...not right for the development of phage-based therapeutics. After a brief stint at the venture capital ...Novophage's focus. The company went from developing therapeutics to developing phage-based tools for detecting...develop tests for food products as opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens...for Dr. Koeris to transition from developing therapeutics to developing a diagnostic? 36:53 - 38:00 : ...Advice Learn How to Be A Good Manager from Addgene Executive Director Joanne Kamens Resources on the Addgene...

...2013). In vivo-directed evolution of a new adeno-associated virus for therapeutic outer retinal gene delivery...to Addgenies Brian O’Neill and Ina Ersing for contributing their expertise! References and resources References...epithelium after intravenous delivery by directed evolution of underexplored sites on the AAV capsid. Molecular... AAV5 Both Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic...Wagers, A. J., & Sabeti, P. C. (2021). Directed evolution of a family of AAV capsid variants enabling potent...

... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...how a virus enters the cell. Different serotypes utilize a number of different receptors, co-receptors, ... 2013, Figure 1. Used under Creative Commons Attribution License. Within the nervous system, remember... 2019, Figure 4. Used under Creative Commons Attribution 4.0 International License.   Inducible promoters...Konishi, H. (2014). A comparative analysis of constitutive promoters located in adeno-associated viral ...

...to light. Robert Campbell’s lab illustrates the utility of PhoCl in a variety of settings. For instance...in combination with this caging technique to beautifully demonstrate light-dependent Gal4 transcriptional...LSSmCherry1 & RDSmCherry1: Engineering and directed evolution of new RFP variants The toolbox of fluorescent...group’s PLOS ONE paper for more on the directed evolution of mCherry and the spectral properties of various...systematic examination of essential genes in vivo. Utilizing CRISPR interference (CRISPRi) technology, the ...xylose-inducible dCas9 paired with a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each...CRISPR tools for plants CRISPR inhibitors Bacteria utilize CRISPR Cas9 systems to defend themselves against...

...limited by its lower resolution. The combination of this method with superresolution microscopy may improve...improve the resolution, although background signal would be a concern. Another caveat of the study is the...the sensitivity of the technique. One potential solution is SunTag, a synthetic scaffold that can be used...chromosomal loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad...

...she started things off, “We are all capable of contributing to change.”  Check out Joanne's Reddit AMA ...of promise for science—we have powerful tools, beautiful questions, and smart scientists.  At the same ...audience to use their useful energy and to choose institutions with allies engaged in training excellence to...Incentives and Funding.  I can’t wait to see what solutions these talented postdocs will come up with to push...must do science to be happy so the beginnings of solutions can’t come too soon.  Watch the Future of Research...

...FLEx vector design Recombinase-dependent vectors utilize pairs of specific sequences, lox (recognized by...this occurs to alert the scientist to exercise caution when using them.    Figure 2. Schematic ...post-translational modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018)....to transduce your cells of interest. Using a constitutively expressing vector for this step may save time...0.13% respectively) still required a 200-300 fold dilution to minimize off-target expression. Since recombination...vary between every lot of AAV vector, a range of dilutions and/or injection volumes may need to be tested...

...numerical risk allowance in a statistical test. Distribution - spread of data; typically, normal (bell curve...data.     Fig. 2: Examples of data distribution profiles.   Parametric tests  Statistical... as follows:   Parametric test assumptions: Distribution – Normal Variables – Independent Variance –...assumptions for the tests described above (normal distribution, equal variance, and independent variables),...in that variance. If you want to know type of distribution you are working with, graphing data with a histogram...relative to quantiles derived from a theoretical distribution. If your data is normally distributed, it will...

... cleverly designed experiments and innovative solutions necessary for advancing the field.   What ...antibiotic resistance gene. In contrast, plasmids utilized in the lab are usually artificial and designed...gene of interest into a vector, scientists may utilize one of a variety of cloning methods (restriction...cost-effectiveness enable molecular biologists to broadly utilize plasmids across a wide range of applications. Some... viruses that can be used in research or for therapeutics Addgene has compiled various educational resources...

