Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
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Blog Post
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Background: Argonaute and NgAgo
In eukaryotes, Argonaute proteins play an important role...searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute. They identified...
THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED... newest gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short...interference, binding RNA guides to cleave foreign RNA. Argonaute proteins are also present in prokaryotes, and ...invading DNA. In 2014, Swarts et al. characterized Argonaute from Thermus thermophilus (TtAgo), showing that...genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID...