We narrowed to 258 results for: GFP

1. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   ... enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One caveat...

2. SARS-CoV-2 Pseudotyped Virus

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   ...David Nemazee 161030 Zhou-pLOV-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter...resistance. Christopher Vakoc 138152 pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector ...vector expressing destabilized EGFP. Neville Sanjana 164432 pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter...

3. New England Biolabs Cell-Imaging Plasmid Collection

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   ...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag...

4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple...

5. Hot Plasmids February 2024

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   ..., Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral...

6. Cloning Mammalian Cells with the Agarose Method

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   ...select both over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio...

7. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular...

8. CRISPR 101: RNA Editing with Cas13

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   ...which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial ...

9. Antibodies 101: Immunoprecipitation

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   ...In cases such as this, an epitope such as c-Myc, GFP or V5 can be used to tag the protein of interest ...

10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed mutations,...

11. Tips for arabidopsis transformation

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    Blog Post

    ...terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There...

12. Simplify Cloning with in vivo Assembly

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    ...mutation can be introduced in a gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications...

13. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage...

14. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...Fluorescent Protein Aggregation Catch Up On Your GFP Basics Get Advice on Choosing Your Fluorescent Protein...

15. The Challenges of Cell Culture

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    ...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...

16. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted...

17. Tips for Screening with Yeast Two Hybrid Systems

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    ...in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with a transcriptional...

18. Tips for Using FRET in Your Experiments

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    ...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-...

19. Plasmids 101: The Promoter Region – Let's Go!

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    ...Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your...

20. Chemogenetics AAV Preps

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    ... 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent 1 Scott Sternson 228902...119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP Cre-dependent 5, 9 Scott Sternson... 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson 119744 AAV...AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson 121538 pAAV SYN1... Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent Cre...