We narrowed to 243 results for: grn

1. Plasmids 101: Screens vs. Selections

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   Blog Post

   ..., genome-wide knockout screens are possible with gRNA libraries targeting all known genes. This is a perfect...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   ...Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the...

3. How to Keep a Lab Notebook for Bioinformatic Analyses

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   Blog Post

   ...for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...

4. dTAG - You're it!

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   Blog Post

   ...CRISPR plasmids for protein tagging Find validated gRNAs ...

5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   Blog Post

   ...genomic locus in any cell type. By varying which sgRNAs are used with FIRE-Cas9, experiments can be designed...

6. CRISPR 101: Cas9 vs. The Other Cas(s)

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   Blog Post

   ...good reason. It is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it ...

7. Popular Retroviral Vectors and Their Uses in Scientific Research

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   Blog Post

   ... the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher...

8. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   ...variable portion of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists...

9. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   ... system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA without cleavage until...

10. Hot Plasmids and Viral Preps - January 2021

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    Blog Post

    ...is mediated by recruitment of MS2-functionalized gRNAs.  Find the yeast CRISPRa plasmids here Reduce ...

11. Hot Plasmids: Winter 2025

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    Blog Post

    ...of PE protein components (Figure 1), an altered epegRNA delivered with an enhanced pU6cm promoter, viral...

12. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...dedicated to expressing Cas9, while the other houses the gRNA and scaffold. If you don’t feel like doubling the...

13. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post

    ...interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ...

14. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...transfect/inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA- and Cas9-expression...

15. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine...

16. CRISPR Antimicrobials

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    Blog Post

    ...Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ...

17. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post

    ...protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene...

18. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ...Replication What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go...

19. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    ...Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled libraries at Addgene...

20. CRISPR-mediated Plant Base Editors

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    Blog Post

    ...expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers...