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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 254 results for: tre promoter

Showing: 161 - 180 of 254 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...studying how that one protein interacts with that one promoter can tell you, it can be easy to get lost in the...Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts in the Microbiology Community Sign Up ...

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...changes were introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and...bacteria resistant to both spectinomycin and streptomycin, but could also be used for studying epistatic...

  3. Worm Expression Resources

    Type
    Collection
    ...recombinase from either a heat-shock promoter or a tissue-specific promoter and expression of the target FLP-out... from either a ubiquitous or a tissue-specific promoter. cGAL and Split cGAL plasmids - Paul Sternberg...Jorgensen Lab. A collection of plasmids that streamlines the production of targeting vectors for tag insertion...

  4. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox...nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ...K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural...

  5. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Viral expression cassettes typically contain a promoter (inducible or constitutive), your gene of interest...the last decade, viral applications in cancer treatment and gene therapy have also been in the limelight...approved the first oncolytic viral therapy in 2015 to treat melanoma, and many more are in the pipeline. Viruses...

  6. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...investigate the effect of regulatory elements — such as promoters, enhancers, or untranslated regions — on gene ...Cofactors/substrates Required Not required Signal strength Lower Higher Sensitivity Higher Lower Background...gene expression. The regulatory element is cloned upstream of the luciferase gene, and expression of luciferase...

  7. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ... 2008). Obligate dimers (such as dTomato) that promote interactions between the FPs interfere with native...Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced microscopy, University of Amsterdam...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced microscopy (University of Amsterdam...

  8. All in a Twist: dsRNA

    Type
    Blog Post
    ...-loops described above play distinct roles in promoting cellular processes and functions, but they also...production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing radiation, can also...

  9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...complex, to temporarily inhibit this process and promote copying of the edited strand to the unedited strand... co-express mismatch repair inhibitor MLH1dn Promote desired resolution of heteroduplex     ✓ ...non-homologous end joining. As a result, Cas9 treatment causes most products to be indels while the efficiency... In addition to the numerous innovations that streamline the process of prime editing, the Liu lab contributed...efficient as possible. Figure 2: Decision tree for selecting a PE6 variant. PE = prime editor; ... 2023) Prime editing has a lot of promise for treating genetic diseases. In our summer Hot Plasmids post...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm ...of two sgRNAs from independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for...along with 1-4 sgRNAs expressed from independent promoters. sgRNAs are cloned into separate vectors and then... assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one...pGGDestTol2LC vector. Uses five distinct zebrafish U6 promoters for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs...expression of two gRNAs from Drosophila U6:1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous ...Zhang Cas9/pTREX-n 68708 Other/Trypanosoma cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 ...

  11. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...several weeks which may also depend on transgene or promoter choice. Behaviors you can study using viral vectors...into the experimental setup, the mouse becomes stressed. It will likely move around faster in the following...some time to adapt to a testing apparatus. General stress levels of the test animal may also be reduced by...noise should be avoided as this will also increase stress levels and affect behavior. A fact to consider:... groups), health status, diet, cage enrichment, stress level, origin (vendor or in-house mouse breeding...

  12. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...nuclear localization, binding to the appropriate promoter sequence and activation of gene expression. In...bulk cell experiments, Clift et al. observed no stress or damage upon TRIM-Away, and the electroporated...re-adhered to the culture plate within 4 hr of treatment. In some primary cell lines, endogenous TRIM21...Listen to the Fire-Cas9 Podcast Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system...

  14. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...science and engineering. “Fix the Institutions” promotes gender equality in careers through structural ...factors may include cell type, the disease being treated, and other hormonal, immunological, and environmental...

  16. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...dopamine is an important neurotransmitter that promotes reward-motivated behavior, if the channelrhodopsins...as predict the channelrhodopsin’s photocurrent strength, wavelength sensitivity, and off-kinetics.   This...did not turn. Left-turning behavior in ChRger2-treated mice ceased when the light was turned off.  Together...

  17. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...gene expression and the selection of specific promoters may also help AAV vectors evade host immunity....potentially be treated by correcting the mutation or replacing the gene. A notable example is the treatment of Severe...conditions, opening up new avenues for therapeutics that treat disease at the molecular level. For instance, if...function. To date, gene therapy has been used to treat 10 infants with this disease. To do so, their T-...childhood onset of blindness and was thought to be untreatable until 2008. Following a single dose of subretinal...in vision over a period of 1 to 3 years – the treatment appears to be both safe and effective. Challenges...antibodies that neutralize the AAV vectors used to treat them. Scientists are working to overcome this issue...

  18. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...discussions. Get your voice out there. Use Twitter to promote your other science communication activities, e....engagement - Participate in science festivals and outreach If you are comfortable talking in public, volunteer...groups, charities and NGOs are often looking for outreach volunteers as well. 4) Blog - Hone your writing... a blog is not only a great form of scientific outreach, it also helps you to hone your writing skills...science communication. The National Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site ...

  19. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...incorporating it into a fourth plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated...experience of end-users, size of the transgene, and downstream applications should all be considered when choosing...factors such as resources available, cost and downstream applications. For example, while the PEG precipitation...be the best method when pure virus is required downstream. When assessing lentiviral titer, don't compare...
Showing: 161 - 180 of 254 results