We narrowed to 214 results for: DAP

1. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   ...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...

2. Plasmids 101: Optimizing Plasmid Yields

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   Blog Post

   ...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...

3. Plasmids 101: Multicistronic Vectors

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   Blog Post

   ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   ...medicine. The parts we have right now are well adapted to work in prokaryotic bacteria, not in fungal ...

5. Kit Free RNA Extraction

   Type

   Protocol

   ...Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in a new window) Chomczynski P...

6. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ..., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing (Cox et al., 2017...

7. DNA Purification Without a Kit

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   Blog Post

   ...or TE. The Bishop Lab’s glass milk protocol is adapted from the 1979 Patterson Protocol. When published...

8. A Guide to Starting Your Own Journal Club

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   Blog Post

   ...group and topic. For example, it may be useful to adapt the second version (multiple presenter, figure by...

9. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ...formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where...

10. An Introduction to Adenovirus

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    Blog Post

    ...cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes...

11. Getting to Know Expansion Microscopy

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    Blog Post

    ...model systems, paired with SRM techniques, and adapted for detecting nucleic acids as well as proteins...

12. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ...Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus Candida...

13. An Inside Look into Addgene's Dev Team

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    Blog Post

    ...in iterative chunks, we have the flexibility to adapt and easily respond to changing needs of the teams...

14. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...nucleotide-based ligand binders would prove to be highly adaptable tools. The key to aptamers’ flexibility is their...

15. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    Blog Post

    ...Selection, prep, and testing of AAV enhancers in mice. Adapted from the Allen Institute's Genetic Tools Atlas....

16. Hot Plasmids: Fall 2024

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    Blog Post

    ...ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova et al., 2024 under CC BY license....

17. Antibodies 101: The Four ELISAs and When to Use Them

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    Blog Post

    ...measure antibody levels, scientists have since adapted it for a host of different proteins and small molecules...

18. Plasmids 101: Stringent Regulation of Replication

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    Blog Post

    ...Figure 2: Prokaryotic chromosomal replication. Adapted from BioRender template. Since DnaA binding...

19. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...contains structural elements that are versatile and adaptable. Variation in ori structure allows plasmids to...

20. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ...While selfish in nature, these systems have been adapted by researchers for many uses when plasmid retention...