...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...’s because MoClo specifically refers to the organizational schema described above, and not any particular...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...

...Approval 1+ business days, varies by requesting organization Viral vector preps remain subject to the terms...

... available in your lab or organization, and approvals from the organization overseeing your work. Good...the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular choice for administering...and to avoid potential off-target pokes to nearby organs. Restraint: scruffing Location: peritoneal cavity...Part 1 Mouse Modeling Part 2 Five Popular Model Organisms Additional resources on Addgene.org Genome Engineering...

...your organization Public Speaking Time management Managing a team Conflict resolution Organization Leadership...than you thought! When change happens at your organization, when your boss needs someone to step up, when...likely collected some great time management and organizational skills. If you are responsible for working ...students, managing inventories in the lab, or organizing a research project, one can assume that you’ve...

...variety of model organisms. Use the links below to find Gateway plasmids for your organisms of interest: ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning.... A Genetic Switch: Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources...

...RNAi in a wide range of cell types, tissues and organisms Luk Parijs 11619 pLB Modified pLL3.7; Genetic ...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...www.taleffectors.com) . Whether you work in a C. elegans lab and have been struggling to mutate a specific...

...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...panel was that there are many companies and organizations (including Addgene), that need people who are...questions, panelists and participants of the symposium began providing means to answer some of these questions...

... not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been developed...

... this genetic material to specific cells in an organism. Figure 1. Using viral plasmids to generate virus...

...senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone crazy...contribute to the Management Team that steers the organization. As an Addgene vet familiar with the expertise...like-minded group of co-workers who want to make our organization as environmentally friendly as possible. We ...make Addgene the most environmentally friendly organization on the planet, in which case whoa! Mission accomplished...Management Skills Volunteering at a Professional Organization Resources on Addgene.org Check out Career'...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

... on the advisory board for Art in Giving, an organization that raises money for childhood cancer through...art. About two years ago, I had a big role in organizing an Art in Giving think tank event where we brought...proposal, which Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful...wonderful!  I also volunteer for a dog rescue organization where I foster dogs and help screen dog adoption...

... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...directly to your institution’s MTA coordinator. Organizations that have opted into our eMTA system can even...

...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... making use of the new optogenetic tools in C. elegans and in Plasmodium. For those who do want to apply... Kendall Morgan...

...research is vital in combating misinformation and propaganda that limits access to full reproductive health...-based policy and legislation hit home hard in Michigan, where programs like the Great Lakes Restoration...planet. And as we rallied on the University of Michigan campus speakers thoughtfully reminded us to use...a postdoctoral researcher in the University of Michigan Department of Astronomy. She studies how planets...I was also incredibly impressed with how well organized the march was, as well as the friendly atmosphere...

... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...