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We narrowed to 15 results for: CFP

Showing: 1 - 15 of 15 results
  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...default RFP and CFP, while Brainbow 2.1 switches from the default GFP (green) to RFP, CFP, or YFP fluorescence...expressing YFP, CFP, and RFP are not present at the same frequencies. The small population size of CFP++ cells...fluorophores RFP (red), YFP (yellow), and membrane-tethered CFP (cyan). Brainbow 1.1 adds OFP (orange) (Livet et ...to localize to a different area of the cell than CFP and YFP. The most recent derivation of Brainbow,...construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous promoter. Zebrabow...that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific...construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP. After crossing onto a tamoxifen-inducible...
  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm / em 527 nm) can be imaged together but CFP and GFP...for multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed...
  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...with significant overlap in their spectra. The pair CFP-YFP was the first to be used to study protein-protein... mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs ...brightness - even though in the most popular pair, CFP has five-fold less brightness than YFP. The stoichiometry...
  4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered cyan). Without Cre recombination...can mediate one of two deletions to allow YFP or M-CFP expression; these deletions are defined using 2 loxP...express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem invertible segments...
  5. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived. For a breakdown...FRET occurs when energy transfer from one FP (e.g. CFP) excites the fluorescence of another FP (e.g. YFP...
  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...ProCFPp303 HTT CFP GAL1 Huntington's Susan Lindquist 15575 GAL 103Q+ProCFPp303 HTT CFP GAL1 Huntington's...HA25QDProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist 15579 GAL HA103QDProCFPp303 HTT HA, CFP GAL1 Huntington's... HTT FLAG, CFP GAL1 Huntington's Susan Lindquist 15585 GAL FLAG46QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's... HTT FLAG, CFP MET1 Huntington's Susan Lindquist 15590 MET FLAG72QDProCFPp303 HTT FLAG, CFP MET1 Huntington's...Parkinson's Richard Youle 23966 pTreTight-Htt94Q-CFP HTT CFP TREtight Huntington's Nico Dantuma 23971 VCP(... GFP CMV Parkinson's Richard Youle 47560 CFP-Parkin PRKN CFP CMV Parkinson's Richard Youle 50487 pSLIK-NFLAG-hFUS...Susan Lindquist 15570 GAL HA25Q+ProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist 15572 GAL FLAG25Q...
  7. Bacterial Expression Systems

    Type
    Collection
    ...18855 FLIParaF.Ec-250n Arabinose FRET fluorescence (CFP and Venus) Escherichia coli Wolf Frommer 20336 pRsetB-his7...
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