We narrowed to 997 results for: lars

...practice in molecular biology and can be used in a multitude of scientific techniques such as molecular cloning... region of interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics qPCR ...Protocol: How to Design a primer  Molecular Biology Reference Molecular Cloning Techniques ... chain reaction? If you have ever worked in a molecular biology laboratory you have likely done a polymerase...cloning or molecular diagnostics.       Steps of PCR The beauty of PCR is that it can amplify DNA using...quantity. End point PCR is most commonly used in molecular cloning, sequencing, and genotyping. It’s extremely...simply PCR, is used for an array of different molecular biology techniques and experiments. End point ...

...whole tissue (15).  CLARITY is also compatible with LSFM, a method termed COLM (clarity-optimized light-sheet...seen some major advances in light microscopy, particularly related to the neurosciences.  These developments...transparent are enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented... tract or CST traversing pons and medulla, bp= basilar pons, NST nucleus of the solitary tract.  The arrows...light using a different objective lens oriented perpendicular to the plane of illumination (i.e. light sheet...multiple-view reconstruction (5). In order to image large tissue such as cleared rodent brain, the Ultramicroscope...was developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive reviews...

...some downsides to working in a large commercial company. In my particular situation, communication between...can sometimes go unnoticed at large organizations. Another downside to large commercial companies which ...cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in the American system... a type of pediatric kidney cancer. This was particularly interesting as I had just completed my thesis...I changed jobs. I highly recommend taking on a similar position in your field of interest before you dive...apply to study a Master of Science (MSc) in Human Molecular Genetics to give me an edge over other graduates...samples in the lab, but I really struggled with the large scale data analysis, as this is increasingly reliant...

...partitioning machinery away from a larger, low copy plasmid and the larger, low copy plasmid is more likely...plasmids at Addgene Read our Molecular Biology Reference References  del Solar G, Giraldo R, Ruiz-Echevarría...Replication and Control of Circular Bacterial Plasmids. Microbiology and Molecular Biology Reviews 62(2): ...replication and blocking replication machinery (del Solar, 1998). As the copy number of the plasmid increases... replication in plasmids that contain them (del Solar et al., 1998). There are two models for iteron-based...machinery when plasmid copy number is high (del Solar et al., 1998).  In cases of compatible plasmids,...plasmids, incompatibility due to partitioning is similar to incompatibility due to having the same replication...

...involve cellular dysregulation) is the mitochondria. These organelles have their own circular genomes ...been clinically exploited for antiviral purposes similar to that of vaccines. If you know the sequence of... Fig. 1: Exogenous sources of dsRNA Cellular sources of dsRNA Transposable elements such as...While these molecules are clearly associated with cellular damage and dysfunction, it is unclear what the...result of bidirectional transcription of their circularized DNA. These dsRNAs are typically contained within... acid hybrids are always around. R-loops are particularly enriched in highly transcribed regions and repetitive...described above play distinct roles in promoting cellular processes and functions, but they also have a ...

...Investigations in Molecular Cell Biology Website. Many thanks to our guest blogger, Clare O'Connor! Clare O'Connor...biochemistry, molecular cell biology and genetics at Boston College. She is particularly interested in...This post was contributed by guest blogger Clare O'Connor an Associate Professor at Boston College. National...computational methods to identify genes by their similarities to genes in other species, but these studies...gene function wide open. What if two seemingly similar proteins have acquired divergent functions due ...strains are available. Then, staff or students clone similar sequences from a less-well studied organism into...confident that the gene from the test species has a similar function to the gene in the reference species. ...

...steps for lysing cells and removing cellular debris look very similar. However, keep in mind that kit components... model, or overall throughput needed. Large constructs – Large plasmids, cosmids, or BACs, can be tricky...from bacterial cultures is a core protocol in molecular biology. And while kit-free plasmid purification...quality preps, while other applications require large amounts of high quality DNA. Figure 1 illustrates... mammalian cell transfections can require quite large amounts of DNA (easily in the micrograms scale and...your plasmid; separate plasmid DNA from unwanted cellular “debris”; and then clean-up the plasmid DNA (Figure... Figure 2: Plasmid isolation workflows are similar across kits. Lyse your bacteria to release the ...

...on a particular DNA-sequence configuration (state) based on the identity and order of a particular set ...mutations, and environmental conditions drive certain cellular behaviors, but little is known about the order... may be used to better understand and engineer cellular processes that are influenced by temporally ordered...input to an RSM drives expression of a distinct large serine recombinase that recognizes its own attB ...reactions are irreversible. Once expression of a particular recombinase is triggered, its effects on the ...registers will likely not be homogenous for one state, regular PCR may not be very informative – each primer pair...fraction of registers that are amplified by a particular primer pair. Then, by leveraging qPCR data from...

...from the cellular debris. There are several popular DNA vectors that can be used to produce large amounts...The central dogma in molecular biology is DNA→RNA→Protein. To synthesize a particular protein DNA must first... post-translational modifications Learn more molecular biology in our Plasmids 101 eBook! Researchers...functions. Proteins can also be purified for use in molecular biology research (eg. polymerases and other enzymes...expression systems can be scaled up for production of large amounts of protein. Some drawbacks of insect cell...insect cells require demanding culture conditions similar to mammalian expression systems. Bacterial expression... answer. E. coli is definitely one of the most popular hosts for protein expression with several strains...

...one example, modular constructs generated with MXS-chaining were used to label subcellular structures in...assembling these modular types of constructs, and different methods are suited for particular downstream applications...enzyme-based). Well suited for the modular assembly of highly similar or repetitive sequences. Intermediate...structure and protein localization, as well as cellular processes like gene expression and cell migration...the fluorophore, and thus enabled labeling of particular structures (Table 1). Each construct was flanked...detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining gave...kb insert. Table 1: MXS Constructs for Labeling Cellular Structures Construct Fluorophore Excitation...

