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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 208 results for: pme i

Showing: 161 - 180 of 208 results

  1. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...encoded magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start using.... Merkx recently applied this “trick” to the development of additional color variants. This resulted in...

  2. Clear and Accessible Writing

    Type
    Blog Post
    ...What is the goal, who is my audience, and what do I want to be different for them when I’m done? What ...online resources. On the backend, our product and development teams have worked hard in increasing and improving... 2023, we hosted our very first professional development workshop, “Make a Greater Impact Using Clear ...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...just six weeks later. The Zika publication timeline I think we can speak for all the authors on the paper...and rare, but severe, symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome...health emergency and called for the fast-tracked development of diagnostics. Mostly transmitted by Aedes mosquitoes...timescale. Our article ultimately reported the development of a rapid design and prototyping pipeline that...necessary to advance the technology through product development, the regulatory process, and manufacturing so...

  5. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...can later be verified by Sanger sequencing. How can I tell if my enzyme will cut? Whether performing a digest...Blocked by overlapping methylation Not affected PmeI Not affected Not affected Blocked by overlapping...

  6. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... follows are just a few suggestions based on what I have found to be especially beneficial during my own...scientists. Continuing professional development Professional development should be life long, and doesn’t...landing that next coveted job! Professional development (or lack thereof) One of the biggest challenges...programs lack any formal or mandatory professional development training. Further, even for those students and...take responsibility for your own professional development, not count on your institution, program, or adviser... a job fair, and utilizing your local career development center, if one exists. Get online! Review company... career development...

  7. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ... Shah et al., “Do you know the sex of your cell?” I can’t tell you how many researchers I’ve talked to...harm humans or contribute to failures in drug development – costs far greater than those of doing the research...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...of chemically converting adenosine (A) to inosine (I, treated as G by the cell). Surprisingly, the team..., 843–846. https://doi.org/10.1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B....pathways that were considered problematic in the development of CBEs and ABEs. This post includes a lot of...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...Addgene Viral Plasmids Which Type of Virus Should I Use? Resources on the Addgene blog Viral Vectors...scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common forms of lab accidents...

  12. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length version is strong...only in certain tissues or at certain times in development or there may be ways to turn them on or off at...tables above do not delve into the tissue or development-specific promoters available to scientists. Plasmids...

  14. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ..., Ishibashi, H., Yuasa, S., Takeda, S., Hasegawa, I., and Nakahara, K. (2011). Local and retrograde gene... J. M., Vandenberghe, L. H., Haute, C. V., Thiry, I., Deroose, C. M., Mortelmans, L., Wilson, J.M., Debyser...like these may be a starting point for future developments in the field of AAV vectors. Conclusion An ideal...intracellular transport of AAV could facilitate the development of more powerful AAV serotypes, and perhaps lead...

  15. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ... These Acr proteins were originally found in type I CRISPR-Cas systems.   Four years later, the Sontheimer...Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https://doi.org/10.1016/j.omtm.2018.04.008...

  16. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I with T74I (accelerated maturation in cells compared... such inhibitors have been characterized for type I CRISPR systems, but only recently have inhibitors ...is a red-shifted variant which, with further development, could be better suited for deep-tissue in vivo...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...doi.org/10.1016/j.ymthe.2022.09.015 Ramani, B., Rose, I. V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal... more comprehensive thanks to the consistent development and improvement of CRISPR-based technologies ...

  19. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (...-progress still in need of further study and development like MagLOV. The results with MagLOV raise broader...

  20. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...tools used in research, and data repositories. Below I describe how each of these kinds of connectivity facilitates... modern and fully supported API that enables development of custom integrations with other tools and resources...
Showing: 161 - 180 of 208 results