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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 423 results for: NUC

Showing: 161 - 180 of 423 results

  1. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

  2. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive staining...completely removed.  Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately...bacterial or mammalian expression, targeted to nucleus, cytoplasm, or mitochondria A genetically encoded...Photosynthesis-dependent H 2 O 2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nat...cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7. pii: 32638. Kai Johnsson...GRAB-HTR6 A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Cell... protein pH biosensor to detect concentrative nucleoside transport. J Biol Chem. 2009 Jul 31;284(31):20499...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...associated reverse complement including additional nucleotides for cloning and expression purposes. NOTE: Here... BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after the CACC sequence and before the 20-mer...pX330 vector is enhanced by the inclusion of a G nucleotide after the CACC sequence. Add a C at the 3’ end...Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated...

  7. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for...design tools and ordered as standard desalted oligonucleotides with the appropriate overhangs: The plasmids...quick ligation of annealed and phosphorylated oligonucleotides containing the guide target sequence: Up to...

  8. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though ...restriction sites into which you can clone your 20 nucleotide guide sequence of choice (for generation of a...

  11. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...Soup by Aliyah Weinstein RNA aptamers, which are nucleic acid sequences that fluoresce upon binding a small...Transfection by Alyssa Cecchetelli The introduction of nucleic acids into bacteria or eukaryotic cells is a common...

  12. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the...

  13. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...addition of 4-hydroxytamoxifen, it can move to the nucleus for activity. They also developed one of these ...gene activation and editing. Read the paper in Nucleic Acids Research Find the drug inducible Cas9 plasmids...

  14. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to living cells...pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid...

  15. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...and binds the inverted repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released...transposon can now bind a strand of DNA with a TA dinucleotide (there are many such sites in the human genome...

  17. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...

  18. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...tertiary structure when transcribed which aids in nuclear export of mRNA and helps increase the expression...The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes...
Showing: 161 - 180 of 423 results