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We narrowed to 535 results for: val

Showing: 161 - 180 of 535 results

  1. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...binding affinity (Janda et al., 2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions....2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects on Variable...articles/10.3389/fmicb.2016.00022 Torres, M., & Casadevall, A. (2008). The immunoglobulin constant region...

  2. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...Once drug candidates are isolated, they can be validated for specificity to their target, or their direct...effectiveness prior to patient treatment. Target validation When a target is known and a drug is developed... to measure the specificity of that target and validate the hit compound. The standard is to test the ...

  3. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...have become a popular model organism because their larval stage lends itself well to studies of neuroscience...startled by acoustic, visual or tactile stimuli, larval zebrafish react by performing a distinct C-bend...in a transparent animal: circuit function in the larval zebrafish." Current opinion in neurobiology 23.1...

  4. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...organizations that share our community-oriented values. Addgenies like the nonprofit company culture as...gain access to new materials and information. Core values drive decisions at Addgene If you’re ever curious...making process at Addgene, just look to our core values. These accurately reflect our culture and outlook...

  5. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...antibodies are both extensively published, knockout validated, and work in your application. But…they are the...differences are subtle, chimeric antibodies should be revalidated before use. In addition, chimeric antibodies ...resources on the Addgene blog Antibodies 101: Validation Antibodies 101: Polyclonal Antibodies Antibodies...

  6. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    .... The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand and a tyrosyl...different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands and releasing...overhanging 3´ deoxythymidine (T) residues that are covalently linked to topoisomerase. Vectors in these kits...

  8. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...Mentoring for Scientists series, you have: realized the value of having a mentor, developed some strategies for...Scientists Guide.  You can use it to clarify and discuss values. The activity is just the start – it is the discussion...inclusion, diversity, and open communication Feel valued by giving back Final tips for success Make mentoring...

  9. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...inaccuracies as they find them (anyone can register with a valid email address).  One may also simply attach important...frequently) for the sake of comparison or ease of retrieval.  These collection pages can be kept private, ... applications, a REST API allows programmatic retrieval of the majority of information in the database...

  11. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...all this information makes your manuscript more valuable to the research community. It also makes it more...research more reproducible and your results more valuable. Some journals, including F1000Research, also ...provide on data, materials, and methods, the more valuable your paper will be to the scientific community...

  12. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ..., to talk. That moment taught me an incredibly valuable lesson: Your presentation will be better if you...applies: what can go wrong, will go wrong. The trash removal service slams dumpsters outside the open windows...or present at lab meetings, you’ve developed a valuable skill set that will be useful in nearly any other...

  13. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...problem solving that I did in the lab has been invaluable in my more recent positions outside of the lab...how scientists can develop and highlight these valuable skills. Hopefully, having a better sense for what... you understand it. Step Two: Come up with and evaluate potential strategies to resolve the problem.  ...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...-15 kD but can have weak signals due to their monovalent nature. They are stable up to 80°C and can refold... affinity reagents, trendy as that may sound. Validated antibodies are still an excellent option for many... Exhibit Improved Tumor Lysis When Placed in a Divalent Tumor Antigen Binding Format. Journal of Biological...

  15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control  In...propagate your plasmids in: DH5α, NEB Stable, ccdB Survival, Pir1, or other. Whenever possible, we propagate...gene, such as Gateway vectors, choose the ccdB Survival strain. For plasmids that contain the R6Kγ origin...

  16. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...2) Public engagement - Participate in science festivals and outreach If you are comfortable talking in...in public, volunteer at science festivals. Or engage with non-scientists in an unusual setting: Discuss...making videos, presenting in pubs and at science festivals. Science communication is my way of living out...

  17. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...with a value of “0” indicating those markers are completely distinct from one another, and a value of “1...completely indistinguishable. This tool is of particular value when designing a panel.     Figure 3: Similarity...

  18. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...transcriptional and epigenetic regulation have been validated with these proteins. Furthermore, among those ...Addgene.org Check Out Our CRISPR Guide Pages Find Validated gRNAs for Your Experiments Get Ready-to-Use CRISPR...

  19. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...job well done. Your report will (needs to) feel valued. Talk to your manager and find a mentor Your manager...mentor - someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your...The latter will make you feel discouraged and not valued which will undoubtedly negatively impact your work...
Showing: 161 - 180 of 535 results