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We narrowed to 349 results for: Plat

Showing: 161 - 180 of 349 results

  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...may lead to loss of the plasmid, and using older plates could increase plasmid loss or mutation. Let it...smaller cultures, especially those in deep-well plates, require a faster shaking speed for proper aeration...

  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...publication. Addgene makes this easy by providing a template citation for you to use in your Materials and ... Use the Addgene catalog number to easily find template citations for Addgene plasmids you have used in...

  5. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...Addgene launched our AAV Data Hub as a community platform for the sharing of that exact data. Here, requestors...develop new systems, new partnerships, and new platforms, from our early engagement with UPenn to our launch...

  7. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...amplification. Transformed E. coli are allowed to grow on plates or in liquid culture to amplify the library, followed...Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale...

  8. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...is a factor in AAV titration since the starting template is the purified viral vector solution (which contains...be mitigated by using purified viral DNA as the template instead of the intact AAV particle. Finally, ...

  9. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum discussions...to see the questions first makes Reddit a great platform for those who aren’t so great at coming up with...

  10. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...concentrations of lactate. The microfluidic screening platform developed here is also a promising approach for...other targets. Figure 6: A) Schematic of BeadScan platform for screening candidate biosensor variants for...

  11. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...Validating knock-in mutations You’ve introduced a donor template along with your gRNA and Cas9 combo and are hoping...Jonkers, Bas van Steensel. Easy quantification of template-directed CRISPR-Cas9 editing. (2018) Nucleic Acids...

  13. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...associated with an elevated mutation burden. The CRISPR platform must be optimized to reduce off-targets and patient...Hopefully you can understand that depending on your platform, off-target effects should impact your experimental...

  14. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...photometry. Plus, both designs offer promising templates for future improvements. Find PinkyCaMP plasmids...and equipment like incubators, micropipettes, and plate readers. Instead, students can use inoculation loops...

  15. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized ...Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein interactions...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... present. The reaction takes place in a 96-well plate, where primary and secondary antibodies are used...Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies Hybridoma...

  17. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...the cell to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of...repairs the DNA using the U-containing strand as a template, copying the base edit. Figure 2: Cytidine...

  18. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...whenever a new video is posted! If TikTok isn't your platform, all of our videos will also be shared on our ...

  20. We’re Going Viral This Week!

    Type
    Blog Post
    ...week, we launched our new AAV Data Hub, an open platform for scientists using AAV to find and contribute...
Showing: 161 - 180 of 349 results