We narrowed to 315 results for: bon;

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   ...exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base editor...

2. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...promoters were also tested in different plasmid backbones that varied in size or carried different genes...

3. Lighting Up Cell Signaling with Photoswitchable Kinases

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   ...photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable...

4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

5. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   ...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

6. CRISPRainbow and Genome Visualization

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   ...Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ...

7. Developing Lab Management Software for Biology

   Type

   Blog Post

   ...plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you simply don't have enough context...

8. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   ...CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage...

9. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ...disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations N497A, ...

10. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...things like butanol - very simple molecules - a few carbons and oxygens. Nothing too much more sophisticated...

11. New England Biolabs Cell-Imaging Plasmid Collection

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    Collection

    ...Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133...localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression ...localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...Mootz HD, Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. ...

12. Penn Vector Core Partnership with Addgene

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    Collection

    ... Vincent Pieribone AV-1-36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473...mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-50943 50943-...mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68717 68717-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68719 68719-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68720 68720-...Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-PV2723 98929...pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-PV2521 105551...

13. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    ... make your own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed...

14. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post

    ...gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin resistance...

15. Hot Plasmids February 2024

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    Blog Post

    ...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...

16. Viral Vectors 101: Chemogenetics

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    .../10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan ...

17. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 Adjust pH ...

18. Hot Plasmids and Viral Preps - March 2021

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    ...reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples of potential...

19. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post

    ...it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 characters!...

20. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ...at the Faculty of Sciences of NOVA University in Lisbon, Portugal. She leads the Glycoimmunology group ...