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We narrowed to 224 results for: ENDO 2

Showing: 161 - 180 of 224 results

  1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin...mNG2(11) Improved split fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017...tools for faster, easier fluorescent labeling of endogenous proteins in Caenorhabditis elegans. Goudeau et...1-10) Improved split fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017...fluorescent proteins for the visualization of endogenous proteins and synapses. Feng S et al. Commun Biol...et al. Improved split fluorescent proteins for endogenous protein labeling. Nat Commun 8, 370 (2017). https...

  2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct...composed of restriction endonucleases and methyltransferases. Restriction endonucleases cleave DNA at or near...from cleaving the DNA. A role for restriction endonucleases in plasmid addition was first described by studies...stable than the other. Here, the restriction endonuclease is more stable than the methyltransferase. When...unmethylated sites in the chromosome. The more stable endonuclease can then cut the chromosome leading to cell ...

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...posts: Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse...is less predictable. In our best case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA...about 125 nt that specifies the target, the Cas9 endonuclease that creates the DNA double-strand break (DSB...

  4. Finding Your Science Policy Path

    Type
    Blog Post
    ...teaching and mentoring others (see more in Figure 2). Starting early demonstrates not only your interest...application process so you can learn more! Figure 2: The many ways to prepare for a science policy career...regulatory approval for a new product or a well-endowed philanthropy who can shift the funding landscape...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...components, see the AAV Plasmid Elements table . Figure 2: Plasmids required for rAAV vector production. Created... pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism...the central nervous system . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024...the production of AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384...retrograde access to projection neurons . Neuron, 92 (2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021... with BioRender.com. AAVs are part of the Dependoparvovirus genus, included within the Parvoviridae family...

  6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery...purchase the Cas9 protein and a gRNA from a commercial vendor, this can often be expensive. An alternative approach...from ssDNA, which can be generated by commercial vendors such as IDT. These two components are then incubated.... "Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing." Journal...

  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic...your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin...

  8. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor In 2018, Lin Tian’s ...to gather the following types of data: Report endogenous DA release from a single dopaminergic fiber in...

  9. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...the world’s population, we account for an estimated 2% of global plastic generation. Less than 9% of plastics...recycling practices below. Reuse lab cooler boxes Some vendors take back their expanded polystyrene (a.k.a. Styrofoam...

  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...toward endothelial cells in rodent CNS AAV-PHP.V1 shows higher transduction in CNS endothelial cells (...exception is the X1 capsid for targeting brain endothelial cells, which is available in both AAV9 and AAV1...capsids derived from it, cross the BBB through an endothelial cell protein called LY6A (Hordeaux et al., 2019...functionally expressed on the surface of brain endothelial cells in C57BL/6J mice, but not in BALB/cJ mice...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally...that tests enhancer sequences outside of their endogenous chromatin environment and relies on the fact ...because it was difficult to fuse the AID tag to endogenous proteins. As described in their recent Cell Reports...genes of interest to generate mAID fusions to endogenous genes. Cell lines with the appropriate fusions...

  12. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating... using AAV to introduce epitope tags into the endogenous alleles of the p53 and PTEN tumor suppressor ...naturally occurring cancer-causing mutations into the endogenous allele of the STAG2 tumor suppressor in human...gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant...dominant mutations of a gene into the endogenous allele in cultured cells. AAV genome editing combined with ... 3. Kim, Jung-Sik, et al. "Epitope tagging of endogenous genes in diverse human cell lines." Nucleic acids...

  13. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...communicate the nuances of protein structure in a flat 2-D images. In the early days of structural biology,...the complex is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily... DNA strand, and then activate two different endonuclease active sites. Fortunately, there is never just...

  14. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...six different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini...Bioinformatics 33.7 (2016): 1099-1100. PubMed PMID: 28414855. 2. Doench, John G., et al. "Optimized sgRNA design to...Additional libraries are also available from commercial vendors. These libraries usually contain 4-12 gRNAs/gene...

  15. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused...optigenetically and chemically excited a class of enteroendocrine cells within the intestines known as neuropods...

  16. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag has been ...separation of detection of a mutant protein vs. endogenous WT levels of the same protein when both are expressed...

  17. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...The choice is non-trivial, however, as FPs are tremendously complicated entities with a large range of characteristics...

  18. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, ...signaling can also modify other properties such as endogenous gene expression, which can lead to changes in...with the goal of creating receptors with less endogenous activation and reduced off target activation ...

  19. Of Myc and Men

    Type
    Blog Post
    ...promising monoclonal antibody with high specificity. (2) Narrow down the specific epitope that your antibody...impossible if you wanted to distinguish between endogenous and exogenous versions of the same target. In.... In two studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually...

  20. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of...various signals. Over-production of vascular endothelial growth factors (VEGF) in tumors is attributed...
Showing: 161 - 180 of 224 results