...Table 1 has a detailed list of the bacterial species these plasmids are compatible with.  Table 1: Non-...ability to establish and maintain itself within host cells.  The plasmid has multiple ori that are functionally...reduced metabolic load (Meyer 2009). Figure 1: Idealized examples of a narrow host range plasmid...for DNA cloning in Gram-negative bacteria. Gene, 70(1), 191–197. https://doi.org/10.1016/0378-1119(88)90117... N.J.), 765, 327–343. https://doi.org/10.1007/978-1-61779-197-0_19  Meyer, R. (2009). Replication and ...often have a restriction barrier as a defense mechanism, and they can degrade foreign bacteria through...eutrophus, Bartonella bacilliformis, Bordetella spp., Brucella spp., Caulobacter crescentus, Escherichia coli...

... investigate how the virus interacts with human cells. Addgene’s mammalian target plasmids express these...for SARS-CoV-2 entry into human lung epithelial cells (Biering et al., 2021). In their search, they found...packaging) and contain an IRES-Puro marker for stable cell clone selection. For more information on these plasmids...Bajic G, Harrison SC, Wesemann DR (2021) Memory B cell repertoire for recognition of evolving SARS-CoV-... reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from...depends on its spike protein to bind ACE2, the host cellular receptor. To more easily study the spike variants...With the pseudovirus, scientists can study the mechanisms of infection and how to interfere with it in ...

... Figure 1: (A) Components involved in each type of CRISPR-Transposon system. (B) Mechanism of RNA-guided... smaller cargos, and the ability to easily cure cells of the plasmid after integration. Figure ...into the mini-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically...CRISPR-associated Transposase) by the Zhang group. Mechanism of CRISPR-transposons One of the CRISPR-transposon...

...mismatched translation rates and potential effects on cell growth, even hosts producing alternative tRNAs may...inclusion bodies that will generally segregate with the cell pellet during purification procedures. Even if you...a means to fine-tune gene expression." Molecular cell 59.2 (2015): 149-161. PubMed PMID: 26186290. PubMed...inclusion bodies and is folding properly. References 1. Angov, Evelina, et al. "Heterologous protein expression...reasons include: Metabolic pressures - it takes cellular resources to produce tRNAs that recognize different... Maertens, Barbara, et al. "Gene optimization mechanisms: A multi‐gene study reveals a high success rate...

... at Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial...writing and hopefully increase your success rate. 1. Submit your grant early! First off, and perhaps the...involved so it’s important not to throw in the towel if 1 or 2 funding bodies say no. If possible, get feedback...research (either because of expertise, equipment, or excellence of the institution). Likewise, if you’re staying...

...identifying new genes required for stem and progenitor cell maintenance in planarians in Developmental Biology...junction complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology...two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze...research projects have also been proposed as a mechanism for increasing diversity and inclusion in STEM... his teaching style, with the former offering excellent training in improvisation and the latter making...

...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...medRxiv.org) in the past few years.   Fig. 1: Stand aside, CERN - it's a new age of accelerating...scientists communicate with each other: from formal mechanisms like publishing a research article, presenting...

... successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience,... optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance...is equally important to have a plan to get to mechanism. Do the necessary tools exist to tackle the problem...

...118). Humana Press. https://doi.org/10.1385/1-59259-301-1:99 Resources on Addgene.org Addgene antibodies...and yes, we have an antibody for that!   Fig. 1 - Diagram of His-tagged protein purification work ...purification, it will be expressed along with all other cellular proteins within the expression system (bacteria...amylose resin and also provides a visual detection mechanism via applications such as western blot and immunofluorescence...

...phosphorylated DNA termini to 3 ́-OH DNA termini (1-4). In vitro, ligases (notably T4 DNA ligase) are ... enzymology of DNA repair enzymes.     References 1. Shuman, Stewart, and Christopher D. Lima. "The polynucleotide...thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G., et al. "Cloning... ́-phosphate to the ligation). The structural/mechanistic reason for this differential is not known for... E., et al. "DNA ligases: structure, reaction mechanism, and function." Chemical reviews 106.2 (2006):...ligase bound to nicked DNA-adenylate." Molecular cell26.2 (2007): 257-271. PubMed PMID: 17466627. 25. Luo...

...challenges involved in plant synthetic biology. Challenge #1: Public perception of synthetic biology One of the...challenging to engineer a specific function in these multicellular organisms. However, the rewards are great if...Additional work has also identified and characterized mechanisms and components of the circadian rhythm in petunias...understanding of the genetic pieces underpinning these mechanisms – our project wouldn’t be possible. A second ...

...first foray into Reddit. Here are some reasons why: 1. Reddit has a large and active community of people...incentivize good mentorship: AMA mechanics: Be prepared! The mechanics of doing the AMA were interesting... training is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s incredibly...topics to the Addgene blog The blog has many other excellent posts on science career topics as well. Instead...