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We narrowed to 550 results for: NOL

Showing: 161 - 180 of 550 results

  1. Typing CRISPR Systems

    Type
    Blog Post
    ...classification system for the array of CRISPR technologies. Learning the general principles of this classification...the first taught when introducing CRISPR as a technology. There are three subtypes, A–C, with the beloved...Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing. Cell ...Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system...

  2. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...their hot-off-the-presses papers, the awesome new technology they’re utilizing, and the tricks they’ve used...plasmids in your own lab. Summaries of the latest technologies and breakthrough research. Insights about ...a special focus on cloning and plasmid-based technologies. Commentary about scientific sharing and the...

  3. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation This video goes over... over AAV vector purification using an iodixanol column gradient. We'll show the loading and unloading...instructions. AAV titration by qPCR using SYBR green technology Here’s some advice on how to successfully set...

  4. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ... and Diplomacy, with a focus on international technology management, from the Fletcher School at Tufts...investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie Chen is a co-founder...and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software Engineer...

  5. Deep Dive: qPCR

    Type
    Blog Post
    ...; Hellemans J; Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl MW; Shipley GL; Vandesompele J; Wittwer...abundances using SYBR Green I and real-time RT-PCR. J. Immunol. Methods 283:291–306.Panina, Y., Germond, A., Masui...Reproducible Data the First Time, Trends in Biotechnology, Volume 37, Issue 7, 2019, Pages 761-774, SSN... for low copy number target DNA. Journal of Immunological Methods 270 (2002) 119 – 133Tichopad A; Dilger...

  6. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ... the United States, and many universities and Technology Transfer Offices were, at least, temporarily ...                           California Institute of Technology             Johns Hopkins University                           ...will initiate a conversation with you and your technology transfer office to discuss the possibilities ...

  8. Advice for Choosing a Research Project

    Type
    Blog Post
    ...address a topic in a mechanistic way using the technology available.” Choosing a research project “entails...entails good judgement with the current technology to identify a problem, asking what approach will I take... importance of combining “new discoveries or technologies increase chances that your project will be fresh...

  9. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids...mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes...colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University...

  10. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...many developments in science and the plasmids, technologies, and researchers behind them. As part of our...started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed to provide...will be sure to keep our eyes open for any new technologies that will bring about the next revolution in...

  11. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...tools by making their own fluorescent protein technologies widely available to the research community. ... a key advantage over other inducible enzyme technologies. She hopes that other researchers will be able... HMOs. Current HPLC-MS and fluorophore-based technologies are too time consuming in the first case and...

  12. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... to Predict Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389...adverse reactions to drugs: adverse reaction terminology. WHO Collaborating Centre for International Drug....2022.952326Joubert, M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, M. P., Herskovitz...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... via Single-Cell RNA Sequencing. Frontiers in immunology, 12, 730825. https://doi.org/10.3389/fimmu.2021.730825... Gradinaru V, Fox AS. (2023). Advances in AAV technology for delivering genetically encoded cargo to the...throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419...widespread gene transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt...tropism of viral vectors in tissues. Nature Biotechnology. https://doi.org/10.1038/s41587-022-01648-w ...

  15. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...acids in the domain). Yan et al. from Arbor Biotechnologies found that RspCas13d accessory protein RspWYL1...functional in multiple species (read Arbor Biotechnologies' press release about their Cas13d deposit here...Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources on the Addgene Blog:...

  16. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...CRISPR 101" post. Next-generation sequencing technologies are the gold standard but they remain cost-prohibitive...introduce digital PCR as an emerging validation technology. Digital PCR has several advantages over mismatch...CRISPR-Cas nucleases in human cells. Nature Biotechnology 31, 822–826 (2013). PubMed PMID: 23792628. PubMed...

  18. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...γ-retrovirus packaging cell line developed by Garry Nolan at Stanford (Swift, 2001) contains Gag-Pol and either...resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient...cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: 10.1002/...

  19. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...to essentially any DNA sequence desired. This technology allows you to create a pair of designer proteins...on traditional homologous recombination. That technology had been used for decades to generate knock-out...That’s the revolutionary aspect: TALENs take a technology that was difficult for all but the most experienced...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others. Each method is unique...biologists have harnessed it to evolve cloning technology. CcdB: A potent toxin... The ccdB gene, located...thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially a ...
Showing: 161 - 180 of 550 results