...control. C) Iterative transduction workflow overview. Image from Sun et al. 2021. The Organoid EasyTag ...of the receptor-based SL fluorescent biosensor. Image from Chesterfield et al., 2020     Chesterfield... calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128...

...These cells were imaged with a 10X objective and were split 2 days before these images were taken. Last... Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents...Fisher, 12605010) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment... + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride Close the bottle and mix by inverting...

...thanks to our guest blogger Magdalena Julkowska from KAUST, Saudi Arabia. Magdalena Julkowska did her Ph.D.... This post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. ...

...Once introduced, fluorescent proteins were best imaged after tissue clearing with SeeDB2 (Ke et al., 2016...tissue samples for High-resolution fluorescence imaging using SeeDB2." Bio-Protocol 8 (2018): e3046. Livet...PMC6245733. Susaki, Etsuo A., et al. "Whole-brain imaging with single-cell resolution using chemical cocktails...

...If you work in a lab that regularly does immunoimaging, there’s likely a large collection of antibodies...abstract protein during your sample prep process, imagining how it starts and if any steps could affect its...frustration when designing and troubleshooting immunoimaging assays. It can also elucidate information about...

... occurs in the P.1 and B.1.351 variants tested. Image from Garcia-Beltran et al., 2021 with permission...Neutralization was quantified in terms of luminescence. Image from Garcia-Beltran et al., 2021 with permission...convalescent sera with various pseudotyped virus. Image from Garcia-Beltran et al., 2021 with permission...

... Pamela J. Hines PhD, Senior Editor at Science Magazine. Although we only walk one path at a time, the...spotted an opening for being an editor at Science magazine. That one I just could not resist applying for... Pamela J. Hines is a Senior Editor at Science magazine, published by the American Association for the...

...demonstrated the ability to be used as an endogenous RNA imaging tool.   Fig. 1: Csm, shRNA, and Cas13 ...ultrasound (FUS) to heat the tumor area, as the image below shows:    This system ablates tumors just...on the Anti-GFP [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here...

...which makes it possible to do things like in vivo imaging.   Excitation with NIR light also produces less...photosensitizers had similar rates of off-target damage to neighboring cells. TL;DR MG-2I-dL5** is a NIR...inactivation/cell ablation while having limited collateral damage to bystander cells. Are you ready to use MG-2I-...

...Figure 1: Cas9 binding to the cleaved DNA duplex. Image from Richardson et al., 2016. We next wondered...donor DNA interaction with the Cas9-DNA duplex. Image from Richardson et al., 2016.   dCas9 enables... replacement frequencies that are an order of magnitude greater than transfection approaches. Moreover...

...correct. Final touches We try to use figures and images to help illustrate our points and break up walls...Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer upon request...appropriately licensed and cited figures from papers or image sharing repositories. Then we format, do a final...

...types of breaks in tens of minutes – an order of magnitude faster than HDR. NHEJ is consequently the principal...this pathway and does not consider the missing or damaged nucleotides that are common to biological sources...DSB (blunt or near-blunt ends without nucleotide damage) such products are rare, probably accounting for...

...Two-Photon imaging was used to generate a 3D finemap of neurons in the mouse brain. MouseLight images can be...uniform and standardized way (Oh et al., 2014). Images yielded from this study can be found on the Allen... Lutton, E.M., Mu, D., Hudry, E., Hyman, B.T., Maguire, C.A. and Ramirez S.H. (2017). Trafficking of adenoassociated...

...of Gut microbiome by In situ Conjugation (MAGIC). With MAGIC, scientists introduce an Escherichia coli...genetic tools for microbiome studies.  Find the MAGIC plasmids here Paper-based synthetic biology approach...

...single domain antibody. To create their RANbodies, Yamagata et al used previously published nanobody sequences...RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find a RANbody ...the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-nanobody fusions (RANbodies...

...If you’re reading an article with images, now you have words, images, and links to pay attention to. If...If you’re watching a video, you now have moving images, noises and music, spoken words, and any onscreen...

...Imagine being able to determine whether two proteins are within 10 nanometers of each other, or measure...FRET! Read on to find out more about this amazing imaging technique and find further tips for using FRET ...demonstrated the use of single-molecule FRET for imaging biomolecules in live cells (Sustarsic and Kapanidis...

...ICV, intracerebroventricular) to minimize tissue damage; however, the spreading of vector to the area of...needle should be as thin as possible to minimize the damage to the brain tissue. The injection speed has to...from the injection site, and also to minimize the damage of tissue due to increased pressure of the added...

...different FingR constructs and experimental results. Image from Bensussen et al., 2020. The FingR constructs... proteins with different subcellular locations. Image from Goudeau et al., 2020. Goudeau, J. bioRxiv... media without IL-3 show only red fluorescence. Image from Eastman et al., 2020. The researchers tested...

...PMID: 23654268. FLINC biosensors for live-cell imaging of biochemical activity Article contributed by ... new super-resolution techniques for live-cell imaging of the dynamic changes in biochemical activity ...energy transfer), and FLIM (fluorescence lifetime imaging microscopy) assays. ShadowG is darker than other...