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We narrowed to 399 results for: SEF

Showing: 161 - 180 of 399 results

  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... the three toxins so that at least one might be useful in a broad array of pathogenic Proteobacteria. ...: AEM-00990. PubMed PMID: 31201277. Liljeruhm, Josefine, et al. "Engineering a palette of eukaryotic chromoproteins...

  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...from ATP to urotensin. The MTRIA sensors will be useful for investigating neuronal signaling and for engineering...pages to find more. We hope these tools will be useful in your future research, and we’re looking forward...

  3. A Control for All Seasons

    Type
    Blog Post
    ...and thoroughly controlled experiments.  Broadly useful controls Positive and negative controls Most biological...experiments. We hope that this blog post will be a useful starting point for you when designing rigorous ...

  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...membrane and initiate an action potential - a very useful tool for neuroscientists! Since then, scientists...Gong et al., 2020). Light-gated ion channels are useful when you want to activate or inhibit specific neurons...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...expression in a large number of cell types and are useful if you're going to be working in several different...like doxycycline (Favre et al., 2002). They are useful when you need to control the timing of expression...

  6. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...Accelerate research and discovery by improving access to useful research materials and information. Many of our...responds to changes in intracellular calcium and is useful for direct imaging of events in cells and brain...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...-targeting field. Mice have long been extremely useful in the lab – they are relatively small and easy...generating mouse models Although CRISPR is incredibly useful for generating mutations by NHEJ and generating...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...the Liu lab’s OXfCRISPR page provides many other useful resources for fly labs who wish to harness this... complex genetic elements. To demonstrate the usefulness of GAMP, the Jacks Lab constructed several GMAP-compatible...enough variation in a native genomic context to find useful mutants. CRISPR-X, a new system from Michael Bassik...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ... opted for AAV. While these benefits make AAVs useful for a wide variety of experiments, care still needs...improvements are being made to make them even more useful for therapeutic purposes. The University of Pennsylvania...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ..., lentiviral vector constructs have proven very useful due to their ability to infect both dividing and...instance. I hope you will find this information useful for your experiments. Don’t hesitate to check our...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Targeting different regions of the gene is also useful in case the gene has a weird mutation that you ...digested product may be limited and faint, so it’s useful to have as many controls as possible to confirm...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...bit of chemistry magic to produce a wide range of useful molecules linked to that substrate. If the substrate...green structure represents a fluorophore or other useful ligand, gray structure is the protein of interest...

  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...of genes provided by the user. This approach is useful for several applications as will be discussed below...different ligands. Such knowledge could be very useful for drug discovery, to dissect a complex signaling...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... and kits. Keep in mind that this technology is useful for a variety of purposes beyond what we discuss...cell-free systems. The approach is particularly useful for producing drug precursors, biofuels, and natural...

  15. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...antibody, and how is a component of the immune system useful as a research reagent? Let’s find out! What is ...  The ability of antibodies to bind proteins is useful for research applications as well because they ...

  17. Deep Dive: qPCR

    Type
    Blog Post
    ...forward or the reverse primer.  There is a list of useful primer design resources in the References and Resource...spreadsheets available for easy analysis. These useful tools don’t excuse you from understanding how the...

  18. Technical Design of a Western Blot

    Type
    Blog Post
    ...get a single-percentage gel, which is cheap and useful when you’re looking at proteins all roughly the...your choice of detection methods. They are very useful for troubleshooting or optimizing western blots...

  19. New Antibody Animation!

    Type
    Blog Post
    ...'s YouTube channel for more helpful animations, useful protocol videos, and quick tips for lab success...

  20. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...fresh new look.  We think this is a helpful and useful resource — but you don't have to take our word ...
Showing: 161 - 180 of 399 results