We narrowed to 243 results for: grna

1. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   ...Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the...

2. Plasmids 101: Screens vs. Selections

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   Blog Post

   ..., genome-wide knockout screens are possible with gRNA libraries targeting all known genes. This is a perfect...

3. How to Keep a Lab Notebook for Bioinformatic Analyses

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   ...for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...

4. Neurodegeneration Plasmid Collection

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   ...75637 EIF2AK2 gRNA (BRDN0001146342) EIF2AK2 hU6 VWM disease David Root 75638 EIF2AK2 gRNA (BRDN0001148577...75639 EIF2AK2 gRNA (BRDN0001148668) EIF2AK2 hU6 VWM disease David Root 76195 ERBB4 gRNA (BRDN0001162372...David Root 76196 ERBB4 gRNA (BRDN0001149467) ERBB4 hU6 ALS David Root 76197 ERBB4 gRNA (BRDN0001146197) ERBB4...David Root 76198 ERBB4 gRNA (BRDN0001162324) ERBB4 hU6 ALS David Root 76361 TBK1 gRNA (BRDN0001145663) TBK1...David Root 76362 TBK1 gRNA (BRDN0001148392) TBK1 U6 ALS David Root 76363 TBK1 gRNA (BRDN0001148607) TBK1...David Root 76470 GAK gRNA (BRDN0001146687) GAK U6 Parkinson's David Root 76471 GAK gRNA (BRDN0001146263) ...David Root 76472 GAK gRNA (BRDN0001148048) GAK U6 Parkinson's David Root 76809 PANK2 gRNA (BRDN0001149459)...

5. CRISPR 101: Cas9 vs. The Other Cas(s)

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   Blog Post

   ...good reason. It is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it ...

6. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ... the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher...

7. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   ... system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA without cleavage until...

8. dTAG - You're it!

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   Blog Post

   ...CRISPR plasmids for protein tagging Find validated gRNAs ...

9. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   ...variable portion of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists...

10. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post

    ...interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ...

11. Hot Plasmids and Viral Preps - January 2021

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    Blog Post

    ...is mediated by recruitment of MS2-functionalized gRNAs.  Find the yeast CRISPRa plasmids here Reduce ...

12. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post

    ...than relying on a two-component system dependent on gRNA design (SAM) or peptide design (SunTag). This streamlines...(SAM) Description SAM uses specially engineered sgRNAs to increase transcription. This is done through...

13. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...transfect/inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA- and Cas9-expression...

14. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    ...Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled libraries at Addgene...

15. Hot Plasmids: Summer 2025

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    Blog Post

    ...target DNA, recruited by either the ωRNA (TnpB) or a gRNA (Cas9). Drawings not to scale. Created with BioRender.com...

16. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...dedicated to expressing Cas9, while the other houses the gRNA and scaffold. If you don’t feel like doubling the...

17. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine...

18. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

    ...). Created with BioRender.com. Before using gRNAs in vivo, they should be screened for activity in...transcription at genomic sites targeted by specific sgRNAs. Its RNA-guided nature makes CRISPRa much simpler...all the protein and RNA components, and several sgRNAs must be screened to identify effective ones. Simplified...constrained by AAV packaging limits (i.e., how many sgRNA expression cassettes will fit). Cre-dependent expression...expression of the SPH activator paired with AAV sgRNA delivery make the SPH mouse an attractive tool to...Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs can be found here. Neuron-optimized viral vectors...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...

19. CRISPR Antimicrobials

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    Blog Post

    ...Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ...

20. Lentivirus Plasmids

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    Collection

    ...plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search "lenti...