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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 768 results for: Ncl

Showing: 161 - 180 of 768 results

  1. Fostering communication in remote collaborations

    Type
    Blog Post
    ...stays on the same page. Ideally, these meetings include all collaborators, from the lab members who are...like Zoom or Google Hangouts is a great way to include researchers from different locations. Having virtual...to your collaborators. This helps everyone feel included in the project and accelerates the pace at which...and morale. Especially when your collaboration includes scientists working in different time zones, it...

  2. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ... and copy number of the plasmid) and typically include a gene that is advantageous for survival, such ...range of applications. Some common plasmid types include cloning plasmids, expression plasmids, gene knock-down...the many things that plasmids can be used to do include: Produce large amounts of a protein so that scientists...the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, how to choose...

  3. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ... Now you’re wondering if your favorite gene is included in other CRISPR screens and whether that data ... each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system, sgRNA...-depth walk through of how to use ORCS:     Include your CRISPR screen data in ORCS  Researchers who...who wish to have their CRISPR screen data included in the BioGRID ORCS CRISPR database are encouraged ...

  4. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...submissions via email: [email protected] Entries include accession number, species, and assigned category...profiling for human, mouse, and dog cell lines is included when available, and can be submitted via email...Depending on available information, entries may also include whether a cell line is known to be genetically ... cited. If the information seems incomplete or unclear, Bairoch follow up via email to the authors of ...

  5. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ... browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...have expanded our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several... for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. ...

  6. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...distributing lentivirus (with many CRISPR tools included among the preps that are currently available) ... AAV preparations have worked well in my lab, including in applications where we have delivered them in...The blog provides tips for working with viruses including advice on how to titer lentiviral preps, troubleshoot...on each viral prep’s specific material page and includes western blots as well as other expression data...

  7. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...the ins and outs of this classification system, including its history, biology, and usages in the lab. History...peptidoglycan membrane. Common gram positive bacteria include staphylococci and streptococci. Gram negative bacteria... lipid membrane. Common gram negative bacteria include salmonella and E. coli. Because of their thicker... For more specific procedural instructions, including timing, preparing the smear, and mixing the stains...

  8. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...span four continents and research diverse topics including microbiology, brain injury, drug discovery, and...PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic engineering...studied various aspects extreme life on earth including extremophile microorganisms, biomarkers, and recently...fluorescent signals from the sensor, and send a signal including its position in the river back to the user.   ...

  9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...ways foreign DNA can be introduced into cells including transformation, transduction, conjugation, and...often comes from adjacent lysed bacteria and can include plasmid DNA or fragmented DNA released into the...Addgene. For more information on viral vectors, including transduction download our Viral Vectors 101 eBook...into a eukaryotic cell through non-viral methods including both chemical and physical methods in the lab....

  10. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...GCE, each component of the experiment system (including media, cell type, and growth temperature) may ...to use a protocol that you’ll have to rework to include reagents accessible to your lab. The solution GCE...is for recombinant expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses... While they’ve shortlisted several protocols, including their phosphoserine ncAA protocol, to optimize...

  11. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...example, Addgene’s contributions by Tyler Ford included reagent sharing and were presented at the ASPB...at this specific conference, and I was able to include and exclude different modules for this workshop...learned: Since early 2018 the team has grown to include experts who could help provide new materials and...assembled the right people at the right time. Be inclusive and seek solutions by working with communities...

  12. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...also be designed with other DNA-binding domains, including the commonly used LexA and rTetR systems such ...could be delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord....easily adaptable to neuroscience applications, including optogenetics, and they should make it much easier...Other proteins may also be utliized as scaffolds, including the set of red fluorescent proteins. The T-DOGGs...

  13. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...contains both fragments of split mVenus (aa 155), includes I152L in N-terminal fragment An enhanced recombinant... Dan Mulvihill mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment Constant rate of ...  When designing experiments, it’s important to include proper controls to assess the behavior of the split...Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools...

  14. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    .... This tool-kit includes an array of FPs as well as additional useful tags, including 3xFLAG epitopes,... chromatin. A fluorescent protein can still be included for visualization purposes, but the key component...green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte, and...

  15. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... delivery technologies have been investigated including lentiviruses, adenovirus, and inorganic delivery...additional safety benefits: they lack all viral genes, including those that are responsible for integration into...capable of infecting a wide range of cell types including but not limited to muscle, liver, and brain cells...clinical trials have been directed at diseases including but not limited to hemophilia B, LPL deficiency...

  16. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...for affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman...implementation and compliance for the FLSA changes, including finding money in already tight lab budgets and...their advocacy skills. Friday’s symposium will include a discussion on effective advocacy strategies on... on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington ...

  17. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...unstained flow controls). Spectral flow cytometers include a tool called “autofluorescence extraction” which...various tissue types varies, it is critical to include an unstained sample for each tissue type you are... the expression levels of the markers you are including in your panel. Highly expressed markers should...during unmixing. Additionally, it is critical to include unstained beads or cells within your reference ...

  18. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...Over the years, Addgene’s repository has grown to include many CRISPR tools.  Distributing and depositing...this tell us? Well, it’s hard to draw any exact conclusions, but it certainly suggests that Addgene depositors...approximately 104 plasmids shipped per working day, including appropriate MTAs and customs forms. Of course,...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story that...

  19. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...are required for any application. If you want to include a homology directed repair (HDR) template to create... table below summarizes the various components included with each expression system as well as suitable...components into mammalian cell lines is quite broad and includes several different methods, so we've summarized...Difficulty Hard Hard Hard These tables are not inclusive of all methods, nor are these methods limited ...

  20. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...addressed in a somewhat cursory manner, their inclusion here adds much needed context to understand every...to see the dirty laundry exposed here, but it’s unclear how much they will actually care. The section of...state of science would be complete without the inclusion of some interesting case studies, and Zimmer provides...who originally isolated the GFP gene but was not included amongst the Nobel prize winners for developing...
Showing: 161 - 180 of 768 results