible to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to ... Tip🔥 The ABI machines automatically record fluorescent data on all channels - helpful if you forget ...understanding and technical proficiency with common applications - now let’s dive right in! You know qPCR is...as it solves, so understanding and using the application correctly is imperative. Picking your chemistry...

...pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways. Often a FP is fused to a protein of ...bioluminescence. Luciferases usually have shorter mRNA and protein half-lives then GUS and are therefore good temporal...elucidate the subcellular localization of your protein of interest. Find all plasmids from the Lee-Parsons...plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-50. PubMed...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration...

...blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors... though not all, of these defenses. For some applications of genome engineering - such as certain forms...