...shape. These AAV particles are often found with cellular exosomes, small membrane vesicles or microvesicles... - 150 nm diameter) that are secreted from the cellular membrane into the culture medium. AAV particles...inside the vesicle, which may also contain other cellular molecules such as proteins or nucleic acids. This...infect more cells (as demonstrated in different cellular environments such as retina or glioblastoma). ...to neutralizing anti-AAV antibodies. This is particularly useful for therapeutic applications in vivo ...vesicles secreted from producer cells also contain cellular macromolecules (e.g. proteins, nucleic acids) ...retina after intravitreal injection to transduce bipolar cells and photoreceptors (Wassmer et al., 2017)...

... of most molecular biology labs. Recent and rapid advances in technologies that permit large-scale creation... and PCR products are the bread and butter of molecular biology labs the world over. Scientists have traditionally...-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids from ...technique in our partnership with Addgene to sequence large numbers of plasmids. plexWell™ technology overview...sequential “pooled library prep”, in which a relatively large number of samples are subjected to a DNA-barcoding...normalization problem often arises from the fact that large numbers of individual input DNA samples (e.g. a ...measured in time and consumables, can be daunting for large numbers of samples. As mentioned above, one of the...

...Increasingly popular Optogenetics tools use light to alter cell physiology and molecular processes via...generating DNA molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a ...mScarlet variants have shown great performance in cellular functional imaging and in protein fusions. The...PhoCl between an enzyme and an inhibitor domain similarly allows for control of enzymatic activity. In addition...variants The toolbox of fluorescent proteins (FP) for cellular imaging is constantly expanding. Case in point...point - check out the growing list of popular plasmids in Addgene’s FP collection. Just last month, Robert...

...a pathway to inhibit to block the cellular response to a particular ligand (such as when a component of... are involved in a studied cellular behavior. This approach is particularly suitable for in vivo screens...Institutet, in Sweden. He has a particular interest in using molecular tools to study the immune system...leader of a research group at the Centrum for Molecular Medicine (CMM), Department of Medicine, Solna,...attended a great talk when I was a PhD student. In particular, one slide of the presentation made a lasting...screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by targeting...signaling pathway. For example, one could study the cellular response to a cytokine known to cause the up-regulation...

... scientist wants to know if an organism has a particular gene mutation, southern blotting can be used ... is observed. Today Southern blotting has been largely replaced by real-time PCR to answer the same experimental...Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time PCR has several...next-generation sequencing techniques to answer similar experimental questions (see below). Importantly...ddNTP to a synthesis reaction along with all four regular nucleotides results in the synthesis of DNA strands...generation DNA sequencing machines (9). Today, similar technologies, sometimes referred to as sequence...discoveries (5, 8). While newer techniques have largely replaced these, these techniques still have useful...

...case of MODAL (modular overlap-directed assembly with linkers) which brings modularity to overlap-directed...deposit process in 2014 because of its gain in popularity. Gibson assembly is well known for allowing easy...insert your gene of interest into a vector with a large tag at the N-terminus, but you don’t have the tag...fairly high, so there is usually no need to screen a large amount of colonies. Excluding the time it takes ...those where the vector that you want to use is very large, has a high GC content, contains a lot of repeats...for the use of Gibson assembly combined with the popular CRISPR technology and is described in a recent ...described recently (Jiang et al. 2015), in which very large fragments of the bacterial chromosome (up to 100...

...Endogenous eNcapsidation for cellular Delivery (SEND). They demonstrate SEND as a modular system for packaging...and specify a large intervening deletion. Not only can this method precisely program large genomic deletions.... pyogenes CRISPR/Cas9 system is that it is too large to be effectively packaged into AAV particles for...  with an active nuclease.  Read the paper in Molecular Cell Find the CasMINI plasmids Efficient CRISPR...plasmids   Selective endogenous encapsidation for cellular delivery of RNAs By Mike Lacy The retroviral-like...endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering gene editing tools into cells...(epegRNAs) protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency...

...from Christopher Grefen’s lab or the MoBiFC modular bimolecular fluorescence complementation toolkit from...available to assess protein-protein interactions. One popular approach is to fuse a protein of interest to each...Figure 1). This method is generally known as Bimolecular Fluorescence Complementation (BiFC). It can provide...proteins are split between strand 10 and 11 to yield large (1–10) and small (11) fragments. In several cases...Next, let's look at a round-up of some new and popular split-FP plasmids from our catalog to help you ...) for use with GFP(11) Multiplexed labeling of cellular proteins with split fluorescent protein tags. ...

...jumping into the discussion! Semantic Scholar Semantic Scholar is an AI-backed literature search developed...Finding research papers is not particularly hard. There are millions of them. The real challenge is finding...also set up alerts for new publications from a particular author. Saving papers Papers can be saved to ...accounts can help you find papers related to a particular topic or that were published by the lab down ...follow journals related to your field of research or larger journals that publish about a broad range of topics...Institute for Artificial Intelligence. Semantic Scholar analyzes publications and figures using machine...relevant to your search terms. Key features Semantic Scholar has several ways to focus your search results. ...

...different system (Issa et al., 2023). This can be particularly burdensome in the brain where many different...broad tropism (the ability of a virus to enter a particular cell type), but there are differences in tropism...using chemogenetic tools, where modification of cellular activity outside of your experiment site or circuit... offer the most flexibility for expression in a large number of cell types and are useful if you're going...several different systems or want to transduce a large number of different cells. Promoters such as chicken-beta-actin... Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase...vectors in the central nervous system. Frontiers in Molecular Neuroscience, 7. https://doi.org/10.3389/fnmol...