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Showing: 161 - 180 of 265 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...viral vector production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-0716-2918-5_5 Haery, L.,...Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389/fnana.2019.00093...Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https://doi.org/10.1002/cpmb.8...

  2. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post
    ...CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen...Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang...CRISPR-Associated Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional...

  3. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post
    ...Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, ...blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635 Gilda, J....blots. Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j.ab.2013.05.027 Additional Resources...

  4. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman ...Introduction to Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy...validation of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources...

  5. Plasmids 101: Repressible Promoters

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    Blog Post
    ... Tet-On and Tet-Off systems: Second generation. https://www.takarabio.com/products/gene-function/tet-inducible-expression-systems...in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman...

  6. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post
    ...Mammalian Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová...Mycoplasmas Using Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, ...Cell Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi...

  7. Antibodies 101: Flow Cytometry

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    Blog Post
    .... Review of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow...Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le...recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources...

  9. Congratulations, Deck The Lab winners!

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    Blog Post
    ... #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com/OOrwo9feyJ — BTC...Remind them you care!#thanks #health #mentalhealth https://t.co/Td2M6AOpuW pic.twitter.com/ofLI4eD8Zs — Microbigals...

  10. Reflections and Looking Ahead

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    Blog Post
    ...for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A....reproducibility in biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...

  12. Antibodies 101: Affinity Tags

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    Blog Post
    ...Protocols in Protein Science. John Wiley & Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J. D., & Waugh...Protocols (Vol. 205, pp. 99–118). Humana Press. https://doi.org/10.1385/1-59259-301-1:99 Resources on ...

  13. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...eukaryotic proteome? Open Biol. (2019) 9:180241. DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al.,...coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene...

  14. Hot Plasmids and Viral Preps - July 2021

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    Blog Post
    ... human disease.     Sun, et al. bioRxiv. 2021. https://doi.org/10.1101/2020.05.04.076067    Novel fluorescent...Chesterfield et al., ACS Synthetic Biology 2020. https://pubs.acs.org/doi/10.1021/acssynbio.0c00192   ...

  15. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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    Blog Post
    ...Review of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803...Chemogenetics. In Methods in Enzymology (pp. 181–196). https://doi.org/10.1016/bs.mie.2018.01.022 Additional ...

  16. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... GPCR transducerome. Nat Chem Biol 16:841–849 . https://doi.org/10.1038/s41589-020-0535-8 Takeda S, Kadowaki...human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional...

  17. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ...The Immunoassay Handbook (pp. 245–265). Elsevier. https://doi.org/10.1016/b978-0-08-097037-0.00017-8     .... 269, Issue 17, pp. 13048–13055). Elsevier BV. https://doi.org/10.1016/s0021-9258(18)99982-5 More resources...

  18. Hot Plasmids and Viral Preps - September 2021

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    Blog Post
    ...      Moore et al., ACS Synthetic Biology 202. https://doi.org/10.1021/acssynbio.0c00581   New tool ...Brookhouser, N., et al. BMC Biol 18, 193 (2020). https://doi.org/10.1186/s12915-020-00929-7    The CRISPR...

  19. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski... Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276 Additional resources...

  20. Addgene Depositors Get More Citations

    Type
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    ...research. View her research on her SSRN Author page: https://ssrn.com/author=1232466.     Neil Thompson is ...control trials in innovation. Visit his website at: http://www.neil-t.com/ .   References 1. Cong, Le, et...
Showing: 161 - 180 of 265 results