We narrowed to 245 results for: grn

1. Improved Plasmid Maps Powered by SnapGene

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   ...commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display, you...

2. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   ...with CasRx and either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating...

3. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ...Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the...

4. Plasmids 101: Screens vs. Selections

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   ..., genome-wide knockout screens are possible with gRNA libraries targeting all known genes. This is a perfect...

5. How to Keep a Lab Notebook for Bioinformatic Analyses

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   ...for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...

6. dTAG - You're it!

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   ...CRISPR plasmids for protein tagging Find validated gRNAs ...

7. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...genomic locus in any cell type. By varying which sgRNAs are used with FIRE-Cas9, experiments can be designed...

8. CRISPR 101: Cas9 vs. The Other Cas(s)

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   ...good reason. It is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it ...

9. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ...variable portion of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists...

10. Hot Plasmids and Viral Preps - March 2021

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    ... system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA without cleavage until...

11. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ... the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher...

12. Hot Plasmids and Viral Preps - January 2021

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    ...is mediated by recruitment of MS2-functionalized gRNAs.  Find the yeast CRISPRa plasmids here Reduce ...

13. Hot Plasmids: Summer 2025

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    ...target DNA, recruited by either the ωRNA (TnpB) or a gRNA (Cas9). Drawings not to scale. Created with BioRender.com...

14. Hot Plasmids: Winter 2025

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    ...of PE protein components (Figure 1), an altered epegRNA delivered with an enhanced pU6cm promoter, viral...

15. The AAVantages of AAV in CRISPR Screens

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    ...dedicated to expressing Cas9, while the other houses the gRNA and scaffold. If you don’t feel like doubling the...

16. Plasmids 101: Knockout/Knock-In Plasmids

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    ...interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ...

17. Google Forums Round Up: First Impressions of NgAgo

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    ...transfect/inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA- and Cas9-expression...

18. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene...

19. CRISPR 101: RNA Editing with Cas13

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    ...edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine...

20. CRISPR Antimicrobials

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    ...Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ...