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Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Use this protocol to generate lentivirus

This protocol can be used to titer lentiviral preparations that confer antibiotic resistance.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

Addgene Executive Director provides advice on making the decision between academia and non-academia.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Learn about items from Addgene's viral service including lentiviral preps expressing SARS-CoV-2 proteins, ChR2 for targeted expression, DREADDs, GFP dependent Cre and FLPo, and more.

Scientists from UCSF identify 332 human proteins that the SARS-CoV-2 virus interacts with in cells in attempts to find druggable targets.

Learn about the fluorescent protein work being supported by the Michael Davidson and Rogers Tsien Commemorative Travel Awards.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Two techniques developed by Addgene depositors have made it possible to track mRNA translation at the single molecule level using fluorescent proteins.

Addgene Scientist Eric Perkins gives insights into the stem cell field he gained from the 10 years of iPSCs symposium

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Addgene's Advisory Board helps steer and promote Addgene in its goal of sharing science. We welcome CRISPR expert, John Doench, to the Advisory Board.

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

An overview of viral applications spanning from basic research science to clinical therapies.