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We narrowed to 629 results for: EMB

Showing: 161 - 180 of 629 results

  1. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...is rare these days), and a room of 5-30 faculty members. The session lasts up to an hour. You will get ...outcome with some specifics, the search committee member cannot either. Therefore, you need to be able to...idea to bounce your ideas off of senior faculty members who can help you identify overly ambitious goals...you will be interrupted frequently by committee members. As mentioned above, plan to give your 10 minute... possible to do? Research accomplishments are remembered for the problem solved, not for the amount of...faculty positions. You need to practice with faculty members that have served on search committees. If possible...

  4. Deck the Lab 2022!

    Type
    Blog Post
    ...pic.twitter.com/rExV3b8Q8u— Michael Früh (@Michelderapfel) December 19, 2022   Congratulations to all our #DeckTheLab...Borris Aldonza (보리스) 🇰🇷🇵🇭 (@PrecursorCell) December 12, 2022          View this post on Instagram... for either contest on Twitter or Instagram by December 31, 2022.  Follow @Addgene, use the hashtag #DeckTheLab...pic.twitter.com/rDaEjxTNZX— Heng Chooi (@YitHengChooi) December 4, 2021   I spent a year hoarding used tip boxes.../X4eKUGnQMe— Alex Dainis, PhD 🧬 (@AlexDainis) December 18, 2017...

  5. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    ...It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing...decorating skills. To join the contest, simply embellish and adorn your lab space or your work from home...pic.twitter.com/OOrwo9feyJ — BTC Institute (@BTCInstitute1) December 23, 2020 Contest 2: Deck the Lab - Home Edition...pic.twitter.com/qDylF8TdHH — Matthijs Dorst (@MattNeuro) December 4, 2020 Here’s how to enter: Post your picture... for either contest on Twitter or Instagram by December 31, 2021. Let us know which contest you are entering...

  6. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...accessible to your team members only, under the specific permissions set for every member of your team. Do you...pricing, it is good to know how many of your lab members will use the ELN. If your lab is planning to grow..., notifications, sharing files, assigning team members to certain tasks, etc. How is security and user...to assign specific permissions to different lab members to avoid any issues later on. Do you need external...software solution that makes it much easier for lab members to manage and organize their work at every step...

  7. Five Popular Model Organisms

    Type
    Blog Post
    ...an ideal model organisms for embryonic development studies as their embryos are completely transparent...., and produce 50-300 eggs at a time. Zebrafish embryos are also laid and fertilized externally, allowing... them. Scientists can simply inject one-celled embryos with DNA or RNA to edit their genomes or to create...be easily visualized in a living organism from embryonic development to adulthood. This ability to genetically... and integration of transforming sequences." The EMBO journal 10.12 (1991): 3959-3970. PubMed PMID: 1935914...

  8. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... Cell Free Protein Expression Kit FREQ Seq PUF Assembly Kit Below you’ll find information about what ...engages the ribosome for translational complex assembly. PhD student Dejan Gagoski used SITS to create...Academy of Sciences 109.52 (2012): 21408-21413. PUF Assembly Kit PUF proteins are composed of repeat segments...The Zhao lab has made this easy with their PUF Assembly Kit. The kit contains all the variant PUF segments...Sequence‐Specific Detection of RNAs in Two Colors."ChemBioChem 17.10 (2016): 895-899. Choudhury, Rajarshi,...15875. See Kendall Morgan’s blog post on the PUF Assembly Kit for some insights on its potential from its...

  10. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...fibrosis transmembrane conductance regulators (CFTR) (Kamada et al., 2023). CFTR is a membrane protein ... epidermal growth factor receptor (EGFR), a transmembrane receptor tyrosine kinase essential for cell ...real time treatment with EGF. Due to the lack of membrane permeability of the LgBiT protein, it only binds....15426 Promega Corporation (2025). Illuminating Transmembrane Proteins Using HiBiT CRISPR Cell Lines. Promega...www.promega.com/resources/pubhub/2025/illuminating-transmembrane-proteins-using-hibit-crispr-cell-lines/ Shibata...

  11. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    .... Get the PUF Assembly Kit! Tips and tricks For those interested to try the PUF Assembly Kit out for themselves...tool…designer PUF proteins! A newly available PUF Assembly Kit makes it possible to devise RNA binding proteins...factors (PUF). In a single step, researchers can now assemble designer PUF domains for RNA specificity engineering... Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins for specific post-transcriptional...

  12. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...flow of electric charges across cell membranes and maintain membrane potential in response to visible light...across the axon's membrane: once a certain number of positive ions crosses the cell membrane, a threshold ...rhodopsins enable ions to flow across the axon’s membrane, thereby controlling neural activity (Bernstein...Stoeckenius. "Rhodopsin-like protein from the purple membrane of Halobacterium halobium." Nature new biology...

  13. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...also encodes the Assembly-Activating Protein (AAP) and the recently discovered Membrane Associated Accessory... AAV genome and docks with the genome onto an assembled capsid. Then Rep52/40, which also bind the capsid...generate the three VP proteins. The three VP proteins assemble into capsid at a 1:1:10 ratio (VP1:VP2:VP3). There...reading frame (ORF). While AAP promotes virion assembly, the role of MAAP has not been clearly defined...

  14. Viral Vector Guides and Plasmids

    Type
    Collection
    ...the necessary components of the viral genome to assemble the viral capsids (packaging and envelope plasmids...

  15. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence...animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a...rectify this the Shu lab first improved the self-assembling capability of sfCherry approximately 20 fold ...FlipGFP to study apoptosis in HeLa cells, zebrafish embryos, and Drosophila. They treated HeLa cells expressing...the spatiotemporal dynamics of apoptosis during embryo development. GFP fluorescence increased over time...

  16. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...three different membrane-targeting flavors – PDGFR, GPI, and SGZ, to improve neuronal membrane trafficking...segment (PDGFR TMD) that anchors the sensor to the membrane and displays it on the extracellular side. In .... PDGFR is a C-terminal fusion to the PDGFR transmembrane domain in the mammalian expression pMinDisplay...glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane domain, followed by a modified form of the cytosolic...

  17. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...they hear in their classes,” she said, “but they remember the moments when they discover something new for...focused on an emerging scientific field, BioBuilder embraces new opportunities for scientific exploration, ... Lab is a fully equipped, state-of-the-art lab embedded in LabCentral’s community of innovators that opened... Blog Synthetic Biology and the Importance of Assembly Standards Synthetic Photobiology: Optogentics ...Posts Resources on Addgene.org Guide to SynBio Assembly Standards Find Plasmids for Creating and Controlling...

  18. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...Receptor: GPCRs are also known as seven-(pass)-transmembrane domain receptors, due to the seven alpha helices...helices that transverse the cellular membrane. The receptor recognizes the appropriate ligand to activate...proteins on the intracellular side of the cell membrane. Heterotrimeric G proteins are important molecular...kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled a collection of plasmids to elucidate the role...DREADDs) (Roth, 2016). DREADDs were engineered from members of the human muscarinic receptor family and are...

  19. AAV Molecular Tools

    Type
    Collection
    ...Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...cell divisions of the C. elegans embryo when all essential embryonic genes are individually depleted via...reminded of the extent that C. elegans researchers embrace open science and share resources and tools. That...flybase. microPublication is run and maintained by members of the worm community but there are Drosophila ... lab. SapTrap is a reagent toolkit and plasmid assembly line for the creation of CRISPR targeting vectors...
Showing: 161 - 180 of 629 results