Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 637 results for: ESP

Showing: 1 - 20 of 637 results

  1. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...libro electrónico CRISPR 101 ya está disponible en español! Esta traducción fue regalada a Addgene por Daniel...Sánchez, ¡y no podríamos estar más emocionados! Después de recibir la traducción, Addgene contrató a una...

  2. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...of the Cas9 nuclease with engineered variants: eSpCas9, SpCas9-HF1, & HypaCas9. The off-target problem... sometimes even increase off-target effects, respectively. Recognizing these issues, researchers from ...the Cas9 nuclease itself. Design and testing of eSpCas9 Looking at the structure of the Cas9 nuclease (...testing of two of the mutants, SpCas9(K855A) and eSpCas9(1.1), followed by a technique for detecting genome-wide...precise mutations were different than those found in eSpCas9, the effect on specificity was similar; SpCas9-...important for nuclease specificity. They found that eSpCas9(1.1) and SpCas9-HF1 each stall in an inactive form...activity across the sites tested when compared to eSpCas9(1.1) and SpCas9-HF1. The HypaCas9 plasmids are ...

  3. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ... and the development of neutralizing antibody responses in patients. They were able to tweak this assay...effective or not. This protein both elicits an immune response as the target of vaccines and allows the virus....1.351 variant was very resistant to vaccine serum responses. This result was similar to studies that used ...context, what we showed was that the antibody responses are really not sufficient after one vaccine to...second vaccine dose in mounting a robust immune response. The Balazs lab was quick to deposit their plasmids...

  4. Antibody Guide

    Type
    Collection
    ...animal, which then mounts an immune response to it. Once the immune response has developed, the antibodies ...Antibodies, produced as part of the immune system’s response to foreign bodies, are the most commonly used ...Figure 1. The two arms of the Y structure are responsible for binding the antigen, and are called the antigen...bind to a specific antigen, triggering an immune response. The stem of the Y structure and the lower part...isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can change the constant...binding site. In B cells, the genetic region responsible for the variable region goes through a randomized...Different isotypes play different roles in the immune response, and can be used to increase flexibility or specificity...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...effects from repurposing endogenous systems that respond to natural stimuli like hormones or metal ions,...with a variety of improvements. A synthetic Tet Response Element (TRE) typically features seven copies ...has been modified to provide two classes of tet-responsive systems: Tet-Off and Tet-On. Read on to learn...mechanism; center: Tet-Off; right: Tet-On. TRE: Tet response element; tTA: tetracycline-controlled transactivator...Tet-On: express an rtTA or TetR and include a tet-responsive promoter upstream of your gene of interest. If... use Tet-Off: express a tTA and include a tet-responsive promoter upstream of your gene of interest. Many...whether they are used alongside a tTA or rtTA, respectively. You should also choose between: Dual vector...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...pathogen, the adaptive immune response kicks in. The adaptive immune response is antigen-specific and includes...rid of infectious pathogens. The innate immune response is triggered within the first few minutes of exposure...infection and rapidly initiate a secondary immune response. Browse Plasmids for Immunology Research Genes...Antigen Presentation The cornerstone of the immune response is the recognition by T cells of foreign antigens... cytokines that are released tailor the immune response to a specific trigger. Interferons The interferon...Type III interferons. They trigger the defensive responses of the immune system upon infection with virus...between immune cells over the course of an immune response. Over 60 interleukins have been described. NK ...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...slowing down. This prokaryotic immune system is especially amenable to genome engineering, offering flexibility...precisely target CRISPR to a given locus makes it especially amenable to genetic screens. Pooled gRNA libraries...Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target activity... in agricultural and therapeutic applications. Despite the ethical controversies surrounding non-research.... 1987. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in...

