Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 927 results for: TIM;

Showing: 161 - 180 of 927 results

  1. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...achieve your intended next career stage and create a timeline for achieving these goals. Planning is often more...months depending on the revisions and the amount of time spent shopping a manuscript. Manuscript writing ...available the potential mentor is. If the person makes time for you, as needed, this is a strong indicator that...relatively simple experiment. However, by taking this time, you can help ensure that you understand the methodology...and dirty" experiments often eat far more of your time as they can result in spurious yet intriguing findings...productive results and thereby help decrease the time to your first manuscripts. 4. Balance focus and ...seemingly unrelated problem. However, you have finite time and resources. You typically have 4-5 years to make...

  2. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...optogenetics differ are: Timing Targeted manipulation Controlling stimulation Invasiveness  Choosing ...Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to receptors...activation (chemogenetics) or light (optogenetics).   Timing Optogenetics offers a high degree of temporal resolution...Chemogenetics does not offer the same level of control over timing. Because chemogenetics relies on chemical activation... elicit an immediate response or track the exact time of activation. Receptor activity is also drawn out...injected, this is not as precise and the offset of stimulation is gradual. Invasiveness Both optogenetic and...experimental design.   Optogenetics Chemogenetics Timing Fast activation/deactivation Prolonged activation...

  3. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...animal has been exposed to morphine or other stimulating drugs, it develops an enhanced propensity for...Mice with higher IL-1 beta production spent more time in the open arm and were less risk averse. Similar... and a dark (protected) area. When measuring the time spent in a particular part of the apparatus, it ...that the IL-1 beta overexpressing mice spent more time in the risky bright place, driven by their motivation...reflects a state of behavioral despair, and the more time the animal spends in the passive mode, the stronger...forced swim test as seen by increasing the ratio of time spent actively swimming versus passive floating....receptor protein that activates neurons upon light stimulation. When they activated the Drd1 neurons 24 hours...

  4. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ... to full use! Common conditions to optimize include incubation time, antibiotic conditions, and amount...titering and optimizing before you freeze down your viral preps!)  Once you’ve optimized your transduction...amplification protocols. Rather, each protocol is optimized for a single, specific library.  If you have a...their methods sections to see what worked.  Ready, optimize, transduce!  If you’re ordering a pre-made library... plug-and-play: you’ll still need to titer and optimize before you can transduce your cells.  Titering... scientists to perform functional titering and optimize transduction efficiency. Transduction efficiency...vary across cell types and generally requires optimization of many variables, such as seeding density and...

  5. Antibodies 101: ChIP

    Type
    Blog Post
    ...need to be optimized for each specific sample type. And, if that weren’t bad enough, optimization of one ... digestion, but is harsher and requires more optimization. Enzymatic digestion, while gentler on the sample... ChIP. If you’re using an antibody for the first time, look for one that has been validated in multiple...used, but from the difficulty of developing an optimized protocol - it’s a bit like Goldilocks and the ...one step can affect the optimization of another. For instance, a sample may need more or less cross-linking...Sonication (or enzymatic digest) will also have to be optimized for both tissue or cell type, amount of protein...length for your output assay. A longer sonication time could mean needing to increase crosslinking to counteract...

  6. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...again, and the same goes for your workflow. Next time you make a mistake, instead of silently vowing never...rushing, no matter how hard I tried to build in extra time before the experiment. What fixed it was not trying...fields, and military bases, checklists are the ultimate tool for doing things accurately. Thankfully, ...You may find that after running a protocol a few times, you no longer need the full checklist. Great! But...doing, and doing well, so make sure you’re taking time to do them carefully and thoroughly. Have a physical... things legible. It is faster to spend more prep time on an experiment than it is to rerun an experiment...

