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We narrowed to 522 results for: pes;

Showing: 161 - 180 of 522 results

  1. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...size. Like CRISPR, there is a variety of small RNA types to choose from and can be catered for your specific...of different applications that involve different types of small RNA molecules, such as: Micro-RNA (miRNA...and piRNA can be found endogenously in many cell types, while shRNA and siRNA are synthetic constructs ... end_module_attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text...

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...antibody-peptide labeling; specifically, cognate peptide epitopes fused to the protein of interest are recognized...system, dCas9 is fused to a scaffold containing epitopes recognized by scFV antibodies fused to VP64 transcriptional...for inducible expression, loxP sites, H2B, P2A or PEST2D sequences. Several pre-constructed promoter-selection...AUUUA [5 consecutive]) and protein (C-terminal CL1 & PEST). These modifications ensure that any signal from...antibodies. Unfortunately, while there are many types of epitope tags (eg. HA, MYC, Flag), they can be...them to GFP, and compared them to popularly used epitopes tags such as HA and MYC in vivo. The authors found... between the POI and a TAP tag (a tag with two epitopes that can be used to sequentially purify the POI...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...brain cell types. Look for more of these interneuron-targeting constructs and other serotypes available...Lacy Targeting gene expression to specific cell types in the brain can be a challenging — but critical... to target and manipulate distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://...

  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...BioRender.com.   Optogenetic tools Types of control We will discuss three types of tools: light-gated ion channels... been developed to selectively study individual types of GPCRs with greater spatial and temporal control...’ll need to know (and understand!) the hardware types, construction, and testing used for delivering your...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...dicer-like enzymes (DCL) process the dsRNA into specific types of interfering RNAs.   RNAi vs. CRISPR: Complementary...easily identified and have readily observable phenotypes quantified. Fluorescent reporter-based RNAi constructs... RNAi plants. With a visual reporter of RNAi, phenotypes, such as morphological parameters, can be easily... for Rapid Identification of Loss of Function Phenotypes. Plant Biology 7:251–257 . https://doi.org/10.1055...

  6. Antibodies 101: Validation

    Type
    Blog Post
    ...proteomic data from several different tissues or cell types. If your antibody indeed binds to your target then... the manipulation. Additionally, not all sample types are amenable to genetic manipulation, nor can all...the results. Using antibodies against different epitopes on the same target reduces the likelihood that...antibodies that are predicted to bind different epitopes of this protein.   You perform flow cytometry...

  7. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...cancers are caused by persistent infection of HR HPV types, predominantly HPV 16 and 18.” HPV plasmids as positive...genes as positive controls in her PCR screens. She hopes that these screens will both identify individuals...also better correlate the presence of certain HPV subtypes with risk and disease severity. Individuals who...

  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...had taken out a few foldscopes and held an improv workshop. These paper microscopes work as conventional...conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron resolution). I ...

  9. Deep Dive: qPCR

    Type
    Blog Post
    ... are suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference... on plate space. For many cell lines and tissue types, appropriate reference genes (and supporting data...unwanted variation into your data. Try different types of pipettes and pipetting methods to find what’s... it up for larger or frequently analyzed sample types and sets.   🔥Hot Tip🔥 The ABI machines automatically...

  10. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...which let you lock and hide individual vectors (shapes defined by 2D points connected by lines and curves...wide selection of tools for drawing and editing shapes and like Vectr, Inkscape uses vector graphics. ...settings let you tailor your search to particular types of images. For example, by selecting “Tools” and...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...affinity tags can make protein purification a cinch. Types of affinity tags, the proteins they work best attached...tag can be purified with several different resin types and is a fairly small tag that is unlikely to perturb...Affinity tags vs. other tags There are many different types of tags, including epitope tags (FLAG, Myc, etc....

  12. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...component for expression vectors: determines which cell types the gene is expressed in and amount of recombinant...also have selectable markers for use in other cell types. Primer Binding Site A short single-stranded DNA...wide range of applications. Some common plasmid types include cloning plasmids, expression plasmids, gene...

  14. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...publication for the specifics of your chosen plasmid. Types of biosensors When choosing a biosensor, you first...presence or changing cellular conditions. Other types of biosensors To focus on a particular gene or protein...the common fluorescent detection methods and the types of experiments they are commonly used for.  Table...

  15. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...mutated version of YGOI to test the different phenotypes of the specific mutations. For these purposes...effects.  And many more! We have a variety of other types of vectors for most applications used to study gene...

  16. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ... GENIE Project New viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li...Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor Marianne...eligible transfer plasmids and five different serotypes. Not sure what to request? Addgene's Data Hub ...

  17. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...profiles were then compared to that of reference cell types generated by the human fetal expression atlas. Computational...along with color coded nominated reference cell types.  (Adapted from Joung et al.)    Putting MORF...TFs which were predicted to generate unique cell types. Of the 24 TFs they tested, 22 expressed markers...

  18. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...in weeks Figure 1: Sending out 80 FedEx envelopes in a single day -- all while maintaining social...but we're planning to soon expand to other cell types, including more physiologically-relevant systems...Getting our research in line, with a row of FedEx envelopes waiting to be shipped out of the Krogan lab at...

  20. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...of course, many companies selling antibodies, microscopes, and dissection tools, but there were also people...means of delivering genes to many different cell types with minimal immunological effects. AAVs are used...in experiments targeting genes to specific cell types in the brain, mapping neuronal networks, and selectively...
Showing: 161 - 180 of 522 results