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We narrowed to 824 results for: EED

Showing: 161 - 180 of 824 results

  1. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...Fluorescent proteins are very stable in SeeDB2. However, SeeDB2 is not powerful enough for large brain ...2014), before clearing with SeeDB2. For more information, visit our SeeDB Resources website. We update...proteins were best imaged after tissue clearing with SeeDB2 (Ke et al., 2016, 2018).   How to use Tetbow for... for High-resolution fluorescence imaging using SeeDB2." Bio-Protocol 8 (2018): e3046. Livet, Jean, et...Ultrafast tissue staining with chemical tags." Proceedings of the National Academy of Sciences111.36 (2014...

  2. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...classmates! Your mentors don’t need to be more senior or more “important”, they just need to know you well enough...you are more desperate or needy so you can’t wait until you imminently need a job. Start now. Informational... group We don’t all get the mentoring support we need, and most of us could use more mentors to listen...any science career, there will be a time when you need to speak publicly to communicate your work. One ...career options. 5. Write a grant - even if you don’t need one A lot of science in academia and not in academia...

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... into the PX330 vector backbone? A2: There is no need to add the NGG PAM sequence. Because the 'NGG' of...the PAM is used to select your genomic target, you need to make sure the NGG immediately follows your target...should order is:5'-CACCGN...N-3'3'-CN...NCAAA-5'You need to add the CACC and AAAC cloning overhang. Q3: When...starts with a 'G', you naturally have one and do not need to add an additional 'G'. Q4: Where are double strand...be sufficient to generate off-target DSBs; there needs to be extensive homology of the protospacer followed...your cells. If you want to use double nickase, you need to have four spacers co-expressed and using PX335...the double nickase system, with PX335 vector, you need to find two guides for each cleavage site, but this...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...individual target species. Indeed, in order to use MAGE, the λ Red recombinase enzymes need to be expressed and... easier for you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below... kit Synthetic and basic biologists alike often need to test multiple expression systems before they ...digest and Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily...native methyl-directed mismatch repair (MMR) system needs to be repressed in the host strain. To address this...variety of new compounds being generated, there is a need for genetic optimization of industrial yeast strains...supplied as annealed synthetic oligos - eliminating the need for PCR or additional molecular cloning steps during...

  5. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...polymerize, allowing the slower exonuclease activity to proceed. By including only one type of dNTP in the reaction... can decide exactly how far the T4 exonuclease proceeds: at the first occurrence of the chosen nucleotide...if those overhangs perfectly match up. They just need enough complementary ssDNA to hold the plasmid together...ways. One option, imprecise T4 digestion (no dNTPs needed), allows both insert and vector to be digested ...incomplete PCR — although higher DNA concentrations are needed for this method to be successful. Low DNA concentration...approaches require higher concentrations of insert to succeed. 5′ overhangs are generated in the linearized vector... you can assemble multiple fragments without the need for specific restriction sites or DNA ligase! This...

  6. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...external stakeholders to learn more about their needs and any problems that they are trying to solve. ...test the solutions with the users, gather more feedback, and start the cycle over again to refine the ...software possible. Similarly, when the developers needed to know information from the lab folks, it was ...with our software or devices, and anything they may need to help improve their work. Then, I distill and ... well as the developers. Lastly, you’re going to need to be a problem-solver. A lot of the time, the problem...clear, but the solution is more complicated. You’ll need to be creative to be able to work with others to...strong relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...

  7. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...answer the question, you need only to take that step. Repeat that as often as needed until you get to the ...calm: “Excuse me, I seem to have lost my place. I need a minute.” I then pulled out my notebook, opened...presentation will be better if you take time when you need it, rather than trying to push through a state of... deck gets uploaded. Your mic emits screeching feedback. You’re muted on Zoom. Your video conferencing...telling yourself, “I can stop and check my notes if I need to,” or “It is okay if it doesn’t go perfectly,”...group of peers who are there to provide critical feedback, often with a semi-international audience or not...

  8. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...it off? Turn it down, but not forever? You don’t need to resort to the force to control protein expression...off, tunable, and/or inducible? How long do you need control? Will temporary control (a few days) be ...interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation that will permanently...permanently ablate protein expression. If you need the ability to control when you turn off expression ...you would like. Check out our promoter blog if you need help choosing. Viral expression is another mechanism...exogenously introduced proteins, as many of them just need a special promoter or tag prior to introduction ...endogenously expressed protein, then these systems need to be site-specifically knocked in. This can be ...

  9. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...yeast cells. Need more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs As you probably...read and then come back here once you are up to speed on the basics of the technology. In this blog, we...benefit from a larger library of parts but don’t need to add tags to your proteins, then the CIDAR Kit...a MoClo kit plus the plant-specific parts you‘ll need, including sub-cellular localization signals, plant...It is important to understand your experimental needs before choosing one or more of these kits, as there...specifically for the types of expression and integration needed in mammalian cell types, and comes with a large... to select an available kit that best fits your needs and then modify it as necessary. And remember, if...

  10. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ... style according to the needs of my committee members. Some members would need much more guidance, direction... scholars should have the skills and techniques needed to facilitate effective teamwork, manage day to...sticking to deadlines and performing the work I agreed upon (just as I would in my laboratory work). Committee...would act more independently and some others would need regular re-direction and alignment with the overall...responsibilities Being a leader in an organization, you need to make sure the organization speaks in “one voice... you can find opportunities around you. You just need to be prepared to take on the challenges and be ...

