We narrowed to 444 results for: HAL;
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TypeBlog Post... (such as Nicotiana benthamiana or Arabidopsis thaliana (Xing et al., 2016)). During Agrobacterium-mediated...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...
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Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...the power 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on ...described by the t½, which is the time after which half of the initial fluorescence intensity remains. The... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...2015; Koike-Yusa et al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert... 26190106. PubMed Central PMCID: PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...Tebo et al. Nat Commun. 2019 Arnaud Gautier HaloTag TagBiFC allows exogenous labeling for single-molecule...and reversible complementation. TagBiFC (split HaloTag) provides maximum flexibility in color labeling... -
How to Prepare for an Industry Interview
TypeBlog Post...research project. In total, the interviews lasted from half to all of the day. This whole process may seem ...carefully. And even if you don’t have technical challenges, your interviewers might. You’ll probably still... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...𝛽-globin), which cause sickle cell disease and 𝛽-thalassemia, can be mitigated with the expression of fetal... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post... at the Institute of Molecular and Clinical Ophthalmology Basel recently took a different approach to ...to test for cell type-specific expression of GFP. Half (113) expressed in retinal cells four weeks post-injection... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...topic or that were published by the lab down the hall. Scientific journals: They publish papers, so naturally... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...have shown that the conditions Sotos-type 2 and Marshall Smith Syndrome are due to mutations in the NFIX...diagnosis of NFIX-related syndromes (Sotos-type 2 and Marshall-Smith) and could be expanded to NFIA-, NFIB- and... -
Hot Plasmids - October 2020
TypeBlog Post...synaptic connections and physiology is particularly challenging as they are great in number, generally very small...H2B-FT-red is long lasting and was shown to have a half life of 84 hours in primary mouse embryonic fibroblasts... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...that can impact reproduction include embryonic lethality, a short lifespan, or infertility and can arise... number or expression level effects. Another challenge that may arise when crossing mice with multiple... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post... tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are protein ...With the split system, only cells that have both halves of cGAL can drive expression of your gene of interest... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves...after transfecting cells, replace the medium with half the volume typically used (e.g., use 5 mL medium... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...processing and contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation...39322032 No terminator is 100% efficient at halting transcription of the template and initiating the... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...real-time, posing a huge challenge to studying epigenetic changes over time. This challenge was difficult to ... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post... publish or attend conferences makes it very challenging to compete in today’s tough academic or industry...team. We have confidence that we can take on any challenge and quickly become experts in something, even ... -
PhD Applications After COVID
TypeBlog Post...program itself. Picking a mentor comes with many challenges, and the flood of interview requests and visitation...a travel day to an online call in response to challenges on their end. Though the quick changes in plans... -
10 Basic tips for mammalian cell culture
TypeBlog Post...Turning on the UV light 3-4 times a week for up to half an hour can help keep your hood contamination free...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...this complex, developing Cas3 tools has been challenging. It can be difficult to ensure comparable expression..., though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have one...