We narrowed to 259 results for: GFP

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre....

2. Zhang Lab CRISPR Page

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   ...activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA...(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion ...PX552; U6 promoter-driven; for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: ... recombinase-2A-EGFP-KASH 60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone...recombinase-2A-EGFP-KASH and an sgRNA. #60231 - AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A ...SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro...

3. Trimmer Lab NeuroMab Collection

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   ...199419 Anti-GFP [N86/38R-2b] GFP Aequorea victoria Mouse IgG2b 199420 Anti-GFP [N86/8R-2b] GFP Aequorea ...206719 Anti-GFP [N86/38R-1] GFP Aequorea victoria Mouse IgG2a 206720 Anti-GFP [N86/8R-1] GFP Aequorea victoria...Rat Mouse 206743 GFP scFv [N86/44] N86/44 scFv GFP Aequorea victoria Mouse 206744 GFP scFv [N86/20] N86...short and long Rat Mouse IgG2a 114492 Anti-GFP [N86/38.1R] GFP Aequorea victoria Mouse IgG2a 114493 Anti-NGL...glutamate receptor Rat Mouse IgG2a 177572 Anti-GFP [N86/8R] GFP Aequorea victoria Mouse IgG2a 177573 Anti-...N479/107R] VAPA/B Mouse IgG2a 188164 Anti-GFP [N86/44R] GFP Aequorea victoria Mouse IgG2a 188165 Anti-.../22R] Prrt2 Mouse Mouse IgG2a 188166 Anti-GFP [N86/20R] GFP Aequorea victoria Mouse IgG2a 188167 Anti-...

4. SARS-CoV-2 Pseudotyped Virus

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   ...David Nemazee 161030 Zhou-pLOV-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter...resistance. Christopher Vakoc 138152 pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector ...vector expressing destabilized EGFP. Neville Sanjana 164432 pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter...

5. Cloning Mammalian Cells with the Agarose Method

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   ...select both over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio...

6. Hot Plasmids February 2024

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   ..., Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral...

7. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple...

8. New England Biolabs Cell-Imaging Plasmid Collection

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   ...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag...

9. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   ...used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed mutations,...

10. Tips for arabidopsis transformation

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    Blog Post

    ...terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There...

11. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...mutation can be introduced in a gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications...

12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage...

13. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...Fluorescent Protein Aggregation Catch Up On Your GFP Basics Get Advice on Choosing Your Fluorescent Protein...

14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular...

15. CRISPR 101: RNA Editing with Cas13

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    ...which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial ...

16. Antibodies 101: Immunoprecipitation

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    ...In cases such as this, an epitope such as c-Myc, GFP or V5 can be used to tag the protein of interest ...

17. The Challenges of Cell Culture

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    ...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...

18. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted...

19. Tips for Screening with Yeast Two Hybrid Systems

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    ...in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with a transcriptional...

20. Tips for Using FRET in Your Experiments

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    ...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-...