We narrowed to 960 results for: Cre

...Mammalian Expression HcRed1 588 618 0.3 Dimer pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian Expression...database for fluorescent proteins created by Talley Lambert Credits Contributing Authors Written and reviewed...plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest. Plasmid...Expression HcRed1-C1 - Mammalian Expression dKatushka 588 635 22 5.5 Dimer dKatushka-pBAD - Bacterial Expression...

... plasmid kits and collections are designed for creating plant expression vectors. ID Kit Description Cloning... binary vectors — High-performance vectors for creation of fusion constructs in transgenic analysis of... in just two simple cloning steps. CRISPR Kate Creasey Krainer , Vladimir Nekrasov , Nicola Patron Additional... to understand how scientific publications are created. Papers from the plant biology literature are discussed...growing resource for the plant science community. Credits Contributing Authors Written and reviewed by the...

...blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons function... et al. Cell. 2021) Conduct multimodal genetic screens in human iPSC-derived neurons with a CRISPRi-based...antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq...community. This collection highlights plasmids created with support from the BRAIN Initiative. Jackson...Link opens in a new window) . This resource was created with support from the Chan Zuckerberg Initiative...

...carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that creating and maintaining... accelerate scientific discovery that will help create new and more effective therapies for these tumors...States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

...Scientific Curation team at Addgene. Media Credits Figures created with BioRender (Link opens in a new window...Collection . Return to top Membrane Trafficking The secretory pathway is responsible for delivering proteins...mNeonGreen Dorus Gadella 212022 pcDNA3/td5StayGold(c4)=GianCreg Golgi apparatus Giantin td5StayGold Atsushi Miyawaki... Collection Addgene Blog: Fluorescent Proteins Credits Contributing Authors Written and reviewed by the...

...next generation architecture has been shown to increase mutation induction when using TALENs. The FokI...Gersbach These new destination vectors can be used to create TALE-transcription factors and are used at the ...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation...

...Scientific Curation team at Addgene. Media Credits Figures created with BioRender (Link opens in a new window...cells. Many of these sets have been mutated and screened through directed evolution to charge the tRNA ...to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA All ...window) PMID: 34196546 (Link opens in a new window) Credits Contributing Authors Written and reviewed by the...

... Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene...mTORC1 promotes protein and lipid synthesis, and increases energy production through glycolysis to fuel these... Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini & Kris Wood) References...

...expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...topic. Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants...

...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species...

...plasmids and learn more about the scientists who have created and shared these plasmids....of this shared resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute...

...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com. Browse, sort, or search the...

... modifiers include: P300 — activation through increased H3K27 acetylation TET1 — activation through cytosine...Overview of epigenetic modification using CRISPR. Created with BioRender.com. Browse, sort, or search the...

...breaks in DNA instead of the double-strand breaks created by wild-type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...

...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and...

...CRISPR plasmids that create double strand breaks in DNA.... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...

...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the...

...al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast codon-optimized...window) PMID: 24161108 (Link opens in a new window) Credits Contributing Authors Written by Dennis Botman in...

...paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge the Principal...Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods...

...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...