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We narrowed to 243 results for: GRN;

Showing: 161 - 180 of 243 results

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Plasmids 101: Screens vs. Selections

Type

Blog Post

..., genome-wide knockout screens are possible with gRNA libraries targeting all known genes. This is a perfect...
How to Keep a Lab Notebook for Bioinformatic Analyses

Type

Blog Post

...for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...
dTAG - You're it!

Type

Blog Post

...CRISPR plasmids for protein tagging Find validated gRNAs ...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

...genomic locus in any cell type. By varying which sgRNAs are used with FIRE-Cas9, experiments can be designed...
CRISPR 101: Cas9 vs. The Other Cas(s)

Type

Blog Post

...good reason. It is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it ...
Popular Retroviral Vectors and Their Uses in Scientific Research

Type

Blog Post

... the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher...
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Type

Blog Post

...variable portion of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

... system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA without cleavage until...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

...is mediated by recruitment of MS2-functionalized gRNAs. Find the yeast CRISPRa plasmids here Reduce ...
Hot Plasmids: Winter 2025

Type

Blog Post

...of PE protein components (Figure 1), an altered epegRNA delivered with an enhanced pU6cm promoter, viral...
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

...dedicated to expressing Cas9, while the other houses the gRNA and scaffold. If you don’t feel like doubling the...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

...transfect/inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA- and Cas9-expression...
CRISPR 101: RNA Editing with Cas13

Type

Blog Post

...edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine...
CRISPR Antimicrobials

Type

Blog Post

...Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Type

Blog Post

...protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene...
CRISPR-mediated Plant Base Editors

Type

Blog Post

...expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers...
Viral Vectors 101: Preparing Pooled Libraries

Type

Blog Post

...Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled libraries at Addgene...
Twenty Years of Sharing: Expanding Accessibility

Type

Blog Post

...Replication What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go...
Fluorescent Proteins 101: When GFP lets you down

Type

Blog Post

...can bind loci in the genome that are defined by a gRNA. Since Cas9 is a protein, a fusion with GFP allows...

Showing: 161 - 180 of 243 results

Results per page:

Plasmids 101: Screens vs. Selections

How to Keep a Lab Notebook for Bioinformatic Analyses

dTAG - You're it!

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

CRISPR 101: Cas9 vs. The Other Cas(s)

Popular Retroviral Vectors and Their Uses in Scientific Research

Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Hot Plasmids and Viral Preps - March 2021

Hot Plasmids and Viral Preps - January 2021

Hot Plasmids: Winter 2025

The AAVantages of AAV in CRISPR Screens

Plasmids 101: Knockout/Knock-In Plasmids

Google Forums Round Up: First Impressions of NgAgo

CRISPR 101: RNA Editing with Cas13

CRISPR Antimicrobials

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

CRISPR-mediated Plant Base Editors

Viral Vectors 101: Preparing Pooled Libraries

Twenty Years of Sharing: Expanding Accessibility

Fluorescent Proteins 101: When GFP lets you down

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