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Showing: 161 - 180 of 375 results

  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...Chicken IgY domains are especially useful for multi-color staining of tissue because they have limited cross-reactivity...cultured cells and tissue sections Great for multicolor staining of tissue since it won’t cross-react...

  3. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...tried-and-true protein expression control tools as well as explore some new innovations. Whether you want to turn ...introduced gene or over the native locus? Let’s explore each of the options together. Turn it off Turn...

  5. Working with Nuclear Receptors

    Type
    Blog Post
    ...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ... Professor of Molecular and Cellular Biology at Baylor College of Medicine and Editor-In-Chief of the ...

  6. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...standard method for purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation...practical guide for using the AdEasy System. 3. Jager, Lorenz, et al. "A rapid protocol for construction and ...

  7. Hot Plasmids - October 2020

    Type
    Blog Post
    ...synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity without the...Guo and her colleagues at Yale University fused a color-changing fluorescent timer (FT) protein to the histone...

  8. CRISPR in the Clinic

    Type
    Blog Post
    ...example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential in mice with a dominant...component, CAR. Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in ...

  9. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...quickly. Of course, you might have less time to explore scientifically since you can’t afford to spend ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

  10. Of Myc and Men

    Type
    Blog Post
    ...ll dive into those questions in this post as we explore… the life and times of the myc tag.  Origins of...that their new monoclonals would help the field explore Myc’s role in cell proliferation and tumorigenesis...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    ...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...concentration of a chaotropic salt (guanidine hydrochloride for the plasmid purification protocol and guanidine...

  13. CRISPR Antimicrobials

    Type
    Blog Post
    ...but also alter an individual’s native, beneficial flora. Currently, there is no antibiotic strategy available...population, represented here by cells of different colors, specifically targets bacteria with the given genomic...

  14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...processes. Re-engineering GFP to increase its range of color and application Almost as soon as its sequence... not listed here, many permutations within each color also exist with only slight variations separating...

  15. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... their selection techniques to come up with new colors (Fig. 3), such as Radish and Carrot, and further... ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...

  16. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...other disciplines, or from other countries, to explore new connections to our research. So how can we ... 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring video abstracts in science journals: An overview...

  19. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...into the content of Addgene’s aptamer soup by exploring the development and applications of fluorescent...aptamers Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in...
Showing: 161 - 180 of 375 results