  7. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...interest, it may be time to look at the indirect approach - which means it’s time to talk about secondary...technique, and sometimes each individual assay, will need to be developed and optimized for the samples...that a trusted stock of secondary antibodies saves time and money when developing assays.  Protocol architecture....  Even though the secondary antibody incubation time is often shorter than that of the primary antibody... to take place over multiple days. Increased run time is considered one of the major drawbacks of the ...attaching to a single primary antibody, it is sometimes assumed that the direct method is more quantitative... the direct or the indirect method.  New Methods Time is a precious commodity in the lab and eliminating...

  8. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...proteins for live cell imaging can be excited many times, thereby producing a large number of emitted photons...number of excitation/emission cycles per unit of time, decreasing the likelihood that a fluorophore will...are excited with very intense light (for a brief time), whereas in widefield imaging fluorophores are ...with relatively low light levels (for a prolonged time). Other illumination strategies, such as 2-photon-excitation...performed that measures fluorescence intensity over time. In order to predict how fluorophores perform in...in ‘real experiments’, it is advisable to perform time-lapse imaging of cells producing the fluorescent...comparing how the fluorescence intensity changes over time, the photostability of the fluorescent proteins ...

  9. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ... five rules. By 2020, they had used this data-optimized assembly design (DAD) to build out three tools...you. Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments...Table 1).   Enzyme Temperature Incubation time/cycling Overhang length T4 DNA ligase 25...incredibly complex reaction possible, NEB also optimized the thermocycler protocols and made sure that ...complex reactions benefited from longer incubation times and didn’t necessarily require cycling. The Pros...assembly types, circular assemblies yielded 500 times the number of plaques than the linear ones. The ...selection process and benefited from a longer reaction time of 15 hours. This suggests that 52 fragments may...

  10. Tips for Technical Support Calls

    Type
    Blog Post
    ... utility in the least amount of time. Though I now spend far less time answering help emails and phone...call. Though my experience is based solely on my time at Addgene, I’m confident that these tips and tricks... call is a big commitment when you’re busy. Your time is precious. Here are a few things to consider before...with your product - this could save you a ton of time. I've definitely thrown out all the paperwork associated...know was just buried beneath someone's lunch. Many times, I’m pleasantly surprised to discover that the most...We understand how exasperating it can be to lose time and money on a potentially faulty product. Please...response, however, consider following up. Ask for a timeline if you are not given one. Though an initial response...

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...ahead of time!). This notice can be shortened as the meeting settles in and people get the time recurrently...bench I certainly had concerns that I may not have time to stay current or enjoy that discussion. Luckily...specific field(s). Most anyone who has spent any time in academia has likely had the opportunity to attend...that occurred once a week for several months at a time during the spring and fall semesters. Our scheduling...for the first meeting so that people can set aside time in their schedules and read the paper (PS: All participants...successful meeting: a clear purpose and effective time management, for starters.  Now, you are ready for... for scheduling conflicts. This helped us find a time that accommodates more people. The feedback could...

  12. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...contribute to my community. This past year, I spent some time thinking about how I could combine all of these ...event-planning base and will know how to reserve space, time events, and network for volunteers. Speaking of...this opportunity as coming from networking at the time, but that means it was the best kind of networking... a presentation for 8th graders was a little intimidating. Too simplistic and I would insult the students...does, and how one becomes a scientist. I gave a timeline of my science life, starting back to Mr. Kelley...communicators.  What can CRISPR do? I spent more time on this section of the presentation because, in ...Fig. 2). One thing I will likely change for next time is giving fewer examples and think of a way to make...

  13. The Challenges of Cell Culture

    Type
    Blog Post
    ...culture for an extended period of time (>10 passages or >3 months). Other times cell lines are unavailable from...concern for the discipline (or lack thereof) in times gone past but these days it’s never been easier ...biology. Engaging in this practice would seem to save time and money but cells lose provenance (this is the...closely followed by T24 bladder cancer cells. Estimates of the amount of money wasted in basic oncological...case if you obtain a cell line from a colleague. Time and money are wasted, papers are retracted and there...cells from a repository like the ATCC, there are times when it is important to check their identity. For...once you’ve passaged your current batch too many times or you suspect there is contamination. You would...

