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We narrowed to 187 results for: lentiviral

Showing: 161 - 180 of 187 results

  1. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

  2. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service ...Ready-to-use Virus for Your Research Visit Our Lentivirus Guide Page ...

  3. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    ...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...

  6. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...

  11. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    ...focusing on my technical skills with cloning and lentivirus production, I didn’t really start to recognize...
Showing: 161 - 180 of 187 results