  11. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...then need to be purified by density gradient centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge...cells for further processing. What component(s) you need to collect will depend on the serotype, as some ...however, you’ve collected the producer cells, you’ll need to lyse the cells to let the virions out. We recommend...exchange and further concentrate the virions if needed. For more details on the production process, check...produced, harvested, and purified your rAAV, you’ll need to determine the titer of your viral vector solution...).  To choose the optimal titering assay, you’ll need to understand how different titers are measured....

  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...increase plasmid yield. Keep in mind that you might need to use multiple techniques for a particularly stubborn...bacterial strain to see if additional steps are needed to optimize yield. You might want to include an... high levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein...optimize your growth time, temperature, and shaking speed to maximize cell density and plasmid yield as these...hours, but cultures with lower copy plasmids may need to be grown for 20 hours or more to achieve maximum...culture volume and you should ensure the shaker speed is fast enough for sufficient gas exchange in the...those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn’t work...

  13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...get this far and realize you need to apply different filters you do not need to start a new search. Simply...can narrow your search results to exactly what you need: Better searching with filters Not every plasmid...filters lets you drill down to the exact plasmid you need. The ability to apply a wide range of filters isn... your search results to match your experimental needs. Remember, only filters with results will show up...Serotype: Choose the AAV capsid or lentiviral prep needed Tags: Identify a specific marker protein Use: Similar...website and we hope it leads you to exactly what you need with every use. If it doesn’t and you find yourself...

  14. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...What is a transferable skill? Time management. I needed it when balancing a handful of demanding courses...work, bills, (at times) a social life and rest. I need it just as much in my current role as Customer Support...to do the job. You may think this means you’re guaranteed to get the job, however, many times the hiring...your boss approaches your team with a project she needs one of you to lead. At first you might think, “I...change happens at your organization, when your boss needs someone to step up, when a new venture presents ... Say your manager approaches you with glowing feedback from a client you helped. Maybe you awkwardly ...other things you have to do. Pause! Enjoy that feedback, bask in the glow of a job well done, take some...

  15. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ... thank Seeding Labs for this continued opportunity to get plasmids to the researchers who need them the...Sciences in Ghana will be receiving the third Addgene/Seeding Labs plasmid grant and plans to use the grant to...Future awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of applicants... seeding labs... seeding labs plasmid grant...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    ...review. If it’s a well-researched field, you may need to get specific to make sure your article doesn’...reviews to get a list of research papers that I needed to read. Once I had an idea of the themes in the...word of advice: when reading, don’t feel like you need to read every paper from start to finish. Find the... comment about writing: initially I felt that I needed a huge chunk of time to sit down and get anything...telling the story. Like any editing process, you will need time away from the article to be able to keep editing...normal and it means you’re on the right track!  Get feedback After you’re done editing, send it to some actual...actual experts in the field for their feedback on the scope and the content. Submit your article! Overall...

  17. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...understand the principles of gating, you’ll first need to learn a little bit of theoretical background ...software will allow you to adjust this setting as needed. To figure out the appropriate voltages to observe... through maximum current (H), the time the cell needed to pass through the laser (W) and the area under... current and thereby the signal strength. So, we need to adjust the voltage in the cytometer’s software...interest). Let’s double-click inside our gate and proceed to the next plot! Pro tip! If you only get very...gated on your cells of interest, you will likely need to exclude all duplicate events (two cells stuck...analyzed on their staining signals. For this, you’ll need to gate based on the proportion between H and A ...

  18. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ..., making it easier to produce the constructs you need. Expression vectors within this kit contain a variety...novel and powerful pyranopyridine derivatives." Proceedings of the National Academy of Sciences 113.13 (2016...terminators in this series are just the tool you need. The plasmids in this portion of the BIOFAB Plasmid...-time observation of metabolite production." Proceedings of the National Academy of Sciences 113.9 (2016...pastoris depending upon your specific protein folding needs. The pCri system has recently been used to study...adaptive evolution of antibiotic resistance." Proceedings of the National Academy of Sciences 109.52 (2012...regulation using the PUF protein family scaffold." Proceedings of the National Academy of Sciences 108.38 (2011...

  19. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...correction (about 4%) is needed to see muscle improvement, with only 30% correction needed for normal function...multiple requirements must be met. First, CRISPR would need to be delivered to both cardiac and skeletal muscle... editing occur, the CRISPR components would only need to be expressed for a short period of time, which... mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining (NHEJ) ...therapy closer to the clinic, much work is still needed. First, CRISPR delivery must be optimized to: 1...obstacles remain for CRISPR gene editing, but given the speed at which the technology is advancing, precision ...

  20. Reflections and Looking Ahead

    Type
    Blog Post
    ...redesigned our website to make it easier to find what you need and to bring it in line with current Web Content...global scientific community. As we enter 2025, your feedback continues to guide our innovation.  In response...search experience so you can find exactly what you need, faster. We’re also building a portal to easily ...landscape is evolving rapidly, and with it, the needs of researchers worldwide. Through our growing community...we're equipping every scientist with the tools they need to push the boundaries of discovery. As I look ahead... E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F., Bradbury, A. R. M., Cable...
Showing: 161 - 180 of 824 results