  14. Fostering communication in remote collaborations

    Type
    Blog Post
    ...and grant applications in real-time so they can provide input at the times that work best for them. This...challenges can be exaggerated when communicating across time zones, digitally, and asynchronously. The best way...collaboration includes scientists working in different time zones, it’s important that everyone can access data...communication of emailing documents to one person at a time. Just be sure there is a point person responsible... collaboration. Some things to figure out are timelines for experiments and manuscripts, who is responsible...reaching out if any milestones are missed. Spend time in person Presenting the results from my lab...circumstances permit, think about having collaborators spend time with each other in person. One possibility is to...

  15. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...Out of the genome Tips for a first time CRISPR User (by a first time CRISPR User) Additional resources...known. If a strong candidate emerges, downstream optimization is performed to take the drug to the pre-clinical...and can help weed out a lot of poor drugs before time and money gets put into them in those later stages...to inhibit, activate, or knockout many genes at a time to identify ‘hits’ - genes that affect disease progression...onset. These hits can reveal individual genes or sometimes even genetic pathways that are worth investigating...toxicity. This added QC is where CRISPR can save time and money to make sure quality candidates are selected...also been added to the CRISPR technology to allow time-controlled edits to occur, such as in the context...

  16. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...been requested 20+ times   A red flame means a plasmid has been requested 50+ times   A blue flame means...deposited plasmids have been requested more than 100 times each, thereby achieving the coveted “blue flame”...influential that it is requested from Addgene many times as the technology disseminates throughout the research...means a plasmid has been requested 100+ times Since we so sincerely appreciate our depositor’s dedication...Addgene that has been distributed more than 100 times. We hope these desktop trophies will serve as badges...can imagine, answering plasmid requests can be a time-consuming chore for labs that have popular plasmids...material transfer agreements, thereby freeing up time for our depositors to continue conducting research...

  17. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... so you can rely on nap times, after bedtimes, or before they wake up for time to do your own thing. Build...well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the Flowtime Technique... on. Practice self-care and stay connected Find time for exercise. Many studios and gyms are now offering...social support are even more necessary during these times of stress due to #COVIDー19. Receive and provide ... social media professionally but haven’t had the time until now.  Network. There’s a lot of networking...made for kids. Plan out meals and snacks ahead of time.  Find learning activities for kids. For example...

  18. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...the TSS but you can worry less about sequence optimality because you generally have fewer sequences to...so many possibilities, picking a gRNA with an optimized sequence is of primary importance (more on this...subsequent screening for successful edits. This is a very time consuming process and should not be undertaken lightly... for this technology, and the user may need to optimize numerous parameters to generate the desired edit...have a dozen or so gRNAs to choose from in the optimal location. It is also important to have good information...approximately equal importance in design – an optimized sequence will do little if it is in the wrong ...there are fewer gRNA to choose from, and thus an optimal sequence may not be available. Predicting gRNA ...

  19. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...irreproducible results to future work cost both time and money with estimates reaching in the billions of dollars ...apply these findings in their own work ultimately wasting both time and money. The obvious solution to ...lab and the entire research enterprise time and funds. It’s time to start putting them into practice!   ...program beneath it. You can jump to the different time stamps in the description to watch any sections ... body works, generating new therapeutics, and sometimes formulating new biological concepts. However, ...the final manuscript regardless of whether the ultimate results were positive or negative. I can feel ...of pre-registration doesn’t come to fruition any time soon, the Center for Open Science does host a platform...

  20. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    ...At the time when we spoke, Susan was helping cover social media while the Hutch hired a full-time social...directions is sometimes at odds with the space needed to be creative. “You need a lot of time to let you ...you mind process things. Sometimes finding that uninterrupted time to be creative is challenging,” says ...written.” Ultimately, Susan feels she was lucky to have been in the right place at the right time when she...research for a general audience. She then got a full time position with a Washington D.C.-based communications...
Showing: 161 - 180 of 927 results