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We narrowed to 601 results for: Ret;

Showing: 161 - 180 of 601 results

  1. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...copper cathode/iblot gel matrix, and filter paper. Pretty much like an actual sandwich! S'mores image from....2015.04.012 Burnette WN (1981) “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide....76.7.3116 Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels...

  2. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic...

  3. Finding Your Perfect Job After University

    Type
    Blog Post
    ...experience. Working in industry: Microbiology On returning from Ghana at the beginning of 2010, I explored...looking for other career options after 6 months. Returning to university to study a Masters of Science I ...programming. Working for a scientific nonprofit On returning to the UK, I checked out the job postings from...

  4. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    .... Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9 MOI considering that...I didn’t do this, but to do it you would need a pretty good gel image. If you are analyzing short DNA ...cancer cell line in about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research...

  5. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... was not present (Kuhlman, SK. 2008). While theoretically this strategy should work, these vectors suffered...data and can instill greater confidence when interpreting the outcome.    References and resources References...Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for...

  6. Plasmids for Stem Cell Research

    Type
    Collection
    ...MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of...MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral expression..., fully-differentiated adult somatic cells are returned to a pluripotent state using a cocktail of factors...11(3):373-86. Jaenisch Fibroblasts Hepatocytes Retroviral Mouse Direct conversion of mouse fibroblasts ...nature10323. Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse and human fibroblasts...2527-32. Wernig Fibroblasts Neural Stem Cells Retroviral Mouse Direct reprogramming of fibroblasts into...Cell Stem Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron ...

  7. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...floxed-shRNA constructs, Cre can excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox...Flp-dependent expression systems and may affect the interpretation of your experimental results. Attenuated iCre...of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description...of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description...expressing Dre and other site-specific recombinases. Return to top Other Site-Specific Recombinase Plasmids...Description Gene/Insert Vector Type Promoter PI Return to top Recombinase-Dependent Empty Backbones Find...find other Cre-, Flp-, or Dre-dependent plasmids. Return to top Recombinase-Dependent Reporter Plasmids ...

  8. Typing CRISPR Systems

    Type
    Blog Post
    ...gku241 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...Pinilla-Redondo, R., Mayo-Muñoz, D., Russel, J., Garrett, R. A., Randau, L., Sørensen, S. J., & Shah, S....

  9. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ... Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...

  10. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...Off-target activities of the RNAi Correctly interpreting your data therefore necessitates careful design..., this can make the results more difficult to interpret, and a knockout/ or knockdown approach should ...validation is critical to ensuring accurate interpretation of your results. Therefore, taking advantage...

  11. Viral Vector Guides and Plasmids

    Type
    Collection
    ... capacity. Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred... referred to as just retroviral vectors, have a relatively high transduction efficiency and only infect...genome, enabling long-term transgene expression. Retroviral vectors are popular for making stable cell lines...80–130 nm Dividing/Non-dividing Yes Low Gamma-retroviral Stable RNA < 8 kb 80–130 nm Dividing Yes Moderate...

  12. Open Enzyme Collection

    Type
    Collection
    ...Tth DNA Ligase 165574 pOpen-HSlig3 DNA Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165573 pOpen-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid...165567 pOpen-XbaIR XbaI 165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid...Thermus thermophilus (Tth) Reverse Transcriptase Return to top RNA Polymerases Addgene ID Plasmid Gene/...Polymerase 165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert...165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top...

  13. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to ...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

  14. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...developing mouse retina. To aide in their studies of the regulatory networks involved in retinal development...plasmids to study the regulatory networks involved in retinal development. Depositor...Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or ...Simultaneous deletion of Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification...

  15. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to ...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

  16. Recombinases AAV Preps

    Type
    Collection
    ...* Karl Deisseroth *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...Esteban Engel , Alexander Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Connie Cepko 69571 pAAV-EF1a-C-CreintG...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

  18. Antibody Plasmid Collection

    Type
    Collection
    ...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...libraries. Immunol Lett. 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic ...-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids...

  19. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to ...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...for pegRNA, Esp31 for PBS-RT No BFP Ervin Welker Return to top CRISPR Resources Addgene has a large selection...

  20. Chemogenetics AAV Preps

    Type
    Collection
    ...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova... dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t ...viruses would be helpful to the scientific community. Return to top...
Showing: 161 - 180 of 601 results