...Addgene! As part of our new Viral Service, we’re distributing lentivirus (with many CRISPR tools included ...your research.   Why distribute virus? Plasmid distribution is booming, but, over the years, many researchers...them share and distribute other reagents. Virus distribution came up time and time again with many researchers...with light will be available soon. We’re also distributing AAV preps with particular serotypes that can...resources for future users. We’re excited to be distributing new types of materials. At the time of publication...

...operations for the coming week. In an abundance of caution we are going to go into ‘snow day mode’. That is...shut-down and the subsequent fluctuations in distribution. I don’t have space in this blog to call ...scientific community.  With the dramatic reduction in distribution, we had to figure out a way to maintain our ...especially proud to support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating... cloning grade DNA service, and viral vector distribution is recovering from the pandemic-related slowdown...

...requesting institutions safeguard themselves from undue liability. Before Addgene can begin distributing a material...materials? If so, you should always talk to your institution’s TTO or legal office first. Generally, Addgene...points for broaching this subject with your institution’s tech transfer professionals: Describe to your...plasmids) among universities and other research institutions TTO: Technology Transfer Office. Some synonymous...

.... Stochastic multicolor labeling is a powerful solution for discriminating between neurons for light microscopy-based...copies per cell per color, following a Poisson distribution. To overcome the limited expression levels of...PMCID: PMC3713494.  Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal circuitry with an index-optimized...and index matching of tissue samples for High-resolution fluorescence imaging using SeeDB2." Bio-Protocol... et al. "Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis...

... Although isotype conversion is routinely used to increase the utility of recombinant antibody tools, ...converted, or chimeric, antibodies may be the right solution for you. Read on to learn more. What is an isotype...reduce immunogenicity and thereby enhance the therapeutic potential of antibody medicines. In this process...post has answered all of your questions about the utility of isotype conversions and chimeric antibodies!...

...investigation of this pathogen. The sharing and distribution of cell lines from repositories on opposite ... 2 weeks. This is a striking example of the contributions that scientists are able to make when they have...The Jackson Laboratory, a nonprofit research institution, has been able to rapidly propagate this strain...around the world. This scale of production and distribution is possible due to the dedicated facilities ...repositories’ capacity for storing, validating, and distributing such reagents are all ways in which the scientific...

...reprogramming tumor-associated inflammation for therapeutic benefit ”as stated by the organizers. My overall...immunosuppressive reaction in order to improve therapeutic outcomes.  If you are studying inflammation and...doctoral candidate at Binghamton University! Cancer therapeutics The so called field of Immuno-Oncology, a sizzling... hot topic, includes studies on novel cancer therapeutics such as  (Chimeric Antigen Receptors) CAR T ... to find out more about our free deposit and distribution service! References: 1. Pietrocola, Federico...

...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...Addgene’s largest new initiative since we started distributing plasmids in 2004. We’ve already distributed ...lead on operationalizing viral production and distribution, this project involved every Addgenie in the... to enable efficient, tracked production and distribution of viral samples. The Office Team made sure ...permission from relevant plasmid depositors and their institutions to distribute viral preps derived from plasmids...

...neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE) dCas9-SSAP with reduced error...neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE) by: Brian O'Neill  The Viviana...multiplexed version of the Cre-mediated AAV Targeted Evolution method (M-CREATE), in which one applies both positive...likely to afford the ability to deliver more therapeutics to the CNS, and with reduced systemic side effects...    Fig. 2: Summary of the generational evolution of the AAV capsids (left) and transduction patterns...

...within an E. coli cell, but vectors can be widely utilized across many different cell types and each one ...of these vectors. S. pombe plasmids oftentimes utilize an ARS to aid in high transformation efficiency...markers for yeast Historically, scientists have utilized auxotrophic selection rather than antibiotic selection...auxotrophic strain/ selection element pairs must be utilized or a new combination needs to be created in advance...improvements in antibiotic selection have made utilizing the more traditional drug selection methods feasible...

...irreproducibility in science or even to discuss potential solutions. Despite all the talk, not much was being done...present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing and were...continuously talking about a problem and potential solutions, take actionable steps - In the case of this collaboration...people at the right time. Be inclusive and seek solutions by working with communities, not for them - Another...conferences this year. Workshops are confirmed for the Evolution meeting, ASM meeting, and IS-MPMI. If you’re interested...