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We narrowed to 957 results for: Cre

Showing: 161 - 180 of 957 results

  1. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...temperatures, but you may need to increase your primer length and increase the Tm if you are trying to clone...Bacterial Transformation Summary PCR based cloning is incredibly versatile and allows for nearly any piece of ... 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert...

  2. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive...converted to thymidine through base excision repair, creating a C to T change (or a G to A on the opposite strand..., which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases...deaminases do not exist in nature, but have been created by directed evolution of the Escherichia coli TadA...evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both types of base editors...adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase inhibitor...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...In a CRISPR screening experiment, target cells are treated with the pooled library to create a population...mutant cells that are then screened for a phenotype of interest. Screening experiments using a pooled ...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...Blog Posts CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing...libraries and keep the following in mind: CRISPR screening experiments require electroporation to amplify...NGS) to confirm library representation. CRISPR screening experiments require large quantities of cells ...

  4. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with work directed towards increasing on-target editing rates and decreasing off-target editing rates. ...others. Homology-directed repair (HDR) allows us to create a variety of modifications in endogenous loci with... locus makes it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify ...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology... : Base editors directly convert bases without creating a double-strand break. This method displays higher...method targets RNA rather than DNA, it does not create permanent genome edits or double-strand breaks....epigenetic modifiers can modulate transcriptions and create heritable epigenetic marks. Visualize : Fluorescently...

  5. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming of adult pancreatic exocrine cells to beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells...human cells in 2006 and 2007, respectively. To create iPSCs, fully-differentiated adult somatic cells...Plasmids in the table below. Once iPSCs have been created they can be directly differentiated into specific..., and transdifferentiation utilize plasmids to increase expression of key factors. The tables below will...plasmids available from Addgene that can be used to create iPSCs or another cell type. You can also search...iNDI) Collection for plasmids that can be used to create cell lines with endogenously-tagged gene variants...Plasmids Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). Sort the...

  6. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed evolution...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

  7. Antibody Guide

    Type
    Collection
    ... cell cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...animals immunized as described above are used to create hybridomas, which produce large amounts of a single...recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma...goes through a randomized reorganization process, creating a unique binding site. This process, known as ...roles in the immune response, and can be used to increase flexibility or specificity in antibody-based methods... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

  8. Protocol - How to Streak a Plate

    Type
    Protocol
    ...of the plate, as shown in the diagram above, to create streak #1. Pro-Tips Hold your tooth pick at an ...bacteria over a second section of the plate, to create streak #2. Using a third sterile pipette tip, toothpick...bacteria over the last section of the plate, to create streak #3. Incubate plate with newly plated bacteria...

  9. Weighing Reagents Protocol

    Type
    Protocol
    ...using weighing paper, fold it in half first to create a crease and then place it unfolded onto the balance... sure you’re weighing all reagents precisely to create the solution you expect. Understanding how to obtain...

  10. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

  11. Distribution to Industry

    Type
    Collection
    ...Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's Disease.... How to Order Creating an Account: Before placing your order, you will need to create an account and ...for cloning and assembling standardized parts to create eukaryotic multigene constructs. Pooled Libraries... our website. Placing an Order: After you have created an account, you can place orders through our website...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression HcRed1 588 618 0.3 Dimer pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian Expression...backbones with different fluorescent tags for you to create fusion proteins with your gene of interest. Special...backbones with different fluorescent tags for you to create fusion proteins with your gene of interest. These...: A database for users of fluorescent proteins created by Talley Lambert at Harvard Medical School More...Expression HcRed1-C1 - Mammalian Expression dKatushka 588 635 22 5.5 Dimer dKatushka-pBAD - Bacterial Expression... Center at UCSF / QB3 Wiki FPbase : A database created by Talley Lambert at Harvard Medical School Zeiss...

  13. Antibody Plasmid Collection

    Type
    Collection
    ...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...smaller fragment of an antibody, which could offer increased stability and ease of production due to their ...highlights plasmid collections that can be used to create and express plasmid-based antibodies. Description...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...

  14. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

  15. CRISPR Plasmids - Plants

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

  16. Colony Formation Titering Assay

    Type
    Protocol
    ...likelihood of multiple integration events decreases as the dilution increases. Calculate the transduction units... remove cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing...

  17. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...window) . TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...

  18. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins from...Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...tetracycline-controlled transactivator (tTA) was developed. tTA was created by fusing tetR with the C-terminal domain of VP16...tTA binding to the tetO sequences and subsequent increase in expression by the activation domain, resulting...repressor, or rTetR, which reversed the phenotype and created a reliance on the presence of tetracycline for ... etracycline-controlled t rans a ctivator) was created by fusing rTetR with VP16. The tetracycline on ...interest with Strep-Tag and Tet-On 3G transactivator, creating an auto-regulated Tet-On 3G System. Contains homology...
Showing: 161 - 180 of 957 results