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  1. CRISPR in the Clinic

    Type
    Blog Post
    ...co-injection of Cas9 and a single-guide RNA targeting the mutant allele. When all was said and done, the researchers...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...

  2. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ... if you want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region... hand, if you are trying to knock-in a patient mutation or need to make a precise amino acid change, there...

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...additional SapTrap plasmids used for introducing point mutations that was published in a microPublication (Schwartz... E. M. "SapTrap vectors for introducing point mutations with unc- 119 + selection." MicroPublication 1...

  5. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...with a deletion-mutant rabies virus. Retrograde neuronal tracing with a deletion-mutant rabies virus. Pubmed...

  7. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...orientation limiting FRET efficiency, circular permutation (Baird et al., 1999) of the fluorescent protein...References 1. Baird, Geoffrey S., et al. "Circular permutation and receptor insertion within green fluorescent...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...indicated in the “Species Other” column. Relevant Mutations Indicate the amino acid change as well as any...functional consequences of that change. For example, “Mutated aspartate 123 to Lysine (D123K), lowers biosensor...

  11. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability and yield. If a plasmid...using older plates could increase plasmid loss or mutation. Let it incubate Increasing the incubation time...

  13. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing...

  14. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...outlined below (or emission wavelenght range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP)...breakdown of GFP, it's variants, and their relevant mutations, check out Marcy's post from last week on GFP....

  17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors two surface exposed...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...

  18. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... but won’t tell you if your products have any mutations.  There are lots of different cloning strategies...expected sized PCR product doesn’t mean there aren’t mutations in your insert. Make sure to submit multiple positive...

  19. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...the deletion reduces the risk of insertional mutagenesis by limiting the interaction between viral LTR...potential for oncogenesis through insertional mutagenesis. These risks are largely based upon the vector...

  20. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...determine the presence and quantity of DNA, RNA or a mutation of interest. Together SHERLOCK and DETECTR demonstrate...SHERLOCK could also detect low-frequency cancer mutations from cell-free DNA fragments as well as health-related...
Showing: 161 - 180 of 395 results