We narrowed to 395 results for: promoter

...Cas9 would have an off-target that lands in the promoter of another gene, thereby driving aberrant transcription... said, we generally pick guides by putting the promoter of the gene into a gRNA finder such as WU-CRISPR...

... pages, we added curation fields for purpose, promoters, expression types, and more, all of which help...How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to Verify Your Plasmid ...

...resistance cassettes (Prior et al. 2010), inducible promoters with different degrees of stringency and inducibility...Development of constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli ...

...following changes. In pCas9, we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon...pCutamp cures the plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed...

... of the people who participated as mentors or promoters were connected to that Tweet Up in 2013. These...connections brought in folks I didn’t yet know and promotion from those who could not participate but provided...

...the site of insertion and which can potentially promote tumorigenesis in vivo. The phenomenon has been ... PMCID: PMC2917235. 11. Nakagawa, M., et al., Promotion of direct reprogramming by transformation-deficient...

...screening direct interactions between drugs and promoters of oncogenes. He returned to academic research...

...sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary to add a ... start of my target sequence? A3: The human U6 promoter prefers a 'G' at the transcription start site ...of the crRNA) is also expressed from a separate promoter. In the pre-processing form, the crRNA contains...

...vector backbones, and gRNA structure, numbers, and promoters can all be chosen. Read the paper in PLoS One...

...practices. These concrete programs and practices to promote PhD professional development are intended to be...

...elements in plant expression cassettes: plant promoters, N-terminal tags, coding sequences of the gene...

...your plasmid – you carefully selected the optimal promoter for your gene of interest, painstakingly cloned...Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors Looking ...

...this system is tunable via a rhamnose-inducible promoter. While this system was optimized for Burkholderia...

...Chien lab (Kwan et al., 2007) and includes four promoters, six fluorophores with nonoverlapping emission...neurodegenerative diseases, the Cole Lab selected promoters for cell types directly linked to disease, including... components can be controlled by a variety of promoters for versatile and robust engineering. These components...

...AAV encoding part A of the transgene contains a promoter and the 5’-fragment of the transgene, followed... encoding part A of the transgene contains the promoter, the 5’ half of the transgene, a splice donor ...

...animals by using cis regulatory elements such as promoters and enhancers, multicistronic vectors, and post-translational...

...emotional response within the viewer, which could promote conservation efforts, as the subject matter is ...

...Blugene needs to be friendly, personable, fun, and promote our values of community and sharing.  One big feature...

...Common Features” function to find and annotate promoters, selectable markers, tags, origins of replication...

...relevant to your research interests. We do this by promoting citation, supporting sophisticated search, and...