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We narrowed to 302 results for: human dat

Showing: 161 - 180 of 302 results

  1. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...whether the client invests substantial monetary and human resources towards the development of a new therapy...the business of life sciences and it's impact on human health.     Resources on the Addgene Blog Learn... and help resolve it through well-informed recommendations. Healthcare consulting firms that specialize...designing a strategic vision for a portfolio of candidate drugs. A decision like that would allow the client...especially helpful since they both provide up-to-date information about the industry, recruiting processes...

  2. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...whether or not your study is aimed at developing human therapies. If a therapeutic is the end goal, it ...that your results are viable for translation to human patients. Picking receptors The receptor you choose...written by Maya Peters Kostman in May 2020 and updated by Jason Nasse in July 2023.  References and Resources...

  3. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...happens within the first six hours. When added to human cells six hours after the introduction of Cas9 RNPs...Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...

  4. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute...during integration of transfected DNA into normal human cells."Nucleic acids research 18.9 (1990): 2733-...check out our viral vector guide pages to get up-to-date tips for your virus experiments. References 1....

  5. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for ...R. K., Doudna, J. A. Enhanced homology-directed human genome engineering by controlled timing of CRISPR... to identify knock-in clones by reading up on validating genome edits to find screening strategies for...

  6. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...generate in-house) a pre-validated antibody for each target protein. To study 20,000 human proteins, this would... a minute. As of now, the tool is available for human and mouse genomes only and deals with C-terminus...Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent tagging...In the light of these results, we re-analyzed our data and found that 75% of our highest knock-in efficiency...

  7. Neurodegeneration Research Collection

    Type
    Collection
    ...astrocytes. Endo et al Science. 2022 Nov 4. Base edit human mitochondrial DNA using mitoBEs . Yi et al. Nat ...new window) Michael J Fox Foundation (Link opens in a new window) A foundation dedicated to finding a cure...Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource page ...will advance more quickly with the avid sharing of data, tools, resources, methods, and results that are...living with Parkinson's today. The Michael J. Fox Foundation has made a number of tools (Link opens in a new...page . CHDI Foundation (Link opens in a new window) An organization with a mission to rapidly develop therapeutics... the progression of Huntington’s disease. The foundation offers curated information on tools and reagents...

  8. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...specify what species it is. Example: ‘h’ is for Human (homo sapiens), ‘m’ is for mouse (mus musculus),...commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include information about...

  9. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... R. J. (2022). AAV vectors: The Rubik’s cube of human gene therapy. Molecular Therapy, 30(12), 3515–3541... virus as a delivery vector for gene therapy of human diseases. Signal Transduction and Targeted Therapy... AAV can be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have...

  10. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...viral vectors and improved alternative methods." Human Gene Therapy, Part B: Methods 23.1 (2011): 1-7. ...adeno-associated virus type 2 Reference Standard Material." Human gene therapy 21.10 (2010): 1273-1285. PubMed PMID...concentration. We regularly make and validate fresh plasmid standards. We validate our absolute quantification...preparations. Addgene has used this method to generally validate our viral vector preparation protocol, and we ...around the world and can be used by researchers to validate their qPCR assay (Lock et al., 2010). In addition...targets: from variable nonsense to publication quality data." Scientific Reports 7 (2017). PubMed PMID: 28546538...

  11. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...scientists to identify the proteomic map of the human mitochondrial matrix (Rhee et al., 2013) and determine...ligase (Kim et al 2016). BioID2 consists of a humanized biotin ligase from A. aeolicus, which lacks the...proteins is hard to imagine yet alone study. To elucidate these interactions scientists have long used antibody...labeling (Rhee et al., 2013). APEX catalyzes the oxidation of biotin-phenol to the short-lived biotin-phenoxyl...complementation (PCA) technique with proximity labelling to validate binary protein-protein interaction as well as ...Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions through Spatial Proximity...

  13. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes. Sci Rep...Zhang F (2019) Engineering of CRISPR-Cas12b for human genome editing. Nat Commun 10: .https://doi.org/... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...) is the smallest Cas9 ortholog characterized to date. Kim et al. successfully used CjCas9 with AAV to...

  14. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...variants have been identified in humans and cataloged in the ClinVar database. Previously developed genome...reverse transcriptase with a sequence optimized for human codons; additional nuclear localization sequences...of heteroduplex     ✓ ✓ ~ ~ ~ Human codon optimization Improve prime editor expression...Traditional sgRNAs are protected from cellular degradation by Cas9 binding, but pegRNAs have an extended... written by Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December 2024. References...

  15. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...the potential CRISPR technology can have on the human microbiome. You can consider the microbiome as a...and metabolic processes, occupying the gut. Many human diseases have connections to either specific organisms.... Engineering the bee gut microbiome Beyond the human gut, the Barrick lab has deposited plasmids that...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...may be functional in most Enterobacteriaceae. An update to this system, called pEcCas/pEcgRNA, allows it...addition of a uracil DNA glycosylase inhibitor and degradation tag on the fusion protein to limit the activity...

  16. March for Science

    Type
    Blog Post
    ...Advancement of Science) believe GMOs are safe for human consumption in comparison to 37% of the general ...scientists believe climate change is mostly due to human activity while much less of the general public agrees...help support or reject a hypothesis. Looking at the data, scientists then revise their hypotheses and the... be useful to governments, however, inconvenient data must not be ignored. Returning to an earlier analogy...lose trust in scientists if the march highlights data supporting unwanted results (14). Some loss of trust...pandered to when strong or overlooked when small, but data is not going change depending on the number of people... as I believe people that can see and understand data themselves can come to conclusions themselves instead...

  17. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...used in human clinical trials. An off-target rate of 5% is far too high when considering human patients...likely will not have a significant impact on your data. You are trying to generate a knockout cell line...there are gRNA softwares available which score candidate gRNAs for on-target and off-target event efficiencies...them will also give you a specific list of the candidate off-target sites. For example, if there are 10... review of other targeted sequencing methods. Candidate site sequencing Sites with high sequence similarity...DNA binding sites within your experiment. For a candidate site-specific approach, mRNA quantification or...CRISPR 101: Cas9 The Other Cas(s) CRISPR 101: Validating Your Genome Edit          ...

  18. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3 mRNA binding factors...library even better, we would be very happy to share updated versions of the kit.” Reference Abil Z, Denard...

  19. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...show the gRNAs that were used for activation in a human system, search for ‘sapiens activate’ to filter ...many validated gRNAs in our newly curated Validated gRNA Target Sequence Table. What are validated gRNAs...searchable and sortable datatable that contains validated gRNA sequences. Validated in this context refers...effects are specific to that locus. The data in the validated gRNA table has been derived from scientists...containing the validated gRNA sequences alone. Sequence submission is welcomed for validated gRNAs that are...sequences here. Features of the validated gRNA table You can access the datatable here. Current table columns... exclude the data that you are not interested in. You can use the carats in the datatable headers to sort...

  20. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human Stem Cell Clones Detected by Whole-Genome Sequencing...Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids Research...approaches, and provide updates on how best to design gRNAs. You can also find validated gRNAs for your next...next experiment in Addgene's Validated gRNA Sequence Datatable. A more extended discussion of these subjects...Different databases annotate the TSS in different ways, and it has been shown that the FANTOM database, which...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...rise to an off-target effect, there’s not enough data to fully predict which sites will and will not show...
Showing: 161 - 180 of 302 results