  8. Genetic Code Expansion

    Type
    Collection
    ...regulate the activity of a protein so that it is responsive to specific stimuli, such as light. On a broader...Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA synthetase...TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine...azidonorleucine (Anl) Mammalian Elizabeth Kirby 223508 pCMV_NESPylRS(AF)_hU6tRNAPyl PylRS (Y306A, Y384F) M. mazei...mazei Mammalian TAG Edward Lemke 223509 pCMV_NESPylRS(WT)_hU6tRNAPyl PylRS M. mazei Mammalian TAG Edward ...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ... Plasmid Collection Luciferase is the enzyme responsible for bioluminescence found in a variety of organisms...Click-beetle luciferases ( CBRluc and CBGluc , respectively), Cypridina luciferase , and Luciola luciferase... due to their large size (61 kDa and 36 kDa, respectively). And they are not secreted, so cells must be...Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling...

  10. Chemogenetics Plasmids

    Type
    Collection
    ...protein receptors that have been engineered to respond to specific physiologically inert small molecules... and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid receptor and...nicotinic acetylcholine receptor (nAChR) domains that respond specifically to small molecules termed Pharmacologically...

  11. Optogenetics Plasmid Collection

    Type
    Collection
    ...archaebacteria, that can be opened and closed in response to light. These proteins can be expressed in specific...reinhardtii . ChR2 is a membrane channel that opens in response to blue light and allows cations to flow into ...proteins. For a full description (including peak response spectra) of different types of opsins and optical...

  12. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Flow Cytometer For Sorting On Transient Cellular Responses Of Genetically-Encoded Sensors. Anal Chem. 2017...High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors for Citrate. ACS... ) Hydrogen peroxide biosensor HyPer7 Caveolae respond to acute oxidative stress through membrane lipid...single-fluorophore sensor for pyruvate A highly responsive pyruvate sensor reveals pathway-regulatory role...temperature indicator exploiting a temperature-responsive elastin-like polypeptide. Sci Rep. 2021 Aug 13...

  13. Ras Pathway

    Type
    Collection
    ...this page were generated with the help of Dominic Esposito and the Frederick National Laboratory for Cancer... Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway® Cloning Platform...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...C-terminal tal protein segments (136AA and 63AA, respectfully). This next generation architecture has been... contain TALE fused with mClover and mRuby2, respectively, and can be used as destination vectors for ...hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction sites for full compatibility...

  15. COVID-19 Resources

    Type
    Collection
    ... SARS-CoV-2 infection induces a cytokine storm response in the body. For a full list of cytokines and ...Cathepsin L Functionally Cleaves the Severe Acute Respiratory Syndrome Coronavirus Class I Fusion Protein Upstream...TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. . PCP4 - ...

  16. Plan Your Experiment

    Type
    Collection
    ...manipulations used in mammalian cells and their corresponding Cas enzymes. In the next section, we will cover...transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host ...necessary to optimize your delivery conditions, especially if you are using a difficult cell type. For more...

  17. Adenovirus Plasmids

    Type
    Collection
    ...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses... expression, as well as the substantial immune response induced by the viral capsid. Adenoviruses can ...

  18. Viral Vector Guides and Plasmids

    Type
    Collection
    ...expression.They can induce a strong immunogenic response, useful for clinical applications as vaccines.... Cells Infected Host Genome Integration Immune Response Lentiviral Stable RNA < 8 kb 80–130 nm Dividing...

  19. mTOR Pathway

    Type
    Collection
    ...mTORC1 & mTORC2 The better-characterized mTORC1 responds to various signals, including amino acids, stress...autophagy and lysosome biogenesis. mTORC2 does not respond to nutrients, but it is activated by growth factors...

  20. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...pegRNA-mCherry Mammalian hU6 pegRNA BpiI for pegRNA, Esp31 for PBS-RT No mCherry Ervin Welker 177181 pDAS12222...pegRNA-BFP Mammalian hU6 pegRNA BpiI for pegRNA, Esp31 for PBS-RT No BFP Ervin Welker Do you have suggestions...
Showing: 1 - 20 of 637 results