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We narrowed to 395 results for: promoter

Showing: 161 - 180 of 395 results

  1. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...following changes. In pCas9, we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon...pCutamp cures the plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed...

  2. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... vector is inserted downstream of a functional promoter (gene traps). The resultant gene targeting system...pAAV-TK-Acceptor has a conventional heterologous promoter-driven TK-neoR gene. To modify these tagging vectors...pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances...

  4. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...transcription by placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores ... RNA Polymerase III. By expressing Corn under promoters for transcripts uniquely transcribed by RNA Polymerase...

  5. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...Cas9 would have an off-target that lands in the promoter of another gene, thereby driving aberrant transcription... said, we generally pick guides by putting the promoter of the gene into a gRNA finder such as WU-CRISPR...

  7. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary to add a ... start of my target sequence? A3: The human U6 promoter prefers a 'G' at the transcription start site ...of the crRNA) is also expressed from a separate promoter. In the pre-processing form, the crRNA contains...

  11. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...your plasmid – you carefully selected the optimal promoter for your gene of interest, painstakingly cloned...Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors Looking ...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...Chien lab (Kwan et al., 2007) and includes four promoters, six fluorophores with nonoverlapping emission...neurodegenerative diseases, the Cole Lab selected promoters for cell types directly linked to disease, including... components can be controlled by a variety of promoters for versatile and robust engineering. These components...

  16. Science as Art

    Type
    Blog Post
    ...emotional response within the viewer, which could promote conservation efforts, as the subject matter is ...

  20. SunTag and Fluorescent Imaging

    Type
    Blog Post
    .... More transcription factor binding sites in a promoter lead to higher transcriptional activation. Multiple...
Showing: 161 - 180 of 395 results